Ornithinicoccus hortensis
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3571 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A542YLS3|A0A542YLS3_9MICO Amino acid/amide ABC transporter ATP-binding protein 1 (HAAT family) OS=Ornithinicoccus hortensis OX=82346 GN=FB467_0093 PE=4 SV=1
MM1 pKa = 7.62 SSTAHH6 pKa = 6.73 HH7 pKa = 6.8 DD8 pKa = 3.36 QPTFEE13 pKa = 4.52 GRR15 pKa = 11.84 PLARR19 pKa = 11.84 PEE21 pKa = 5.5 DD22 pKa = 4.05 DD23 pKa = 6.52 LEE25 pKa = 4.48 DD26 pKa = 3.48 QGAGFDD32 pKa = 3.51 AATLVTRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 VLSVVGVGVGAAALAACGTDD61 pKa = 2.96 ATTSTSSSSSTSSADD76 pKa = 3.28 NGSATSGGASADD88 pKa = 3.71 SGTATSDD95 pKa = 2.75 GSTAAGTLEE104 pKa = 4.72 EE105 pKa = 4.96 IPEE108 pKa = 4.35 EE109 pKa = 4.33 TNGPYY114 pKa = 9.97 PADD117 pKa = 3.5 GTQDD121 pKa = 3.47 VNVLTEE127 pKa = 4.09 SGIVRR132 pKa = 11.84 QDD134 pKa = 2.53 ITTSLDD140 pKa = 3.19 GGSSVDD146 pKa = 4.06 GVALTMTFLVHH157 pKa = 7.6 DD158 pKa = 4.56 MVNDD162 pKa = 3.58 QPFEE166 pKa = 4.15 GVAVYY171 pKa = 9.59 AWQCDD176 pKa = 3.4 AEE178 pKa = 4.73 GRR180 pKa = 11.84 YY181 pKa = 10.61 SMYY184 pKa = 9.51 TQGVEE189 pKa = 4.01 DD190 pKa = 4.15 EE191 pKa = 4.2 TWLRR195 pKa = 11.84 GIQVADD201 pKa = 3.43 SDD203 pKa = 4.34 GMVSFSTIVPGCYY216 pKa = 9.3 SGRR219 pKa = 11.84 WPHH222 pKa = 6.02 IHH224 pKa = 6.47 FEE226 pKa = 4.28 VYY228 pKa = 10.33 PDD230 pKa = 3.28 VDD232 pKa = 3.57 SATDD236 pKa = 3.38 VGNVIATSQVAFPEE250 pKa = 5.17 DD251 pKa = 3.95 ILTEE255 pKa = 3.96 IYY257 pKa = 9.73 TRR259 pKa = 11.84 SEE261 pKa = 4.23 YY262 pKa = 10.97 AGSAEE267 pKa = 3.75 NMAAVGSVEE276 pKa = 3.65 QDD278 pKa = 3.25 MIFADD283 pKa = 4.59 SLDD286 pKa = 3.57 QQMPTISGDD295 pKa = 3.11 VDD297 pKa = 3.32 SGYY300 pKa = 10.44 IATLTVNVDD309 pKa = 3.4 TTTEE313 pKa = 3.73 ATGAGGGPGGGAGGPGGGGQPPSGGGPGGEE343 pKa = 4.32 PPSGEE348 pKa = 4.11 PPTNN352 pKa = 3.36
Molecular weight: 35.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.668
IPC_protein 3.681
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.617
Nozaki 3.783
DTASelect 4.024
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.808
Patrickios 1.176
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A542YNC8|A0A542YNC8_9MICO L-aminopeptidase/D-esterase-like protein OS=Ornithinicoccus hortensis OX=82346 GN=FB467_0690 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 VHH17 pKa = 5.99 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILANRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.8 GRR40 pKa = 11.84 SSLSAA45 pKa = 3.62
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.676
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.398
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3571
0
3571
1191262
29
2581
333.6
35.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.878 ± 0.052
0.663 ± 0.012
6.402 ± 0.034
6.107 ± 0.043
2.68 ± 0.025
9.669 ± 0.035
2.184 ± 0.022
3.374 ± 0.029
1.575 ± 0.028
10.421 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.835 ± 0.018
1.623 ± 0.021
5.863 ± 0.031
3.041 ± 0.025
7.563 ± 0.046
5.053 ± 0.025
6.236 ± 0.035
9.351 ± 0.037
1.559 ± 0.019
1.924 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here