Vibrio phage BONAISHI

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385A1D9|A0A385A1D9_9CAUD Uncharacterized protein OS=Vibrio phage BONAISHI OX=2283011 PE=4 SV=1
MM1 pKa = 7.67IDD3 pKa = 3.82KK4 pKa = 10.48ILARR8 pKa = 11.84VRR10 pKa = 11.84GDD12 pKa = 3.06VLTTHH17 pKa = 7.27DD18 pKa = 3.63VNAAYY23 pKa = 9.33TEE25 pKa = 3.81IDD27 pKa = 3.46MVFIDD32 pKa = 3.96GNGIYY37 pKa = 10.16SCIKK41 pKa = 10.27DD42 pKa = 3.47VPEE45 pKa = 4.13NSNVSLSDD53 pKa = 3.32TEE55 pKa = 4.16YY56 pKa = 10.49WKK58 pKa = 10.53QLWTGGNSPGGPVSDD73 pKa = 3.03ATYY76 pKa = 10.59YY77 pKa = 10.55KK78 pKa = 10.54SHH80 pKa = 7.9DD81 pKa = 3.64PTQDD85 pKa = 3.26YY86 pKa = 11.3QKK88 pKa = 11.13GDD90 pKa = 4.06EE91 pKa = 4.47IFLASGFYY99 pKa = 9.71RR100 pKa = 11.84AKK102 pKa = 10.49SDD104 pKa = 3.78VPGASNAALTDD115 pKa = 3.33PTYY118 pKa = 10.7WEE120 pKa = 4.36NFYY123 pKa = 11.3DD124 pKa = 4.07PQTGLWILEE133 pKa = 4.2VSDD136 pKa = 5.25DD137 pKa = 4.39GYY139 pKa = 11.88LEE141 pKa = 4.4LTTSGEE147 pKa = 4.6DD148 pKa = 2.94IDD150 pKa = 5.67KK151 pKa = 11.29YY152 pKa = 11.06IVDD155 pKa = 3.64EE156 pKa = 4.85DD157 pKa = 4.79GYY159 pKa = 10.13LTLVLL164 pKa = 4.68

Molecular weight: 18.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385A125|A0A385A125_9CAUD Major capsid protein OS=Vibrio phage BONAISHI OX=2283011 PE=4 SV=1
MM1 pKa = 7.16AQSAKK6 pKa = 10.41KK7 pKa = 8.73KK8 pKa = 8.97TNTAPAVRR16 pKa = 11.84PLNEE20 pKa = 4.09KK21 pKa = 10.05SVQVHH26 pKa = 6.67LIKK29 pKa = 10.96LLMLLRR35 pKa = 11.84KK36 pKa = 10.32DD37 pKa = 3.3KK38 pKa = 10.23VTLSRR43 pKa = 11.84FLYY46 pKa = 9.06LTILDD51 pKa = 4.36RR52 pKa = 11.84FQDD55 pKa = 3.42TGVYY59 pKa = 10.04VYY61 pKa = 10.97EE62 pKa = 4.09LMAWYY67 pKa = 10.07QSYY70 pKa = 8.77TGEE73 pKa = 3.97KK74 pKa = 9.75LAYY77 pKa = 7.59QTVIAMLNRR86 pKa = 11.84MAKK89 pKa = 9.26EE90 pKa = 3.86NEE92 pKa = 4.17VVKK95 pKa = 11.07SKK97 pKa = 10.78DD98 pKa = 2.95GKK100 pKa = 7.73YY101 pKa = 8.09TVYY104 pKa = 10.67RR105 pKa = 11.84LSLPGRR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84LDD115 pKa = 3.48KK116 pKa = 10.64MNKK119 pKa = 9.78AYY121 pKa = 10.51

Molecular weight: 14.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

301

0

301

90071

43

2008

299.2

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.249 ± 0.139

0.938 ± 0.051

7.026 ± 0.096

6.633 ± 0.118

4.073 ± 0.073

6.133 ± 0.136

2.195 ± 0.068

6.82 ± 0.098

6.051 ± 0.132

8.356 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.062

5.239 ± 0.098

4.019 ± 0.1

3.207 ± 0.081

4.726 ± 0.098

6.565 ± 0.126

6.116 ± 0.136

6.518 ± 0.079

1.259 ± 0.051

4.001 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski