Lactococcus phage proPhi4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V2F9|A0A649V2F9_9CAUD Holin OS=Lactococcus phage proPhi4 OX=2656511 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.23RR3 pKa = 11.84PVEE6 pKa = 3.97IVQDD10 pKa = 4.02IIAASDD16 pKa = 3.89FPHH19 pKa = 7.8DD20 pKa = 4.81EE21 pKa = 4.16IFLDD25 pKa = 4.39SISSEE30 pKa = 4.14KK31 pKa = 10.5LDD33 pKa = 4.06SSNEE37 pKa = 4.12TQVLLTEE44 pKa = 4.58SDD46 pKa = 3.92NGPSDD51 pKa = 3.55YY52 pKa = 11.33GNSDD56 pKa = 5.04FISLMYY62 pKa = 10.15GVYY65 pKa = 9.99IQIFYY70 pKa = 11.34SNAEE74 pKa = 4.03DD75 pKa = 3.49SDD77 pKa = 4.11INIVQSEE84 pKa = 4.36INLMKK89 pKa = 10.79SFINNDD95 pKa = 2.8WLIAQSKK102 pKa = 7.64SHH104 pKa = 6.82YY105 pKa = 9.37IDD107 pKa = 5.43PDD109 pKa = 3.18TGQIIKK115 pKa = 10.36NLTVQRR121 pKa = 11.84IMTLSEE127 pKa = 3.84IANSS131 pKa = 3.58

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V2E5|A0A649V2E5_9CAUD Helicase subunit of the DNA excision repair complex OS=Lactococcus phage proPhi4 OX=2656511 PE=4 SV=1
MM1 pKa = 7.7GSLLLLMRR9 pKa = 11.84GDD11 pKa = 5.36AILPMTGRR19 pKa = 11.84CRR21 pKa = 11.84EE22 pKa = 4.3PNCHH26 pKa = 5.93AVVIRR31 pKa = 11.84PLHH34 pKa = 5.91YY35 pKa = 9.15CTKK38 pKa = 10.35HH39 pKa = 5.78ADD41 pKa = 3.25KK42 pKa = 10.53EE43 pKa = 4.12AAYY46 pKa = 9.83QASRR50 pKa = 11.84EE51 pKa = 3.88RR52 pKa = 11.84WTNRR56 pKa = 11.84TDD58 pKa = 2.89NSKK61 pKa = 10.61RR62 pKa = 11.84YY63 pKa = 9.85KK64 pKa = 10.51DD65 pKa = 3.41YY66 pKa = 11.27NKK68 pKa = 10.4RR69 pKa = 11.84KK70 pKa = 9.81RR71 pKa = 11.84EE72 pKa = 3.76YY73 pKa = 10.79SDD75 pKa = 3.68IKK77 pKa = 10.58VEE79 pKa = 4.11QNKK82 pKa = 9.42FYY84 pKa = 10.67QSKK87 pKa = 7.19QWKK90 pKa = 9.1SIRR93 pKa = 11.84DD94 pKa = 3.5VVRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84DD100 pKa = 3.45NFLCQYY106 pKa = 9.72CKK108 pKa = 10.03AHH110 pKa = 5.68NRR112 pKa = 11.84VRR114 pKa = 11.84TGKK117 pKa = 9.76IVDD120 pKa = 4.34HH121 pKa = 6.43IVPVEE126 pKa = 3.65FDD128 pKa = 4.25LNGKK132 pKa = 8.03TIMDD136 pKa = 4.16NLAFCCSKK144 pKa = 10.58CHH146 pKa = 5.55TRR148 pKa = 11.84KK149 pKa = 8.24TKK151 pKa = 9.8WEE153 pKa = 3.82QIYY156 pKa = 11.02YY157 pKa = 8.08GTGYY161 pKa = 10.91GNKK164 pKa = 8.24TKK166 pKa = 10.92NVIPIKK172 pKa = 10.2NVKK175 pKa = 9.75DD176 pKa = 3.58VPDD179 pKa = 3.84FQKK182 pKa = 11.1NEE184 pKa = 3.83RR185 pKa = 3.91

Molecular weight: 21.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11486

37

1640

234.4

26.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.834 ± 0.701

0.548 ± 0.142

6.382 ± 0.306

6.356 ± 0.52

3.935 ± 0.273

6.913 ± 0.533

1.332 ± 0.143

6.852 ± 0.347

8.645 ± 0.596

7.714 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.438 ± 0.176

6.495 ± 0.356

2.864 ± 0.269

4.371 ± 0.357

3.221 ± 0.3

7.557 ± 0.35

6.982 ± 0.603

5.703 ± 0.246

1.236 ± 0.143

3.604 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski