Cronartium ribicola mitovirus 1
Average proteome isoelectric point is 8.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KCP6|A0A191KCP6_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 1 OX=1816484 GN=RdRp PE=4 SV=1
MM1 pKa = 7.61 RR2 pKa = 11.84 MKK4 pKa = 10.65 SRR6 pKa = 11.84 TWIQASSLLVKK17 pKa = 10.67 ISLPKK22 pKa = 10.3 DD23 pKa = 3.41 LQSCVHH29 pKa = 6.54 ILALLSVYY37 pKa = 9.91 WEE39 pKa = 4.35 IDD41 pKa = 3.3 SSDD44 pKa = 3.96 WIPYY48 pKa = 8.2 IDD50 pKa = 3.32 RR51 pKa = 11.84 VVKK54 pKa = 10.45 IRR56 pKa = 11.84 RR57 pKa = 11.84 LSGTPYY63 pKa = 7.86 TVKK66 pKa = 9.79 WNKK69 pKa = 9.53 SNRR72 pKa = 11.84 LAFTRR77 pKa = 11.84 WMCGHH82 pKa = 7.41 PLKK85 pKa = 10.93 EE86 pKa = 4.28 SDD88 pKa = 4.28 VPLHH92 pKa = 6.88 KK93 pKa = 10.86 DD94 pKa = 3.37 GFPRR98 pKa = 11.84 NLSSMKK104 pKa = 10.17 KK105 pKa = 7.39 YY106 pKa = 8.89 TSSARR111 pKa = 11.84 GIAFLLTLLIWSRR124 pKa = 11.84 GLHH127 pKa = 5.05 QWSKK131 pKa = 10.62 PDD133 pKa = 3.12 ISAVLSPYY141 pKa = 8.12 TGKK144 pKa = 8.06 MTEE147 pKa = 4.64 DD148 pKa = 2.88 KK149 pKa = 10.19 WSRR152 pKa = 11.84 SPVQFLADD160 pKa = 3.68 RR161 pKa = 11.84 VRR163 pKa = 11.84 LSLGKK168 pKa = 9.28 PLEE171 pKa = 4.42 PWSRR175 pKa = 11.84 WHH177 pKa = 5.67 LTTRR181 pKa = 11.84 NGPNGHH187 pKa = 7.15 ALTGILEE194 pKa = 4.3 DD195 pKa = 4.29 LKK197 pKa = 11.11 AITGSCIPHH206 pKa = 6.78 LTTLGGKK213 pKa = 10.11 KK214 pKa = 9.25 IVTYY218 pKa = 10.64 LSHH221 pKa = 7.29 FKK223 pKa = 11.0 VLTSKK228 pKa = 10.7 EE229 pKa = 3.72 GALNYY234 pKa = 9.91 IKK236 pKa = 10.52 EE237 pKa = 4.3 RR238 pKa = 11.84 LSLRR242 pKa = 11.84 YY243 pKa = 9.5 QKK245 pKa = 10.54 EE246 pKa = 4.36 SPSRR250 pKa = 11.84 LAKK253 pKa = 10.68 LIFIKK258 pKa = 10.73 DD259 pKa = 3.47 KK260 pKa = 9.06 EE261 pKa = 4.3 LKK263 pKa = 9.16 MRR265 pKa = 11.84 SIGIGNYY272 pKa = 8.37 WYY274 pKa = 10.44 QAALKK279 pKa = 9.63 NLHH282 pKa = 6.78 DD283 pKa = 3.96 QLIDD287 pKa = 3.05 ILRR290 pKa = 11.84 RR291 pKa = 11.84 IPEE294 pKa = 4.64 DD295 pKa = 3.21 ATFDD299 pKa = 3.32 QGKK302 pKa = 9.74 APRR305 pKa = 11.84 VLRR308 pKa = 11.84 KK309 pKa = 9.79 PEE311 pKa = 3.82 GHH313 pKa = 6.48 SYY315 pKa = 10.48 WSMDD319 pKa = 3.0 LTAATDD325 pKa = 4.88 RR326 pKa = 11.84 FPLWYY331 pKa = 8.23 QKK333 pKa = 11.05 KK334 pKa = 10.41 VIGEE338 pKa = 4.15 LFSAEE343 pKa = 4.17 VAEE346 pKa = 4.23 SWSYY350 pKa = 11.23 IITTKK355 pKa = 10.45 FSSRR359 pKa = 11.84 EE360 pKa = 3.81 FGEE363 pKa = 3.85 VKK365 pKa = 10.58 YY366 pKa = 10.88 AVGQPIGFYY375 pKa = 10.66 SSWAAFAVSHH385 pKa = 6.63 HH386 pKa = 6.22 IVVHH390 pKa = 5.79 AAAIKK395 pKa = 10.62 AGIRR399 pKa = 11.84 DD400 pKa = 3.84 LKK402 pKa = 10.69 GLYY405 pKa = 9.82 VLLGDD410 pKa = 6.08 DD411 pKa = 4.89 IVLCHH416 pKa = 7.4 DD417 pKa = 4.18 DD418 pKa = 3.5 LARR421 pKa = 11.84 EE422 pKa = 3.97 YY423 pKa = 10.84 KK424 pKa = 10.34 IIIGLLGVEE433 pKa = 4.23 LSISKK438 pKa = 7.58 THH440 pKa = 6.37 QSDD443 pKa = 3.12 HH444 pKa = 6.23 TYY446 pKa = 10.85 EE447 pKa = 4.14 FAKK450 pKa = 10.1 RR451 pKa = 11.84 WYY453 pKa = 8.35 SDD455 pKa = 2.38 KK456 pKa = 10.55 WGEE459 pKa = 3.78 ISPFPIHH466 pKa = 6.74 SVSPKK471 pKa = 10.62 AKK473 pKa = 8.2 YY474 pKa = 8.85 TDD476 pKa = 3.61 IIAIINSAAEE486 pKa = 4.44 KK487 pKa = 9.81 GWSPSSQHH495 pKa = 5.45 YY496 pKa = 11.06 LEE498 pKa = 5.15 FAAASNCLLHH508 pKa = 6.96 KK509 pKa = 10.4 NHH511 pKa = 6.74 GDD513 pKa = 3.31 EE514 pKa = 4.77 FYY516 pKa = 11.14 SRR518 pKa = 11.84 SLRR521 pKa = 11.84 FLNLYY526 pKa = 8.86 RR527 pKa = 11.84 LCFIASTGQGSWSEE541 pKa = 4.28 VIRR544 pKa = 11.84 NLDD547 pKa = 3.23 VARR550 pKa = 11.84 RR551 pKa = 11.84 TISVAGSAWYY561 pKa = 9.11 HH562 pKa = 5.55 PKK564 pKa = 10.66 YY565 pKa = 11.02 GEE567 pKa = 4.77 DD568 pKa = 3.85 LYY570 pKa = 11.51 QLTLLVIFLNKK581 pKa = 10.2 GVGKK585 pKa = 10.01 LATATKK591 pKa = 9.4 VASRR595 pKa = 11.84 TILNLIPRR603 pKa = 11.84 TAEE606 pKa = 3.73 PNHH609 pKa = 6.22 PCKK612 pKa = 10.68 GLWSVLINLPQSHH625 pKa = 6.44 IVSDD629 pKa = 3.76 IVDD632 pKa = 3.97 SFSKK636 pKa = 10.85 DD637 pKa = 3.08 SFLSLIQQYY646 pKa = 10.27 IGQDD650 pKa = 3.32 NKK652 pKa = 10.91 KK653 pKa = 9.71 LSSLITCDD661 pKa = 3.59 LNTVLTTRR669 pKa = 11.84 NQQLSWNVLHH679 pKa = 6.96 KK680 pKa = 10.37 VVQAIVVTISRR691 pKa = 11.84 VSEE694 pKa = 3.87 QGCTFRR700 pKa = 11.84 GPEE703 pKa = 3.93 QLPLEE708 pKa = 4.56 VVRR711 pKa = 11.84 AAFKK715 pKa = 10.25 PISNRR720 pKa = 11.84 SLAVGTRR727 pKa = 11.84 YY728 pKa = 9.96 VSSDD732 pKa = 3.31 NPLYY736 pKa = 10.79 SYY738 pKa = 9.42 WVEE741 pKa = 3.99 MKK743 pKa = 10.45 KK744 pKa = 10.65 LGVIGQTQMWVTAKK758 pKa = 10.01 TKK760 pKa = 10.37 GLQPLQQDD768 pKa = 4.75 TILDD772 pKa = 4.24 DD773 pKa = 4.34 PDD775 pKa = 4.2 FSIFSRR781 pKa = 11.84 IDD783 pKa = 3.33 YY784 pKa = 10.42 LDD786 pKa = 3.21 KK787 pKa = 10.41 TGKK790 pKa = 9.75 VSSYY794 pKa = 11.08 LYY796 pKa = 10.72 GEE798 pKa = 4.5 RR799 pKa = 11.84 GG800 pKa = 3.12
Molecular weight: 90.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.853
IPC2_protein 8.946
IPC_protein 8.829
Toseland 9.531
ProMoST 9.311
Dawson 9.809
Bjellqvist 9.531
Wikipedia 9.984
Rodwell 10.101
Grimsley 9.897
Solomon 9.823
Lehninger 9.78
Nozaki 9.604
DTASelect 9.502
Thurlkill 9.648
EMBOSS 9.955
Sillero 9.736
Patrickios 4.94
IPC_peptide 9.823
IPC2_peptide 8.126
IPC2.peptide.svr19 7.929
Protein with the highest isoelectric point:
>tr|A0A191KCP6|A0A191KCP6_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 1 OX=1816484 GN=RdRp PE=4 SV=1
MM1 pKa = 7.61 RR2 pKa = 11.84 MKK4 pKa = 10.65 SRR6 pKa = 11.84 TWIQASSLLVKK17 pKa = 10.67 ISLPKK22 pKa = 10.3 DD23 pKa = 3.41 LQSCVHH29 pKa = 6.54 ILALLSVYY37 pKa = 9.91 WEE39 pKa = 4.35 IDD41 pKa = 3.3 SSDD44 pKa = 3.96 WIPYY48 pKa = 8.2 IDD50 pKa = 3.32 RR51 pKa = 11.84 VVKK54 pKa = 10.45 IRR56 pKa = 11.84 RR57 pKa = 11.84 LSGTPYY63 pKa = 7.86 TVKK66 pKa = 9.79 WNKK69 pKa = 9.53 SNRR72 pKa = 11.84 LAFTRR77 pKa = 11.84 WMCGHH82 pKa = 7.41 PLKK85 pKa = 10.93 EE86 pKa = 4.28 SDD88 pKa = 4.28 VPLHH92 pKa = 6.88 KK93 pKa = 10.86 DD94 pKa = 3.37 GFPRR98 pKa = 11.84 NLSSMKK104 pKa = 10.17 KK105 pKa = 7.39 YY106 pKa = 8.89 TSSARR111 pKa = 11.84 GIAFLLTLLIWSRR124 pKa = 11.84 GLHH127 pKa = 5.05 QWSKK131 pKa = 10.62 PDD133 pKa = 3.12 ISAVLSPYY141 pKa = 8.12 TGKK144 pKa = 8.06 MTEE147 pKa = 4.64 DD148 pKa = 2.88 KK149 pKa = 10.19 WSRR152 pKa = 11.84 SPVQFLADD160 pKa = 3.68 RR161 pKa = 11.84 VRR163 pKa = 11.84 LSLGKK168 pKa = 9.28 PLEE171 pKa = 4.42 PWSRR175 pKa = 11.84 WHH177 pKa = 5.67 LTTRR181 pKa = 11.84 NGPNGHH187 pKa = 7.15 ALTGILEE194 pKa = 4.3 DD195 pKa = 4.29 LKK197 pKa = 11.11 AITGSCIPHH206 pKa = 6.78 LTTLGGKK213 pKa = 10.11 KK214 pKa = 9.25 IVTYY218 pKa = 10.64 LSHH221 pKa = 7.29 FKK223 pKa = 11.0 VLTSKK228 pKa = 10.7 EE229 pKa = 3.72 GALNYY234 pKa = 9.91 IKK236 pKa = 10.52 EE237 pKa = 4.3 RR238 pKa = 11.84 LSLRR242 pKa = 11.84 YY243 pKa = 9.5 QKK245 pKa = 10.54 EE246 pKa = 4.36 SPSRR250 pKa = 11.84 LAKK253 pKa = 10.68 LIFIKK258 pKa = 10.73 DD259 pKa = 3.47 KK260 pKa = 9.06 EE261 pKa = 4.3 LKK263 pKa = 9.16 MRR265 pKa = 11.84 SIGIGNYY272 pKa = 8.37 WYY274 pKa = 10.44 QAALKK279 pKa = 9.63 NLHH282 pKa = 6.78 DD283 pKa = 3.96 QLIDD287 pKa = 3.05 ILRR290 pKa = 11.84 RR291 pKa = 11.84 IPEE294 pKa = 4.64 DD295 pKa = 3.21 ATFDD299 pKa = 3.32 QGKK302 pKa = 9.74 APRR305 pKa = 11.84 VLRR308 pKa = 11.84 KK309 pKa = 9.79 PEE311 pKa = 3.82 GHH313 pKa = 6.48 SYY315 pKa = 10.48 WSMDD319 pKa = 3.0 LTAATDD325 pKa = 4.88 RR326 pKa = 11.84 FPLWYY331 pKa = 8.23 QKK333 pKa = 11.05 KK334 pKa = 10.41 VIGEE338 pKa = 4.15 LFSAEE343 pKa = 4.17 VAEE346 pKa = 4.23 SWSYY350 pKa = 11.23 IITTKK355 pKa = 10.45 FSSRR359 pKa = 11.84 EE360 pKa = 3.81 FGEE363 pKa = 3.85 VKK365 pKa = 10.58 YY366 pKa = 10.88 AVGQPIGFYY375 pKa = 10.66 SSWAAFAVSHH385 pKa = 6.63 HH386 pKa = 6.22 IVVHH390 pKa = 5.79 AAAIKK395 pKa = 10.62 AGIRR399 pKa = 11.84 DD400 pKa = 3.84 LKK402 pKa = 10.69 GLYY405 pKa = 9.82 VLLGDD410 pKa = 6.08 DD411 pKa = 4.89 IVLCHH416 pKa = 7.4 DD417 pKa = 4.18 DD418 pKa = 3.5 LARR421 pKa = 11.84 EE422 pKa = 3.97 YY423 pKa = 10.84 KK424 pKa = 10.34 IIIGLLGVEE433 pKa = 4.23 LSISKK438 pKa = 7.58 THH440 pKa = 6.37 QSDD443 pKa = 3.12 HH444 pKa = 6.23 TYY446 pKa = 10.85 EE447 pKa = 4.14 FAKK450 pKa = 10.1 RR451 pKa = 11.84 WYY453 pKa = 8.35 SDD455 pKa = 2.38 KK456 pKa = 10.55 WGEE459 pKa = 3.78 ISPFPIHH466 pKa = 6.74 SVSPKK471 pKa = 10.62 AKK473 pKa = 8.2 YY474 pKa = 8.85 TDD476 pKa = 3.61 IIAIINSAAEE486 pKa = 4.44 KK487 pKa = 9.81 GWSPSSQHH495 pKa = 5.45 YY496 pKa = 11.06 LEE498 pKa = 5.15 FAAASNCLLHH508 pKa = 6.96 KK509 pKa = 10.4 NHH511 pKa = 6.74 GDD513 pKa = 3.31 EE514 pKa = 4.77 FYY516 pKa = 11.14 SRR518 pKa = 11.84 SLRR521 pKa = 11.84 FLNLYY526 pKa = 8.86 RR527 pKa = 11.84 LCFIASTGQGSWSEE541 pKa = 4.28 VIRR544 pKa = 11.84 NLDD547 pKa = 3.23 VARR550 pKa = 11.84 RR551 pKa = 11.84 TISVAGSAWYY561 pKa = 9.11 HH562 pKa = 5.55 PKK564 pKa = 10.66 YY565 pKa = 11.02 GEE567 pKa = 4.77 DD568 pKa = 3.85 LYY570 pKa = 11.51 QLTLLVIFLNKK581 pKa = 10.2 GVGKK585 pKa = 10.01 LATATKK591 pKa = 9.4 VASRR595 pKa = 11.84 TILNLIPRR603 pKa = 11.84 TAEE606 pKa = 3.73 PNHH609 pKa = 6.22 PCKK612 pKa = 10.68 GLWSVLINLPQSHH625 pKa = 6.44 IVSDD629 pKa = 3.76 IVDD632 pKa = 3.97 SFSKK636 pKa = 10.85 DD637 pKa = 3.08 SFLSLIQQYY646 pKa = 10.27 IGQDD650 pKa = 3.32 NKK652 pKa = 10.91 KK653 pKa = 9.71 LSSLITCDD661 pKa = 3.59 LNTVLTTRR669 pKa = 11.84 NQQLSWNVLHH679 pKa = 6.96 KK680 pKa = 10.37 VVQAIVVTISRR691 pKa = 11.84 VSEE694 pKa = 3.87 QGCTFRR700 pKa = 11.84 GPEE703 pKa = 3.93 QLPLEE708 pKa = 4.56 VVRR711 pKa = 11.84 AAFKK715 pKa = 10.25 PISNRR720 pKa = 11.84 SLAVGTRR727 pKa = 11.84 YY728 pKa = 9.96 VSSDD732 pKa = 3.31 NPLYY736 pKa = 10.79 SYY738 pKa = 9.42 WVEE741 pKa = 3.99 MKK743 pKa = 10.45 KK744 pKa = 10.65 LGVIGQTQMWVTAKK758 pKa = 10.01 TKK760 pKa = 10.37 GLQPLQQDD768 pKa = 4.75 TILDD772 pKa = 4.24 DD773 pKa = 4.34 PDD775 pKa = 4.2 FSIFSRR781 pKa = 11.84 IDD783 pKa = 3.33 YY784 pKa = 10.42 LDD786 pKa = 3.21 KK787 pKa = 10.41 TGKK790 pKa = 9.75 VSSYY794 pKa = 11.08 LYY796 pKa = 10.72 GEE798 pKa = 4.5 RR799 pKa = 11.84 GG800 pKa = 3.12
Molecular weight: 90.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.853
IPC2_protein 8.946
IPC_protein 8.829
Toseland 9.531
ProMoST 9.311
Dawson 9.809
Bjellqvist 9.531
Wikipedia 9.984
Rodwell 10.101
Grimsley 9.897
Solomon 9.823
Lehninger 9.78
Nozaki 9.604
DTASelect 9.502
Thurlkill 9.648
EMBOSS 9.955
Sillero 9.736
Patrickios 4.94
IPC_peptide 9.823
IPC2_peptide 8.126
IPC2.peptide.svr19 7.929
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
800
800
800
800.0
90.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.125 ± 0.0
1.125 ± 0.0
5.0 ± 0.0
3.875 ± 0.0
3.25 ± 0.0
5.875 ± 0.0
3.0 ± 0.0
7.375 ± 0.0
7.25 ± 0.0
11.25 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.125 ± 0.0
2.875 ± 0.0
4.25 ± 0.0
3.5 ± 0.0
5.5 ± 0.0
10.125 ± 0.0
5.375 ± 0.0
6.0 ± 0.0
3.0 ± 0.0
4.125 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here