Lake Sarah-associated circular virus-24

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9Q8|A0A126G9Q8_9VIRU Coat protein OS=Lake Sarah-associated circular virus-24 OX=1685751 PE=4 SV=1
MM1 pKa = 7.75TDD3 pKa = 4.73RR4 pKa = 11.84ATCWSITINNPTEE17 pKa = 4.07EE18 pKa = 4.58EE19 pKa = 4.16VNAWTKK25 pKa = 10.21IPGWKK30 pKa = 8.44LTGQYY35 pKa = 10.77EE36 pKa = 4.35EE37 pKa = 4.74GDD39 pKa = 3.74KK40 pKa = 11.06TGTPHH45 pKa = 5.9FQGMLQTPQVRR56 pKa = 11.84AKK58 pKa = 9.48AVKK61 pKa = 10.03RR62 pKa = 11.84IFPRR66 pKa = 11.84AHH68 pKa = 6.54IEE70 pKa = 3.78IARR73 pKa = 11.84DD74 pKa = 3.06RR75 pKa = 11.84AALAKK80 pKa = 10.06YY81 pKa = 9.73VSKK84 pKa = 11.25EE85 pKa = 3.76EE86 pKa = 4.18TRR88 pKa = 11.84VATYY92 pKa = 8.37EE93 pKa = 4.13ANGVPSLFEE102 pKa = 3.95YY103 pKa = 10.5QDD105 pKa = 3.78NIAALWDD112 pKa = 3.51VEE114 pKa = 4.29EE115 pKa = 4.47FGRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84SDD122 pKa = 3.14DD123 pKa = 3.92AYY125 pKa = 11.11LKK127 pKa = 10.22RR128 pKa = 11.84YY129 pKa = 9.54KK130 pKa = 10.67YY131 pKa = 10.57DD132 pKa = 3.39VGDD135 pKa = 3.57LALAYY140 pKa = 10.29VDD142 pKa = 4.29EE143 pKa = 4.94LVASEE148 pKa = 4.13IVKK151 pKa = 9.43GQRR154 pKa = 11.84GIEE157 pKa = 4.24FIGINPMWRR166 pKa = 11.84SSWKK170 pKa = 10.02KK171 pKa = 9.26FYY173 pKa = 11.08SSIITRR179 pKa = 11.84HH180 pKa = 5.22ASSRR184 pKa = 11.84TQVQPSSAPSQASPPLSPGGDD205 pKa = 3.17QLDD208 pKa = 4.07QEE210 pKa = 5.55GEE212 pKa = 4.05LCDD215 pKa = 3.74DD216 pKa = 4.4HH217 pKa = 8.25GGSEE221 pKa = 4.03IDD223 pKa = 3.29

Molecular weight: 25.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9Q8|A0A126G9Q8_9VIRU Coat protein OS=Lake Sarah-associated circular virus-24 OX=1685751 PE=4 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84RR4 pKa = 11.84VRR6 pKa = 11.84KK7 pKa = 9.6SNRR10 pKa = 11.84PLRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 9.42LVRR19 pKa = 11.84RR20 pKa = 11.84SAQGGTSSIKK30 pKa = 9.28KK31 pKa = 9.99ANYY34 pKa = 8.21ATITEE39 pKa = 4.52VVKK42 pKa = 9.95LTDD45 pKa = 3.58FTLNTDD51 pKa = 2.84VNYY54 pKa = 10.92AFNLGEE60 pKa = 3.85FARR63 pKa = 11.84AKK65 pKa = 10.05EE66 pKa = 3.79LSNNFKK72 pKa = 10.62FYY74 pKa = 10.19RR75 pKa = 11.84AKK77 pKa = 9.62KK78 pKa = 7.77AHH80 pKa = 5.85YY81 pKa = 10.09RR82 pKa = 11.84FIPVSNVYY90 pKa = 9.95QSNGTSTSIAMPQFQMIMNRR110 pKa = 11.84TGDD113 pKa = 3.63NAVWTPAEE121 pKa = 4.12YY122 pKa = 10.25DD123 pKa = 3.38AQGAMAEE130 pKa = 4.42SFTRR134 pKa = 11.84TKK136 pKa = 9.18TFKK139 pKa = 10.74YY140 pKa = 10.25KK141 pKa = 10.41PNLVQAVQVQADD153 pKa = 4.15RR154 pKa = 11.84AAAPTIPIPLPFALASSQLRR174 pKa = 11.84QVGTRR179 pKa = 11.84PLYY182 pKa = 10.12DD183 pKa = 3.11AWLACPFSTIQTLGGIADD201 pKa = 4.38PNVDD205 pKa = 2.25IYY207 pKa = 11.53GDD209 pKa = 3.51VRR211 pKa = 11.84GEE213 pKa = 3.59ILYY216 pKa = 9.14WGHH219 pKa = 6.31SVTITTPNGTGTACEE234 pKa = 4.34VYY236 pKa = 10.87LEE238 pKa = 4.43VEE240 pKa = 4.36WEE242 pKa = 4.53FKK244 pKa = 11.04DD245 pKa = 3.43PMYY248 pKa = 10.17RR249 pKa = 11.84TVPPSVSAPEE259 pKa = 3.94PLKK262 pKa = 10.39LTEE265 pKa = 4.43LAPLAGPLTSGASEE279 pKa = 4.0

Molecular weight: 30.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

502

223

279

251.0

28.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.761 ± 0.501

0.797 ± 0.063

4.98 ± 1.105

6.175 ± 1.2

3.586 ± 0.566

6.375 ± 0.507

1.195 ± 0.379

5.179 ± 0.695

5.179 ± 0.128

6.375 ± 0.629

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.793 ± 0.283

3.984 ± 0.819

6.574 ± 0.755

4.382 ± 0.348

6.773 ± 0.029

6.972 ± 0.412

7.57 ± 1.101

6.175 ± 0.503

1.992 ± 0.442

4.183 ± 0.093
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski