Wolbachia endosymbiont of Onchocerca ochengi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Wolbachieae; Wolbachia; unclassified Wolbachia

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 647 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7JF30|I7JF30_9RICK Cytochrome aa3 subunit 3 OS=Wolbachia endosymbiont of Onchocerca ochengi OX=100901 GN=wOo_08890 PE=3 SV=1
MM1 pKa = 7.52SSEE4 pKa = 4.08VQGPNIAEE12 pKa = 3.93WQAQQQHH19 pKa = 5.25QEE21 pKa = 4.12YY22 pKa = 10.03QGGKK26 pKa = 8.98SGFGLGHH33 pKa = 6.56SDD35 pKa = 3.35SVATIVDD42 pKa = 3.45GMKK45 pKa = 9.62WLFSFHH51 pKa = 6.65EE52 pKa = 4.56GAITSYY58 pKa = 11.36NNVFEE63 pKa = 4.35GLISGSSLVTLFGKK77 pKa = 9.67SGNMFDD83 pKa = 3.99IKK85 pKa = 10.59FLEE88 pKa = 4.44GLAEE92 pKa = 4.19HH93 pKa = 6.85FSGGGGDD100 pKa = 3.97GEE102 pKa = 5.18GITLEE107 pKa = 4.21EE108 pKa = 3.89TDD110 pKa = 3.43MGYY113 pKa = 10.91SNKK116 pKa = 9.72GSHH119 pKa = 7.01LDD121 pKa = 3.73DD122 pKa = 4.26YY123 pKa = 11.37SNSSSEE129 pKa = 4.16IAHH132 pKa = 6.28SADD135 pKa = 3.31GLHH138 pKa = 6.98GIHH141 pKa = 6.87NSGDD145 pKa = 3.45YY146 pKa = 10.07HH147 pKa = 7.04DD148 pKa = 5.79CSFSPSPSPSVGDD161 pKa = 3.38DD162 pKa = 2.95HH163 pKa = 9.09GYY165 pKa = 10.04EE166 pKa = 4.06LSVV169 pKa = 3.28

Molecular weight: 17.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7IAI5|I7IAI5_9RICK Lipoprotein OsmY ortholog OS=Wolbachia endosymbiont of Onchocerca ochengi OX=100901 GN=wOo_09480 PE=4 SV=1
MM1 pKa = 6.87VKK3 pKa = 10.08KK4 pKa = 10.45RR5 pKa = 11.84NSFNDD10 pKa = 3.1SYY12 pKa = 11.93LSMNNRR18 pKa = 11.84TGFRR22 pKa = 11.84RR23 pKa = 11.84SKK25 pKa = 10.4ACPLATSKK33 pKa = 11.5DD34 pKa = 3.19EE35 pKa = 5.55DD36 pKa = 3.36IDD38 pKa = 4.03YY39 pKa = 11.23KK40 pKa = 10.87NTDD43 pKa = 4.11LLSKK47 pKa = 9.3FTSDD51 pKa = 3.32YY52 pKa = 11.28GRR54 pKa = 11.84ILPRR58 pKa = 11.84RR59 pKa = 11.84LTGVCARR66 pKa = 11.84RR67 pKa = 11.84QRR69 pKa = 11.84KK70 pKa = 8.36LRR72 pKa = 11.84LAIIRR77 pKa = 11.84ARR79 pKa = 11.84FLALVPYY86 pKa = 7.91CTKK89 pKa = 10.62KK90 pKa = 10.81AA91 pKa = 3.45

Molecular weight: 10.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

647

0

647

211834

45

2840

327.4

36.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.757 ± 0.075

1.386 ± 0.036

4.838 ± 0.063

6.172 ± 0.097

4.653 ± 0.072

5.921 ± 0.072

1.879 ± 0.032

9.667 ± 0.086

8.413 ± 0.085

9.757 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.035

5.927 ± 0.067

3.053 ± 0.043

2.907 ± 0.042

4.07 ± 0.061

7.622 ± 0.067

4.661 ± 0.049

6.689 ± 0.066

0.779 ± 0.029

3.605 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski