Amino acid dipepetide frequency for Actinobacteria bacterium IMCC26256

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.332AlaAla: 14.332 ± 0.194	1.056AlaCys: 1.056 ± 0.039	6.312AlaAsp: 6.312 ± 0.094	7.199AlaGlu: 7.199 ± 0.111	3.817AlaPhe: 3.817 ± 0.081	10.101AlaGly: 10.101 ± 0.13	2.045AlaHis: 2.045 ± 0.054	6.532AlaIle: 6.532 ± 0.099	3.394AlaLys: 3.394 ± 0.086	13.007AlaLeu: 13.007 ± 0.149	2.509AlaMet: 2.509 ± 0.058	2.937AlaAsn: 2.937 ± 0.084	5.45AlaPro: 5.45 ± 0.105	3.151AlaGln: 3.151 ± 0.064	7.612AlaArg: 7.612 ± 0.106	7.625AlaSer: 7.625 ± 0.123	6.654AlaThr: 6.654 ± 0.122	9.113AlaVal: 9.113 ± 0.107	1.546AlaTrp: 1.546 ± 0.056	2.215AlaTyr: 2.215 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.092CysAla: 1.092 ± 0.041	0.086CysCys: 0.086 ± 0.011	0.606CysAsp: 0.606 ± 0.03	0.552CysGlu: 0.552 ± 0.03	0.327CysPhe: 0.327 ± 0.019	0.935CysGly: 0.935 ± 0.043	0.176CysHis: 0.176 ± 0.016	0.426CysIle: 0.426 ± 0.027	0.199CysLys: 0.199 ± 0.017	0.677CysLeu: 0.677 ± 0.035	0.137CysMet: 0.137 ± 0.013	0.202CysAsn: 0.202 ± 0.017	0.459CysPro: 0.459 ± 0.023	0.213CysGln: 0.213 ± 0.019	0.506CysArg: 0.506 ± 0.027	0.686CysSer: 0.686 ± 0.032	0.494CysThr: 0.494 ± 0.035	0.737CysVal: 0.737 ± 0.038	0.227CysTrp: 0.227 ± 0.035	0.17CysTyr: 0.17 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	7.282AspAla: 7.282 ± 0.112	0.512AspCys: 0.512 ± 0.023	3.379AspAsp: 3.379 ± 0.08	4.112AspGlu: 4.112 ± 0.078	1.869AspPhe: 1.869 ± 0.047	5.637AspGly: 5.637 ± 0.112	1.206AspHis: 1.206 ± 0.041	2.543AspIle: 2.543 ± 0.057	1.158AspLys: 1.158 ± 0.045	6.423AspLeu: 6.423 ± 0.099	0.865AspMet: 0.865 ± 0.036	1.023AspAsn: 1.023 ± 0.041	3.812AspPro: 3.812 ± 0.076	1.527AspGln: 1.527 ± 0.044	3.944AspArg: 3.944 ± 0.072	3.514AspSer: 3.514 ± 0.076	2.456AspThr: 2.456 ± 0.063	4.385AspVal: 4.385 ± 0.077	0.834AspTrp: 0.834 ± 0.041	1.158AspTyr: 1.158 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.439GluAla: 7.439 ± 0.119	0.462GluCys: 0.462 ± 0.025	2.776GluAsp: 2.776 ± 0.06	3.813GluGlu: 3.813 ± 0.092	2.134GluPhe: 2.134 ± 0.05	4.448GluGly: 4.448 ± 0.081	1.456GluHis: 1.456 ± 0.048	4.303GluIle: 4.303 ± 0.076	1.759GluLys: 1.759 ± 0.059	5.725GluLeu: 5.725 ± 0.094	1.53GluMet: 1.53 ± 0.051	1.523GluAsn: 1.523 ± 0.041	2.706GluPro: 2.706 ± 0.058	2.1GluGln: 2.1 ± 0.056	5.463GluArg: 5.463 ± 0.099	3.994GluSer: 3.994 ± 0.072	3.469GluThr: 3.469 ± 0.069	5.458GluVal: 5.458 ± 0.104	0.983GluTrp: 0.983 ± 0.036	1.283GluTyr: 1.283 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	4.06PheAla: 4.06 ± 0.079	0.37PheCys: 0.37 ± 0.02	2.357PheAsp: 2.357 ± 0.047	2.146PheGlu: 2.146 ± 0.062	1.11PhePhe: 1.11 ± 0.046	3.449PheGly: 3.449 ± 0.067	0.587PheHis: 0.587 ± 0.026	1.604PheIle: 1.604 ± 0.053	0.874PheLys: 0.874 ± 0.038	2.909PheLeu: 2.909 ± 0.079	0.601PheMet: 0.601 ± 0.029	0.912PheAsn: 0.912 ± 0.035	1.567PhePro: 1.567 ± 0.046	0.726PheGln: 0.726 ± 0.033	1.873PheArg: 1.873 ± 0.054	2.159PheSer: 2.159 ± 0.056	1.919PheThr: 1.919 ± 0.052	2.778PheVal: 2.778 ± 0.06	0.396PheTrp: 0.396 ± 0.026	0.705PheTyr: 0.705 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	10.199GlyAla: 10.199 ± 0.197	0.81GlyCys: 0.81 ± 0.038	5.038GlyAsp: 5.038 ± 0.094	5.383GlyGlu: 5.383 ± 0.093	3.285GlyPhe: 3.285 ± 0.071	7.597GlyGly: 7.597 ± 0.101	1.618GlyHis: 1.618 ± 0.047	4.946GlyIle: 4.946 ± 0.093	2.545GlyLys: 2.545 ± 0.063	8.413GlyLeu: 8.413 ± 0.123	1.957GlyMet: 1.957 ± 0.054	2.079GlyAsn: 2.079 ± 0.069	3.829GlyPro: 3.829 ± 0.094	2.17GlyGln: 2.17 ± 0.058	5.747GlyArg: 5.747 ± 0.092	6.14GlySer: 6.14 ± 0.119	4.98GlyThr: 4.98 ± 0.11	8.186GlyVal: 8.186 ± 0.11	1.503GlyTrp: 1.503 ± 0.054	2.192GlyTyr: 2.192 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.944HisAla: 1.944 ± 0.057	0.206HisCys: 0.206 ± 0.017	1.172HisAsp: 1.172 ± 0.045	1.177HisGlu: 1.177 ± 0.038	0.661HisPhe: 0.661 ± 0.03	1.812HisGly: 1.812 ± 0.054	0.601HisHis: 0.601 ± 0.03	0.894HisIle: 0.894 ± 0.037	0.42HisLys: 0.42 ± 0.023	2.148HisLeu: 2.148 ± 0.058	0.356HisMet: 0.356 ± 0.02	0.408HisAsn: 0.408 ± 0.025	1.276HisPro: 1.276 ± 0.038	0.509HisGln: 0.509 ± 0.028	1.418HisArg: 1.418 ± 0.042	1.193HisSer: 1.193 ± 0.04	0.96HisThr: 0.96 ± 0.037	1.443HisVal: 1.443 ± 0.048	0.282HisTrp: 0.282 ± 0.018	0.441HisTyr: 0.441 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	7.535IleAla: 7.535 ± 0.113	0.558IleCys: 0.558 ± 0.029	3.991IleAsp: 3.991 ± 0.093	4.086IleGlu: 4.086 ± 0.085	1.576IlePhe: 1.576 ± 0.046	5.245IleGly: 5.245 ± 0.106	0.957IleHis: 0.957 ± 0.034	2.422IleIle: 2.422 ± 0.058	1.371IleLys: 1.371 ± 0.045	4.427IleLeu: 4.427 ± 0.097	0.775IleMet: 0.775 ± 0.033	1.481IleAsn: 1.481 ± 0.042	2.809IlePro: 2.809 ± 0.063	1.164IleGln: 1.164 ± 0.041	2.983IleArg: 2.983 ± 0.065	3.593IleSer: 3.593 ± 0.069	3.275IleThr: 3.275 ± 0.074	4.348IleVal: 4.348 ± 0.076	0.667IleTrp: 0.667 ± 0.032	1.011IleTyr: 1.011 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	3.189LysAla: 3.189 ± 0.085	0.24LysCys: 0.24 ± 0.028	1.336LysAsp: 1.336 ± 0.049	1.33LysGlu: 1.33 ± 0.047	0.846LysPhe: 0.846 ± 0.034	2.177LysGly: 2.177 ± 0.062	0.523LysHis: 0.523 ± 0.027	1.511LysIle: 1.511 ± 0.05	1.176LysLys: 1.176 ± 0.067	1.885LysLeu: 1.885 ± 0.055	0.66LysMet: 0.66 ± 0.029	0.792LysAsn: 0.792 ± 0.033	1.58LysPro: 1.58 ± 0.052	0.799LysGln: 0.799 ± 0.031	2.376LysArg: 2.376 ± 0.058	1.963LysSer: 1.963 ± 0.062	1.721LysThr: 1.721 ± 0.055	2.165LysVal: 2.165 ± 0.057	0.362LysTrp: 0.362 ± 0.021	0.663LysTyr: 0.663 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	12.139LeuAla: 12.139 ± 0.145	0.991LeuCys: 0.991 ± 0.04	6.107LeuAsp: 6.107 ± 0.106	6.146LeuGlu: 6.146 ± 0.108	2.879LeuPhe: 2.879 ± 0.073	9.164LeuGly: 9.164 ± 0.122	1.922LeuHis: 1.922 ± 0.052	4.954LeuIle: 4.954 ± 0.091	2.681LeuLys: 2.681 ± 0.068	9.938LeuLeu: 9.938 ± 0.159	1.845LeuMet: 1.845 ± 0.049	2.576LeuAsn: 2.576 ± 0.064	4.853LeuPro: 4.853 ± 0.078	2.319LeuGln: 2.319 ± 0.053	7.12LeuArg: 7.12 ± 0.109	6.355LeuSer: 6.355 ± 0.098	5.416LeuThr: 5.416 ± 0.087	8.242LeuVal: 8.242 ± 0.113	1.154LeuTrp: 1.154 ± 0.046	1.587LeuTyr: 1.587 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.239MetAla: 2.239 ± 0.054	0.153MetCys: 0.153 ± 0.015	0.871MetAsp: 0.871 ± 0.037	0.993MetGlu: 0.993 ± 0.035	0.618MetPhe: 0.618 ± 0.029	1.547MetGly: 1.547 ± 0.055	0.405MetHis: 0.405 ± 0.025	0.974MetIle: 0.974 ± 0.039	0.704MetLys: 0.704 ± 0.033	2.111MetLeu: 2.111 ± 0.058	0.383MetMet: 0.383 ± 0.021	0.694MetAsn: 0.694 ± 0.03	1.268MetPro: 1.268 ± 0.041	0.606MetGln: 0.606 ± 0.028	1.651MetArg: 1.651 ± 0.044	1.51MetSer: 1.51 ± 0.047	1.577MetThr: 1.577 ± 0.046	1.489MetVal: 1.489 ± 0.045	0.22MetTrp: 0.22 ± 0.017	0.33MetTyr: 0.33 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.202AsnAla: 3.202 ± 0.064	0.239AsnCys: 0.239 ± 0.016	1.36AsnAsp: 1.36 ± 0.044	1.433AsnGlu: 1.433 ± 0.041	0.845AsnPhe: 0.845 ± 0.035	2.582AsnGly: 2.582 ± 0.112	0.552AsnHis: 0.552 ± 0.025	1.206AsnIle: 1.206 ± 0.044	0.684AsnLys: 0.684 ± 0.027	2.66AsnLeu: 2.66 ± 0.077	0.43AsnMet: 0.43 ± 0.024	0.74AsnAsn: 0.74 ± 0.041	1.935AsnPro: 1.935 ± 0.053	0.729AsnGln: 0.729 ± 0.031	1.684AsnArg: 1.684 ± 0.057	1.704AsnSer: 1.704 ± 0.051	1.424AsnThr: 1.424 ± 0.048	1.922AsnVal: 1.922 ± 0.056	0.366AsnTrp: 0.366 ± 0.025	0.566AsnTyr: 0.566 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	5.174ProAla: 5.174 ± 0.091	0.304ProCys: 0.304 ± 0.022	3.359ProAsp: 3.359 ± 0.07	4.172ProGlu: 4.172 ± 0.087	1.748ProPhe: 1.748 ± 0.047	4.357ProGly: 4.357 ± 0.083	1.01ProHis: 1.01 ± 0.036	2.829ProIle: 2.829 ± 0.056	1.608ProLys: 1.608 ± 0.054	4.608ProLeu: 4.608 ± 0.064	1.06ProMet: 1.06 ± 0.039	1.667ProAsn: 1.667 ± 0.055	2.29ProPro: 2.29 ± 0.064	1.304ProGln: 1.304 ± 0.046	2.859ProArg: 2.859 ± 0.077	3.592ProSer: 3.592 ± 0.079	3.093ProThr: 3.093 ± 0.069	4.03ProVal: 4.03 ± 0.077	0.754ProTrp: 0.754 ± 0.036	1.041ProTyr: 1.041 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.788GlnAla: 2.788 ± 0.068	0.206GlnCys: 0.206 ± 0.017	1.084GlnAsp: 1.084 ± 0.04	1.261GlnGlu: 1.261 ± 0.041	0.816GlnPhe: 0.816 ± 0.034	2.14GlnGly: 2.14 ± 0.06	0.541GlnHis: 0.541 ± 0.029	1.687GlnIle: 1.687 ± 0.047	0.648GlnLys: 0.648 ± 0.034	2.554GlnLeu: 2.554 ± 0.065	0.685GlnMet: 0.685 ± 0.03	0.631GlnAsn: 0.631 ± 0.032	1.212GlnPro: 1.212 ± 0.04	0.814GlnGln: 0.814 ± 0.04	2.33GlnArg: 2.33 ± 0.061	1.7GlnSer: 1.7 ± 0.054	1.279GlnThr: 1.279 ± 0.045	2.371GlnVal: 2.371 ± 0.055	0.37GlnTrp: 0.37 ± 0.02	0.552GlnTyr: 0.552 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	6.978ArgAla: 6.978 ± 0.115	0.541ArgCys: 0.541 ± 0.032	4.234ArgAsp: 4.234 ± 0.076	4.212ArgGlu: 4.212 ± 0.084	2.606ArgPhe: 2.606 ± 0.062	5.032ArgGly: 5.032 ± 0.096	1.375ArgHis: 1.375 ± 0.048	3.97ArgIle: 3.97 ± 0.075	1.651ArgLys: 1.651 ± 0.053	7.101ArgLeu: 7.101 ± 0.094	1.602ArgMet: 1.602 ± 0.05	1.832ArgAsn: 1.832 ± 0.052	3.189ArgPro: 3.189 ± 0.073	1.588ArgGln: 1.588 ± 0.05	5.573ArgArg: 5.573 ± 0.094	5.241ArgSer: 5.241 ± 0.092	3.551ArgThr: 3.551 ± 0.068	6.004ArgVal: 6.004 ± 0.097	1.044ArgTrp: 1.044 ± 0.04	1.61ArgTyr: 1.61 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	7.353SerAla: 7.353 ± 0.114	0.5SerCys: 0.5 ± 0.034	3.956SerAsp: 3.956 ± 0.08	4.147SerGlu: 4.147 ± 0.079	2.179SerPhe: 2.179 ± 0.058	6.858SerGly: 6.858 ± 0.117	1.126SerHis: 1.126 ± 0.043	3.922SerIle: 3.922 ± 0.074	1.936SerLys: 1.936 ± 0.055	6.635SerLeu: 6.635 ± 0.105	1.51SerMet: 1.51 ± 0.043	2.142SerAsn: 2.142 ± 0.067	3.343SerPro: 3.343 ± 0.077	1.7SerGln: 1.7 ± 0.05	4.212SerArg: 4.212 ± 0.094	4.699SerSer: 4.699 ± 0.104	3.919SerThr: 3.919 ± 0.09	5.006SerVal: 5.006 ± 0.088	0.92SerTrp: 0.92 ± 0.038	1.226SerTyr: 1.226 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	6.027ThrAla: 6.027 ± 0.109	0.558ThrCys: 0.558 ± 0.034	3.074ThrAsp: 3.074 ± 0.064	3.151ThrGlu: 3.151 ± 0.07	1.862ThrPhe: 1.862 ± 0.047	5.618ThrGly: 5.618 ± 0.163	1.076ThrHis: 1.076 ± 0.033	3.053ThrIle: 3.053 ± 0.067	1.587ThrLys: 1.587 ± 0.046	5.4ThrLeu: 5.4 ± 0.099	1.03ThrMet: 1.03 ± 0.034	1.6ThrAsn: 1.6 ± 0.061	3.652ThrPro: 3.652 ± 0.078	1.588ThrGln: 1.588 ± 0.049	3.556ThrArg: 3.556 ± 0.076	3.72ThrSer: 3.72 ± 0.084	3.961ThrThr: 3.961 ± 0.119	4.38ThrVal: 4.38 ± 0.089	0.768ThrTrp: 0.768 ± 0.029	1.212ThrTyr: 1.212 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	10.136ValAla: 10.136 ± 0.124	0.701ValCys: 0.701 ± 0.033	4.75ValAsp: 4.75 ± 0.074	5.428ValGlu: 5.428 ± 0.097	2.598ValPhe: 2.598 ± 0.059	6.879ValGly: 6.879 ± 0.115	1.466ValHis: 1.466 ± 0.049	4.91ValIle: 4.91 ± 0.083	2.108ValLys: 2.108 ± 0.056	8.198ValLeu: 8.198 ± 0.112	1.732ValMet: 1.732 ± 0.051	2.179ValAsn: 2.179 ± 0.06	3.927ValPro: 3.927 ± 0.068	1.713ValGln: 1.713 ± 0.05	5.173ValArg: 5.173 ± 0.094	5.396ValSer: 5.396 ± 0.082	5.069ValThr: 5.069 ± 0.098	8.125ValVal: 8.125 ± 0.159	0.96ValTrp: 0.96 ± 0.039	1.309ValTyr: 1.309 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.307TrpAla: 1.307 ± 0.048	0.213TrpCys: 0.213 ± 0.02	0.685TrpAsp: 0.685 ± 0.03	0.677TrpGlu: 0.677 ± 0.03	0.554TrpPhe: 0.554 ± 0.028	1.201TrpGly: 1.201 ± 0.06	0.317TrpHis: 0.317 ± 0.022	0.85TrpIle: 0.85 ± 0.04	0.304TrpLys: 0.304 ± 0.022	1.377TrpLeu: 1.377 ± 0.046	0.355TrpMet: 0.355 ± 0.023	0.384TrpAsn: 0.384 ± 0.018	0.618TrpPro: 0.618 ± 0.032	0.395TrpGln: 0.395 ± 0.022	1.2TrpArg: 1.2 ± 0.046	1.204TrpSer: 1.204 ± 0.044	0.643TrpThr: 0.643 ± 0.03	1.052TrpVal: 1.052 ± 0.037	0.296TrpTrp: 0.296 ± 0.023	0.337TrpTyr: 0.337 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.19TyrAla: 2.19 ± 0.056	0.199TyrCys: 0.199 ± 0.016	1.191TyrAsp: 1.191 ± 0.042	1.407TyrGlu: 1.407 ± 0.049	0.772TyrPhe: 0.772 ± 0.032	1.84TyrGly: 1.84 ± 0.05	0.363TyrHis: 0.363 ± 0.027	0.739TyrIle: 0.739 ± 0.035	0.487TyrLys: 0.487 ± 0.029	2.194TyrLeu: 2.194 ± 0.063	0.319TyrMet: 0.319 ± 0.02	0.461TyrAsn: 0.461 ± 0.028	1.081TyrPro: 1.081 ± 0.042	0.552TyrGln: 0.552 ± 0.028	1.658TyrArg: 1.658 ± 0.05	1.303TyrSer: 1.303 ± 0.042	1.012TyrThr: 1.012 ± 0.036	1.525TyrVal: 1.525 ± 0.045	0.315TyrTrp: 0.315 ± 0.021	0.473TyrTyr: 0.473 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2299 proteins (757619 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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