Amino acid dipepetide frequency for Acinetobacter phage BS46

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.237AlaAla: 3.237 ± 0.427
0.711AlaCys: 0.711 ± 0.165
3.094AlaAsp: 3.094 ± 0.327
3.059AlaGlu: 3.059 ± 0.334
1.921AlaPhe: 1.921 ± 0.28
2.632AlaGly: 2.632 ± 0.363
0.64AlaHis: 0.64 ± 0.142
4.055AlaIle: 4.055 ± 0.348
4.41AlaLys: 4.41 ± 0.482
4.126AlaLeu: 4.126 ± 0.408
1.352AlaMet: 1.352 ± 0.245
2.561AlaAsn: 2.561 ± 0.332
1.138AlaPro: 1.138 ± 0.21
1.992AlaGln: 1.992 ± 0.289
2.276AlaArg: 2.276 ± 0.284
4.055AlaSer: 4.055 ± 0.429
3.165AlaThr: 3.165 ± 0.353
3.663AlaVal: 3.663 ± 0.414
0.64AlaTrp: 0.64 ± 0.139
2.561AlaTyr: 2.561 ± 0.307
0.0AlaXaa: 0.0 ± 0.0
Cys
0.569CysAla: 0.569 ± 0.158
0.249CysCys: 0.249 ± 0.114
0.818CysAsp: 0.818 ± 0.16
0.996CysGlu: 0.996 ± 0.204
0.782CysPhe: 0.782 ± 0.17
1.138CysGly: 1.138 ± 0.214
0.462CysHis: 0.462 ± 0.119
0.711CysIle: 0.711 ± 0.169
1.138CysLys: 1.138 ± 0.227
1.103CysLeu: 1.103 ± 0.185
0.462CysMet: 0.462 ± 0.133
0.711CysAsn: 0.711 ± 0.15
0.285CysPro: 0.285 ± 0.103
0.32CysGln: 0.32 ± 0.102
0.534CysArg: 0.534 ± 0.124
1.067CysSer: 1.067 ± 0.187
0.747CysThr: 0.747 ± 0.174
0.782CysVal: 0.782 ± 0.168
0.356CysTrp: 0.356 ± 0.13
0.818CysTyr: 0.818 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
3.735AspAla: 3.735 ± 0.366
1.174AspCys: 1.174 ± 0.233
4.624AspAsp: 4.624 ± 0.386
4.766AspGlu: 4.766 ± 0.37
3.237AspPhe: 3.237 ± 0.388
5.051AspGly: 5.051 ± 0.402
1.067AspHis: 1.067 ± 0.211
5.442AspIle: 5.442 ± 0.529
4.197AspLys: 4.197 ± 0.446
5.655AspLeu: 5.655 ± 0.392
1.601AspMet: 1.601 ± 0.217
3.948AspAsn: 3.948 ± 0.452
1.245AspPro: 1.245 ± 0.199
1.707AspGln: 1.707 ± 0.278
2.419AspArg: 2.419 ± 0.281
3.059AspSer: 3.059 ± 0.304
3.272AspThr: 3.272 ± 0.296
5.015AspVal: 5.015 ± 0.419
1.352AspTrp: 1.352 ± 0.229
3.521AspTyr: 3.521 ± 0.419
0.0AspXaa: 0.0 ± 0.0
Glu
4.019GluAla: 4.019 ± 0.414
1.067GluCys: 1.067 ± 0.211
5.086GluAsp: 5.086 ± 0.481
5.975GluGlu: 5.975 ± 0.522
3.13GluPhe: 3.13 ± 0.348
4.161GluGly: 4.161 ± 0.403
1.174GluHis: 1.174 ± 0.2
5.762GluIle: 5.762 ± 0.458
5.655GluLys: 5.655 ± 0.483
6.615GluLeu: 6.615 ± 0.413
2.098GluMet: 2.098 ± 0.286
4.41GluAsn: 4.41 ± 0.421
1.067GluPro: 1.067 ± 0.176
2.774GluGln: 2.774 ± 0.348
2.383GluArg: 2.383 ± 0.238
4.41GluSer: 4.41 ± 0.424
3.735GluThr: 3.735 ± 0.319
5.371GluVal: 5.371 ± 0.361
1.138GluTrp: 1.138 ± 0.213
4.375GluTyr: 4.375 ± 0.42
0.0GluXaa: 0.0 ± 0.0
Phe
1.992PheAla: 1.992 ± 0.251
0.711PheCys: 0.711 ± 0.149
3.806PheAsp: 3.806 ± 0.383
3.45PheGlu: 3.45 ± 0.348
1.707PhePhe: 1.707 ± 0.28
2.845PheGly: 2.845 ± 0.341
0.64PheHis: 0.64 ± 0.148
3.237PheIle: 3.237 ± 0.428
3.806PheLys: 3.806 ± 0.422
3.308PheLeu: 3.308 ± 0.31
0.996PheMet: 0.996 ± 0.185
3.023PheAsn: 3.023 ± 0.326
1.174PhePro: 1.174 ± 0.196
1.28PheGln: 1.28 ± 0.168
1.778PheArg: 1.778 ± 0.232
3.308PheSer: 3.308 ± 0.345
2.596PheThr: 2.596 ± 0.283
3.13PheVal: 3.13 ± 0.315
0.534PheTrp: 0.534 ± 0.147
2.454PheTyr: 2.454 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
2.347GlyAla: 2.347 ± 0.352
0.64GlyCys: 0.64 ± 0.156
4.553GlyAsp: 4.553 ± 0.479
4.304GlyGlu: 4.304 ± 0.353
2.952GlyPhe: 2.952 ± 0.235
4.268GlyGly: 4.268 ± 0.539
0.747GlyHis: 0.747 ± 0.161
4.055GlyIle: 4.055 ± 0.458
4.766GlyLys: 4.766 ± 0.363
4.837GlyLeu: 4.837 ± 0.487
1.458GlyMet: 1.458 ± 0.248
3.343GlyAsn: 3.343 ± 0.333
0.107GlyPro: 0.107 ± 0.066
1.387GlyGln: 1.387 ± 0.221
2.668GlyArg: 2.668 ± 0.367
4.41GlySer: 4.41 ± 0.455
3.948GlyThr: 3.948 ± 0.528
5.264GlyVal: 5.264 ± 0.471
1.245GlyTrp: 1.245 ± 0.213
3.557GlyTyr: 3.557 ± 0.403
0.0GlyXaa: 0.0 ± 0.0
His
0.711HisAla: 0.711 ± 0.17
0.213HisCys: 0.213 ± 0.091
0.854HisAsp: 0.854 ± 0.186
1.387HisGlu: 1.387 ± 0.255
0.925HisPhe: 0.925 ± 0.206
1.031HisGly: 1.031 ± 0.192
0.391HisHis: 0.391 ± 0.117
1.316HisIle: 1.316 ± 0.223
1.387HisLys: 1.387 ± 0.234
1.352HisLeu: 1.352 ± 0.206
0.356HisMet: 0.356 ± 0.095
1.458HisAsn: 1.458 ± 0.235
0.854HisPro: 0.854 ± 0.215
0.462HisGln: 0.462 ± 0.119
0.676HisArg: 0.676 ± 0.151
0.96HisSer: 0.96 ± 0.16
1.067HisThr: 1.067 ± 0.214
0.996HisVal: 0.996 ± 0.182
0.249HisTrp: 0.249 ± 0.094
0.711HisTyr: 0.711 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
3.308IleAla: 3.308 ± 0.459
0.782IleCys: 0.782 ± 0.16
5.086IleAsp: 5.086 ± 0.44
5.726IleGlu: 5.726 ± 0.372
2.703IlePhe: 2.703 ± 0.379
4.481IleGly: 4.481 ± 0.43
0.996IleHis: 0.996 ± 0.182
4.09IleIle: 4.09 ± 0.434
6.758IleLys: 6.758 ± 0.542
5.157IleLeu: 5.157 ± 0.441
1.565IleMet: 1.565 ± 0.246
4.73IleAsn: 4.73 ± 0.415
1.921IlePro: 1.921 ± 0.29
2.952IleGln: 2.952 ± 0.324
2.916IleArg: 2.916 ± 0.367
4.624IleSer: 4.624 ± 0.417
4.197IleThr: 4.197 ± 0.394
4.09IleVal: 4.09 ± 0.407
0.818IleTrp: 0.818 ± 0.162
3.023IleTyr: 3.023 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
4.375LysAla: 4.375 ± 0.422
0.747LysCys: 0.747 ± 0.151
4.553LysAsp: 4.553 ± 0.441
7.007LysGlu: 7.007 ± 0.471
3.521LysPhe: 3.521 ± 0.371
4.197LysGly: 4.197 ± 0.473
1.778LysHis: 1.778 ± 0.262
4.588LysIle: 4.588 ± 0.35
5.477LysLys: 5.477 ± 0.545
7.931LysLeu: 7.931 ± 0.575
2.739LysMet: 2.739 ± 0.365
4.553LysAsn: 4.553 ± 0.401
2.454LysPro: 2.454 ± 0.31
2.739LysGln: 2.739 ± 0.299
4.055LysArg: 4.055 ± 0.414
4.659LysSer: 4.659 ± 0.383
3.912LysThr: 3.912 ± 0.425
5.086LysVal: 5.086 ± 0.482
0.96LysTrp: 0.96 ± 0.185
3.699LysTyr: 3.699 ± 0.397
0.0LysXaa: 0.0 ± 0.0
Leu
4.944LeuAla: 4.944 ± 0.471
1.067LeuCys: 1.067 ± 0.244
5.299LeuAsp: 5.299 ± 0.405
6.829LeuGlu: 6.829 ± 0.438
4.197LeuPhe: 4.197 ± 0.447
4.232LeuGly: 4.232 ± 0.346
1.423LeuHis: 1.423 ± 0.224
6.046LeuIle: 6.046 ± 0.54
7.433LeuLys: 7.433 ± 0.529
6.687LeuLeu: 6.687 ± 0.408
1.672LeuMet: 1.672 ± 0.282
5.726LeuAsn: 5.726 ± 0.425
2.668LeuPro: 2.668 ± 0.346
3.023LeuGln: 3.023 ± 0.389
2.347LeuArg: 2.347 ± 0.345
6.011LeuSer: 6.011 ± 0.432
5.477LeuThr: 5.477 ± 0.409
5.086LeuVal: 5.086 ± 0.414
0.889LeuTrp: 0.889 ± 0.162
3.308LeuTyr: 3.308 ± 0.3
0.0LeuXaa: 0.0 ± 0.0
Met
1.352MetAla: 1.352 ± 0.225
0.32MetCys: 0.32 ± 0.126
0.889MetAsp: 0.889 ± 0.19
1.067MetGlu: 1.067 ± 0.194
0.996MetPhe: 0.996 ± 0.211
0.925MetGly: 0.925 ± 0.168
0.213MetHis: 0.213 ± 0.088
1.992MetIle: 1.992 ± 0.26
2.525MetLys: 2.525 ± 0.285
2.525MetLeu: 2.525 ± 0.305
0.462MetMet: 0.462 ± 0.136
1.28MetAsn: 1.28 ± 0.239
0.462MetPro: 0.462 ± 0.12
1.031MetGln: 1.031 ± 0.187
0.925MetArg: 0.925 ± 0.187
2.454MetSer: 2.454 ± 0.265
1.743MetThr: 1.743 ± 0.237
1.423MetVal: 1.423 ± 0.254
0.249MetTrp: 0.249 ± 0.112
0.818MetTyr: 0.818 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
2.312AsnAla: 2.312 ± 0.231
0.747AsnCys: 0.747 ± 0.158
3.877AsnAsp: 3.877 ± 0.361
3.841AsnGlu: 3.841 ± 0.385
2.596AsnPhe: 2.596 ± 0.35
4.268AsnGly: 4.268 ± 0.458
1.031AsnHis: 1.031 ± 0.189
4.232AsnIle: 4.232 ± 0.332
4.695AsnLys: 4.695 ± 0.415
5.477AsnLeu: 5.477 ± 0.43
1.423AsnMet: 1.423 ± 0.198
3.699AsnAsn: 3.699 ± 0.39
2.098AsnPro: 2.098 ± 0.36
2.134AsnGln: 2.134 ± 0.21
2.419AsnArg: 2.419 ± 0.288
4.161AsnSer: 4.161 ± 0.371
3.699AsnThr: 3.699 ± 0.403
3.272AsnVal: 3.272 ± 0.319
0.356AsnTrp: 0.356 ± 0.109
2.952AsnTyr: 2.952 ± 0.351
0.0AsnXaa: 0.0 ± 0.0
Pro
1.387ProAla: 1.387 ± 0.255
0.534ProCys: 0.534 ± 0.159
1.778ProAsp: 1.778 ± 0.272
1.849ProGlu: 1.849 ± 0.27
1.174ProPhe: 1.174 ± 0.194
0.036ProGly: 0.036 ± 0.034
0.498ProHis: 0.498 ± 0.136
2.205ProIle: 2.205 ± 0.297
1.743ProLys: 1.743 ± 0.264
1.778ProLeu: 1.778 ± 0.23
0.462ProMet: 0.462 ± 0.105
1.601ProAsn: 1.601 ± 0.244
0.605ProPro: 0.605 ± 0.216
0.96ProGln: 0.96 ± 0.202
1.174ProArg: 1.174 ± 0.179
1.707ProSer: 1.707 ± 0.272
1.921ProThr: 1.921 ± 0.231
1.565ProVal: 1.565 ± 0.218
0.071ProTrp: 0.071 ± 0.047
1.601ProTyr: 1.601 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
2.632GlnAla: 2.632 ± 0.309
0.462GlnCys: 0.462 ± 0.14
1.921GlnAsp: 1.921 ± 0.261
1.849GlnGlu: 1.849 ± 0.25
1.458GlnPhe: 1.458 ± 0.234
2.205GlnGly: 2.205 ± 0.389
0.747GlnHis: 0.747 ± 0.169
2.098GlnIle: 2.098 ± 0.26
2.063GlnLys: 2.063 ± 0.278
2.916GlnLeu: 2.916 ± 0.31
0.996GlnMet: 0.996 ± 0.218
1.458GlnAsn: 1.458 ± 0.227
0.782GlnPro: 0.782 ± 0.136
1.672GlnGln: 1.672 ± 0.253
1.245GlnArg: 1.245 ± 0.189
2.347GlnSer: 2.347 ± 0.294
1.672GlnThr: 1.672 ± 0.238
2.952GlnVal: 2.952 ± 0.32
0.498GlnTrp: 0.498 ± 0.138
1.352GlnTyr: 1.352 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
1.956ArgAla: 1.956 ± 0.319
0.711ArgCys: 0.711 ± 0.196
2.098ArgAsp: 2.098 ± 0.247
2.988ArgGlu: 2.988 ± 0.347
1.814ArgPhe: 1.814 ± 0.3
2.596ArgGly: 2.596 ± 0.313
0.782ArgHis: 0.782 ± 0.155
3.094ArgIle: 3.094 ± 0.289
3.308ArgLys: 3.308 ± 0.366
3.983ArgLeu: 3.983 ± 0.454
0.889ArgMet: 0.889 ± 0.175
2.17ArgAsn: 2.17 ± 0.274
0.996ArgPro: 0.996 ± 0.182
1.031ArgGln: 1.031 ± 0.228
1.423ArgArg: 1.423 ± 0.201
2.276ArgSer: 2.276 ± 0.295
2.241ArgThr: 2.241 ± 0.257
2.596ArgVal: 2.596 ± 0.271
0.782ArgTrp: 0.782 ± 0.172
1.992ArgTyr: 1.992 ± 0.259
0.0ArgXaa: 0.0 ± 0.0
Ser
2.988SerAla: 2.988 ± 0.311
1.031SerCys: 1.031 ± 0.207
4.09SerAsp: 4.09 ± 0.337
5.691SerGlu: 5.691 ± 0.459
3.663SerPhe: 3.663 ± 0.382
4.481SerGly: 4.481 ± 0.475
1.174SerHis: 1.174 ± 0.217
4.588SerIle: 4.588 ± 0.375
5.051SerLys: 5.051 ± 0.369
6.295SerLeu: 6.295 ± 0.529
1.636SerMet: 1.636 ± 0.242
3.983SerAsn: 3.983 ± 0.362
1.636SerPro: 1.636 ± 0.261
2.276SerGln: 2.276 ± 0.259
2.596SerArg: 2.596 ± 0.349
4.766SerSer: 4.766 ± 0.522
3.308SerThr: 3.308 ± 0.354
4.873SerVal: 4.873 ± 0.466
0.782SerTrp: 0.782 ± 0.158
2.845SerTyr: 2.845 ± 0.319
0.0SerXaa: 0.0 ± 0.0
Thr
3.592ThrAla: 3.592 ± 0.402
0.64ThrCys: 0.64 ± 0.164
3.77ThrAsp: 3.77 ± 0.461
3.877ThrGlu: 3.877 ± 0.351
2.668ThrPhe: 2.668 ± 0.332
4.161ThrGly: 4.161 ± 0.531
1.352ThrHis: 1.352 ± 0.254
4.161ThrIle: 4.161 ± 0.388
4.588ThrLys: 4.588 ± 0.432
4.197ThrLeu: 4.197 ± 0.367
0.782ThrMet: 0.782 ± 0.16
2.916ThrAsn: 2.916 ± 0.338
2.134ThrPro: 2.134 ± 0.253
1.707ThrGln: 1.707 ± 0.22
2.49ThrArg: 2.49 ± 0.336
4.161ThrSer: 4.161 ± 0.376
3.628ThrThr: 3.628 ± 0.441
3.379ThrVal: 3.379 ± 0.293
0.747ThrTrp: 0.747 ± 0.16
2.49ThrTyr: 2.49 ± 0.29
0.0ThrXaa: 0.0 ± 0.0
Val
3.521ValAla: 3.521 ± 0.316
0.818ValCys: 0.818 ± 0.185
5.513ValAsp: 5.513 ± 0.498
5.975ValGlu: 5.975 ± 0.45
3.237ValPhe: 3.237 ± 0.346
4.517ValGly: 4.517 ± 0.464
1.138ValHis: 1.138 ± 0.225
4.481ValIle: 4.481 ± 0.39
5.371ValLys: 5.371 ± 0.44
4.979ValLeu: 4.979 ± 0.457
1.352ValMet: 1.352 ± 0.253
4.055ValAsn: 4.055 ± 0.354
1.707ValPro: 1.707 ± 0.193
1.743ValGln: 1.743 ± 0.244
3.023ValArg: 3.023 ± 0.335
5.193ValSer: 5.193 ± 0.387
3.557ValThr: 3.557 ± 0.364
5.691ValVal: 5.691 ± 0.517
0.711ValTrp: 0.711 ± 0.155
2.952ValTyr: 2.952 ± 0.301
0.0ValXaa: 0.0 ± 0.0
Trp
0.462TrpAla: 0.462 ± 0.117
0.213TrpCys: 0.213 ± 0.083
0.854TrpAsp: 0.854 ± 0.182
0.711TrpGlu: 0.711 ± 0.177
0.925TrpPhe: 0.925 ± 0.187
0.356TrpGly: 0.356 ± 0.112
0.213TrpHis: 0.213 ± 0.079
1.067TrpIle: 1.067 ± 0.243
0.818TrpLys: 0.818 ± 0.156
1.672TrpLeu: 1.672 ± 0.26
0.32TrpMet: 0.32 ± 0.104
0.96TrpAsn: 0.96 ± 0.16
0.0TrpPro: 0.0 ± 0.0
0.391TrpGln: 0.391 ± 0.127
0.996TrpArg: 0.996 ± 0.174
0.889TrpSer: 0.889 ± 0.19
0.569TrpThr: 0.569 ± 0.147
1.103TrpVal: 1.103 ± 0.204
0.427TrpTrp: 0.427 ± 0.128
0.534TrpTyr: 0.534 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.849TyrAla: 1.849 ± 0.264
1.209TyrCys: 1.209 ± 0.216
3.521TyrAsp: 3.521 ± 0.38
3.059TyrGlu: 3.059 ± 0.328
2.17TyrPhe: 2.17 ± 0.3
3.272TyrGly: 3.272 ± 0.389
0.889TyrHis: 0.889 ± 0.194
2.703TyrIle: 2.703 ± 0.385
3.841TyrLys: 3.841 ± 0.417
3.77TyrLeu: 3.77 ± 0.368
0.996TyrMet: 0.996 ± 0.19
2.703TyrAsn: 2.703 ± 0.306
1.316TyrPro: 1.316 ± 0.23
1.743TyrGln: 1.743 ± 0.291
1.387TyrArg: 1.387 ± 0.216
3.272TyrSer: 3.272 ± 0.323
2.988TyrThr: 2.988 ± 0.341
4.197TyrVal: 4.197 ± 0.44
0.676TyrTrp: 0.676 ± 0.133
2.454TyrTyr: 2.454 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 176 proteins (28117 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski