HIV-1 M_02CD.LBTB032

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 group M unknown subgroup

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7TQ80|C7TQ80_9HIV1 Protein Nef OS=HIV-1 M_02CD.LBTB032 OX=576899 GN=nef PE=2 SV=1
MM1 pKa = 7.2GGKK4 pKa = 8.6WSKK7 pKa = 10.93SSIVGWPDD15 pKa = 2.52VRR17 pKa = 11.84EE18 pKa = 4.02RR19 pKa = 11.84MRR21 pKa = 11.84RR22 pKa = 11.84ASPAAEE28 pKa = 3.89GVGAASKK35 pKa = 11.07DD36 pKa = 3.38LDD38 pKa = 3.66KK39 pKa = 11.39FGAITSSNTPSTNADD54 pKa = 3.97CAWLEE59 pKa = 3.94AQEE62 pKa = 4.38EE63 pKa = 4.4EE64 pKa = 4.77GGVGFPVRR72 pKa = 11.84PQVPLRR78 pKa = 11.84PMTYY82 pKa = 9.94KK83 pKa = 10.84GAFDD87 pKa = 4.45LGFFLKK93 pKa = 10.62EE94 pKa = 3.8KK95 pKa = 10.62GGLDD99 pKa = 3.19GLIYY103 pKa = 10.29SKK105 pKa = 10.58KK106 pKa = 9.34RR107 pKa = 11.84QEE109 pKa = 5.1ILDD112 pKa = 3.35LWVYY116 pKa = 8.03HH117 pKa = 5.36TQGFFPDD124 pKa = 3.52WQNYY128 pKa = 6.23TPGPGIRR135 pKa = 11.84YY136 pKa = 7.88PLTFGWPYY144 pKa = 11.05KK145 pKa = 10.12LVPVDD150 pKa = 3.38PRR152 pKa = 11.84EE153 pKa = 3.95VEE155 pKa = 3.99KK156 pKa = 11.32ANEE159 pKa = 4.24GEE161 pKa = 4.39NNCLLHH167 pKa = 6.82PMSQHH172 pKa = 5.14GMEE175 pKa = 4.31DD176 pKa = 3.4EE177 pKa = 4.04EE178 pKa = 5.55RR179 pKa = 11.84EE180 pKa = 4.14VLKK183 pKa = 10.57WVFDD187 pKa = 3.57SSLARR192 pKa = 11.84EE193 pKa = 4.37HH194 pKa = 6.41VAHH197 pKa = 7.22KK198 pKa = 9.39LHH200 pKa = 6.69PEE202 pKa = 4.05YY203 pKa = 11.29YY204 pKa = 9.9KK205 pKa = 11.0DD206 pKa = 3.59CC207 pKa = 4.69

Molecular weight: 23.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7TQ77|C7TQ77_9HIV1 Protein Vpr OS=HIV-1 M_02CD.LBTB032 OX=576899 GN=vpr PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.04RR15 pKa = 11.84MRR17 pKa = 11.84IRR19 pKa = 11.84TWHH22 pKa = 6.66SIVKK26 pKa = 8.9HH27 pKa = 4.93HH28 pKa = 6.81MYY30 pKa = 10.24RR31 pKa = 11.84SKK33 pKa = 10.7KK34 pKa = 9.15AKK36 pKa = 8.17GWFYY40 pKa = 9.7RR41 pKa = 11.84HH42 pKa = 6.47HH43 pKa = 6.67YY44 pKa = 9.24EE45 pKa = 3.91SRR47 pKa = 11.84HH48 pKa = 5.47PKK50 pKa = 9.03VCSEE54 pKa = 3.54VHH56 pKa = 6.02IPIEE60 pKa = 3.93EE61 pKa = 3.87ARR63 pKa = 11.84LVIRR67 pKa = 11.84TYY69 pKa = 10.14WGLQPGEE76 pKa = 4.38KK77 pKa = 9.21AWHH80 pKa = 6.52LGHH83 pKa = 6.45GVSIEE88 pKa = 3.89WSQGKK93 pKa = 10.03YY94 pKa = 7.45STQVDD99 pKa = 3.99PDD101 pKa = 3.78LADD104 pKa = 3.51QLIHH108 pKa = 6.53LQYY111 pKa = 10.4FDD113 pKa = 4.94CFSEE117 pKa = 4.22STIRR121 pKa = 11.84KK122 pKa = 9.18AILGHH127 pKa = 6.64RR128 pKa = 11.84IIHH131 pKa = 5.5RR132 pKa = 11.84CEE134 pKa = 3.74YY135 pKa = 9.94QAGHH139 pKa = 6.08SKK141 pKa = 10.63VGSLQYY147 pKa = 10.76LALKK151 pKa = 9.2TLVEE155 pKa = 4.17PRR157 pKa = 11.84GTKK160 pKa = 9.95PPLPSVRR167 pKa = 11.84KK168 pKa = 8.46LTEE171 pKa = 4.14DD172 pKa = 3.16RR173 pKa = 11.84WNEE176 pKa = 3.69PQKK179 pKa = 9.76TRR181 pKa = 11.84GRR183 pKa = 11.84RR184 pKa = 11.84GNHH187 pKa = 5.44SMNGHH192 pKa = 6.49

Molecular weight: 22.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2868

81

1431

478.0

54.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.137 ± 0.449

1.743 ± 0.358

4.393 ± 0.355

6.625 ± 0.589

2.755 ± 0.327

7.497 ± 0.349

2.406 ± 0.986

7.566 ± 0.674

6.974 ± 1.088

8.089 ± 0.631

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.987 ± 0.098

5.021 ± 1.114

5.021 ± 0.573

5.579 ± 0.69

5.474 ± 0.934

4.916 ± 0.368

5.788 ± 0.592

6.311 ± 0.322

2.929 ± 0.246

2.72 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski