Lymphocystis disease virus 1 (isolate Darai) (LCDV-1)
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P22176|MCP_LCDV1 Major capsid protein OS=Lymphocystis disease virus 1 (isolate Darai) OX=654922 GN=MCP PE=3 SV=2
MM1 pKa = 7.24 TSVAGSSVTSAFIDD15 pKa = 3.72 LATYY19 pKa = 7.97 DD20 pKa = 3.97 TIEE23 pKa = 3.76 KK24 pKa = 9.71 HH25 pKa = 6.62 LYY27 pKa = 10.27 GGDD30 pKa = 3.36 SAVAYY35 pKa = 7.85 FVRR38 pKa = 11.84 EE39 pKa = 4.19 TKK41 pKa = 10.35 KK42 pKa = 9.82 CTWFSKK48 pKa = 10.81 LPVLLTRR55 pKa = 11.84 CSGSPNFDD63 pKa = 3.01 QEE65 pKa = 4.08 FSVNVSRR72 pKa = 11.84 GGDD75 pKa = 3.44 YY76 pKa = 11.21 VLNSWMTVRR85 pKa = 11.84 IPAIKK90 pKa = 10.27 LKK92 pKa = 10.71 ADD94 pKa = 2.89 NRR96 pKa = 11.84 MNNNGTIRR104 pKa = 11.84 WCKK107 pKa = 10.31 NLFHH111 pKa = 6.88 NLIKK115 pKa = 9.62 QTSVQFNDD123 pKa = 3.47 LVAQKK128 pKa = 10.5 FEE130 pKa = 5.3 SYY132 pKa = 10.83 FLDD135 pKa = 3.35 YY136 pKa = 10.0 WAAFSMCGSKK146 pKa = 10.4 RR147 pKa = 11.84 AGYY150 pKa = 11.14 NNMIGNTIDD159 pKa = 3.38 MIQPVDD165 pKa = 3.67 HH166 pKa = 6.82 TGMLPEE172 pKa = 4.59 KK173 pKa = 10.59 VLVLPLPYY181 pKa = 10.21 FFSRR185 pKa = 11.84 DD186 pKa = 3.25 SGVALPSAALPYY198 pKa = 10.7 NEE200 pKa = 4.64 IRR202 pKa = 11.84 LTFHH206 pKa = 6.57 LRR208 pKa = 11.84 DD209 pKa = 3.7 YY210 pKa = 11.08 TEE212 pKa = 4.81 LLIFQHH218 pKa = 6.5 KK219 pKa = 8.97 QDD221 pKa = 4.01 CTIIPITAADD231 pKa = 4.07 LEE233 pKa = 4.54 YY234 pKa = 11.02 GKK236 pKa = 9.76 PDD238 pKa = 3.59 LKK240 pKa = 10.95 DD241 pKa = 3.36 VQVWITNAVVTNEE254 pKa = 3.67 EE255 pKa = 4.03 RR256 pKa = 11.84 RR257 pKa = 11.84 LMGTTPRR264 pKa = 11.84 DD265 pKa = 3.28 ILVEE269 pKa = 4.03 QVQTAPKK276 pKa = 10.29 HH277 pKa = 5.01 VFQPLTIPSPNFDD290 pKa = 3.04 IRR292 pKa = 11.84 FSHH295 pKa = 7.48 AIKK298 pKa = 10.7 LLFFGVRR305 pKa = 11.84 NTTHH309 pKa = 7.21 AAVQSNYY316 pKa = 6.92 TTASPVILEE325 pKa = 3.97 EE326 pKa = 5.07 AYY328 pKa = 10.47 ASDD331 pKa = 4.27 LSLVAADD338 pKa = 5.15 PIANVTLVYY347 pKa = 10.59 EE348 pKa = 4.26 NSARR352 pKa = 11.84 LNEE355 pKa = 4.4 MGSEE359 pKa = 4.67 YY360 pKa = 10.79 YY361 pKa = 10.93 SLVQPYY367 pKa = 9.42 YY368 pKa = 9.95 FGGSIPIEE376 pKa = 4.35 TGYY379 pKa = 11.37 HH380 pKa = 5.3 MYY382 pKa = 10.37 CYY384 pKa = 9.73 SLNMMDD390 pKa = 4.77 MDD392 pKa = 4.67 PMGSTNYY399 pKa = 9.82 GRR401 pKa = 11.84 LSNVSMKK408 pKa = 10.94 LKK410 pKa = 9.92 TSDD413 pKa = 3.5 KK414 pKa = 10.95 AVVNAGGGGGNMSGYY429 pKa = 10.72 KK430 pKa = 9.85 DD431 pKa = 3.34 AQKK434 pKa = 11.15 FEE436 pKa = 4.39 FLTMAINHH444 pKa = 5.28 NVIRR448 pKa = 11.84 IKK450 pKa = 10.44 NGSMGFPVLL459 pKa = 3.96
Molecular weight: 51.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.091
IPC2_protein 6.135
IPC_protein 6.249
Toseland 6.547
ProMoST 6.649
Dawson 6.605
Bjellqvist 6.561
Wikipedia 6.62
Rodwell 6.59
Grimsley 6.678
Solomon 6.605
Lehninger 6.605
Nozaki 6.868
DTASelect 7.044
Thurlkill 7.059
EMBOSS 7.044
Sillero 6.985
Patrickios 3.783
IPC_peptide 6.62
IPC2_peptide 6.81
IPC2.peptide.svr19 6.809
Protein with the highest isoelectric point:
>sp|P22176|MCP_LCDV1 Major capsid protein OS=Lymphocystis disease virus 1 (isolate Darai) OX=654922 GN=MCP PE=3 SV=2
MM1 pKa = 7.24 TSVAGSSVTSAFIDD15 pKa = 3.72 LATYY19 pKa = 7.97 DD20 pKa = 3.97 TIEE23 pKa = 3.76 KK24 pKa = 9.71 HH25 pKa = 6.62 LYY27 pKa = 10.27 GGDD30 pKa = 3.36 SAVAYY35 pKa = 7.85 FVRR38 pKa = 11.84 EE39 pKa = 4.19 TKK41 pKa = 10.35 KK42 pKa = 9.82 CTWFSKK48 pKa = 10.81 LPVLLTRR55 pKa = 11.84 CSGSPNFDD63 pKa = 3.01 QEE65 pKa = 4.08 FSVNVSRR72 pKa = 11.84 GGDD75 pKa = 3.44 YY76 pKa = 11.21 VLNSWMTVRR85 pKa = 11.84 IPAIKK90 pKa = 10.27 LKK92 pKa = 10.71 ADD94 pKa = 2.89 NRR96 pKa = 11.84 MNNNGTIRR104 pKa = 11.84 WCKK107 pKa = 10.31 NLFHH111 pKa = 6.88 NLIKK115 pKa = 9.62 QTSVQFNDD123 pKa = 3.47 LVAQKK128 pKa = 10.5 FEE130 pKa = 5.3 SYY132 pKa = 10.83 FLDD135 pKa = 3.35 YY136 pKa = 10.0 WAAFSMCGSKK146 pKa = 10.4 RR147 pKa = 11.84 AGYY150 pKa = 11.14 NNMIGNTIDD159 pKa = 3.38 MIQPVDD165 pKa = 3.67 HH166 pKa = 6.82 TGMLPEE172 pKa = 4.59 KK173 pKa = 10.59 VLVLPLPYY181 pKa = 10.21 FFSRR185 pKa = 11.84 DD186 pKa = 3.25 SGVALPSAALPYY198 pKa = 10.7 NEE200 pKa = 4.64 IRR202 pKa = 11.84 LTFHH206 pKa = 6.57 LRR208 pKa = 11.84 DD209 pKa = 3.7 YY210 pKa = 11.08 TEE212 pKa = 4.81 LLIFQHH218 pKa = 6.5 KK219 pKa = 8.97 QDD221 pKa = 4.01 CTIIPITAADD231 pKa = 4.07 LEE233 pKa = 4.54 YY234 pKa = 11.02 GKK236 pKa = 9.76 PDD238 pKa = 3.59 LKK240 pKa = 10.95 DD241 pKa = 3.36 VQVWITNAVVTNEE254 pKa = 3.67 EE255 pKa = 4.03 RR256 pKa = 11.84 RR257 pKa = 11.84 LMGTTPRR264 pKa = 11.84 DD265 pKa = 3.28 ILVEE269 pKa = 4.03 QVQTAPKK276 pKa = 10.29 HH277 pKa = 5.01 VFQPLTIPSPNFDD290 pKa = 3.04 IRR292 pKa = 11.84 FSHH295 pKa = 7.48 AIKK298 pKa = 10.7 LLFFGVRR305 pKa = 11.84 NTTHH309 pKa = 7.21 AAVQSNYY316 pKa = 6.92 TTASPVILEE325 pKa = 3.97 EE326 pKa = 5.07 AYY328 pKa = 10.47 ASDD331 pKa = 4.27 LSLVAADD338 pKa = 5.15 PIANVTLVYY347 pKa = 10.59 EE348 pKa = 4.26 NSARR352 pKa = 11.84 LNEE355 pKa = 4.4 MGSEE359 pKa = 4.67 YY360 pKa = 10.79 YY361 pKa = 10.93 SLVQPYY367 pKa = 9.42 YY368 pKa = 9.95 FGGSIPIEE376 pKa = 4.35 TGYY379 pKa = 11.37 HH380 pKa = 5.3 MYY382 pKa = 10.37 CYY384 pKa = 9.73 SLNMMDD390 pKa = 4.77 MDD392 pKa = 4.67 PMGSTNYY399 pKa = 9.82 GRR401 pKa = 11.84 LSNVSMKK408 pKa = 10.94 LKK410 pKa = 9.92 TSDD413 pKa = 3.5 KK414 pKa = 10.95 AVVNAGGGGGNMSGYY429 pKa = 10.72 KK430 pKa = 9.85 DD431 pKa = 3.34 AQKK434 pKa = 11.15 FEE436 pKa = 4.39 FLTMAINHH444 pKa = 5.28 NVIRR448 pKa = 11.84 IKK450 pKa = 10.44 NGSMGFPVLL459 pKa = 3.96
Molecular weight: 51.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.091
IPC2_protein 6.135
IPC_protein 6.249
Toseland 6.547
ProMoST 6.649
Dawson 6.605
Bjellqvist 6.561
Wikipedia 6.62
Rodwell 6.59
Grimsley 6.678
Solomon 6.605
Lehninger 6.605
Nozaki 6.868
DTASelect 7.044
Thurlkill 7.059
EMBOSS 7.044
Sillero 6.985
Patrickios 3.783
IPC_peptide 6.62
IPC2_peptide 6.81
IPC2.peptide.svr19 6.809
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
459
459
459
459.0
51.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.972 ± 0.0
1.307 ± 0.0
5.229 ± 0.0
3.922 ± 0.0
5.011 ± 0.0
6.318 ± 0.0
2.179 ± 0.0
5.664 ± 0.0
4.793 ± 0.0
8.497 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.922 ± 0.0
6.536 ± 0.0
4.793 ± 0.0
3.05 ± 0.0
3.922 ± 0.0
7.625 ± 0.0
6.754 ± 0.0
7.407 ± 0.0
1.089 ± 0.0
5.011 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here