Syntrophotalea acetylenivorans
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2762 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3GRU3|A0A1L3GRU3_9DELT Hydroxylamine reductase OS=Syntrophotalea acetylenivorans OX=1842532 GN=hcp PE=3 SV=1
MM1 pKa = 7.93 IITGLLIPAVALAEE15 pKa = 4.22 LKK17 pKa = 10.0 TGVVTVSPMLGGVLMEE33 pKa = 5.1 GDD35 pKa = 3.95 QPVDD39 pKa = 3.66 DD40 pKa = 5.73 DD41 pKa = 4.26 GVAYY45 pKa = 10.65 SLGLGYY51 pKa = 10.68 NLSKK55 pKa = 10.93 EE56 pKa = 4.33 FGLEE60 pKa = 3.85 AVLGGANLDD69 pKa = 4.07 SDD71 pKa = 4.83 DD72 pKa = 5.52 SSDD75 pKa = 3.79 SDD77 pKa = 3.41 VDD79 pKa = 3.96 LFTYY83 pKa = 10.61 RR84 pKa = 11.84 LDD86 pKa = 3.27 ALYY89 pKa = 11.01 RR90 pKa = 11.84 FMPDD94 pKa = 2.83 NKK96 pKa = 10.16 LVPYY100 pKa = 9.98 LAAGVGGYY108 pKa = 10.49 DD109 pKa = 4.92 LDD111 pKa = 5.19 NDD113 pKa = 4.54 HH114 pKa = 6.7 EE115 pKa = 4.44 WMANYY120 pKa = 10.38 GGGLLYY126 pKa = 10.33 FFAEE130 pKa = 4.65 NIALRR135 pKa = 11.84 ADD137 pKa = 3.61 VRR139 pKa = 11.84 HH140 pKa = 6.19 LMAFNEE146 pKa = 4.49 SNLEE150 pKa = 4.03 HH151 pKa = 6.86 NLTYY155 pKa = 10.03 TAGFLVQFAGVTAPAPKK172 pKa = 10.44 APLDD176 pKa = 3.7 SDD178 pKa = 3.91 GDD180 pKa = 4.3 GVTDD184 pKa = 6.62 DD185 pKa = 6.29 LDD187 pKa = 3.59 QCPNTPQGAPVDD199 pKa = 4.11 TKK201 pKa = 11.29 GCPLDD206 pKa = 3.72 SDD208 pKa = 4.0 GDD210 pKa = 4.31 GVFDD214 pKa = 5.93 YY215 pKa = 11.29 LDD217 pKa = 3.54 QCPDD221 pKa = 3.44 TPADD225 pKa = 3.59 APVDD229 pKa = 3.86 NKK231 pKa = 10.9 GCPLDD236 pKa = 3.8 SDD238 pKa = 4.0 GDD240 pKa = 4.31 GVFDD244 pKa = 5.93 YY245 pKa = 11.29 LDD247 pKa = 3.54 QCPDD251 pKa = 3.43 TPAGAPVDD259 pKa = 4.21 SKK261 pKa = 11.7 GCSLDD266 pKa = 3.35 SDD268 pKa = 3.96 GDD270 pKa = 3.9 GVYY273 pKa = 10.23 DD274 pKa = 5.54 YY275 pKa = 11.55 LDD277 pKa = 3.52 QCPGTPAGIPVDD289 pKa = 3.62 ATGCEE294 pKa = 4.02 LKK296 pKa = 10.02 LTLRR300 pKa = 11.84 INFDD304 pKa = 3.67 FNSAVIKK311 pKa = 10.56 PEE313 pKa = 3.74 FNAEE317 pKa = 3.43 VDD319 pKa = 3.39 KK320 pKa = 11.31 AAEE323 pKa = 3.91 FVRR326 pKa = 11.84 ANKK329 pKa = 9.58 NVPFILLTGHH339 pKa = 6.42 TDD341 pKa = 3.21 SMGKK345 pKa = 8.82 EE346 pKa = 4.22 AYY348 pKa = 9.46 NQQLSDD354 pKa = 3.31 QRR356 pKa = 11.84 AAAVRR361 pKa = 11.84 QALIDD366 pKa = 3.45 QYY368 pKa = 11.87 GFDD371 pKa = 4.01 GDD373 pKa = 3.93 KK374 pKa = 10.83 LVARR378 pKa = 11.84 GYY380 pKa = 11.52 GEE382 pKa = 4.13 TQPIADD388 pKa = 3.79 NSTEE392 pKa = 3.7 EE393 pKa = 3.75 GRR395 pKa = 11.84 YY396 pKa = 6.55 QNRR399 pKa = 11.84 RR400 pKa = 11.84 VEE402 pKa = 4.24 LVCCAVLPEE411 pKa = 4.05
Molecular weight: 43.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.795
IPC_protein 3.846
Toseland 3.605
ProMoST 4.012
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.668
Grimsley 3.516
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.668
EMBOSS 3.808
Sillero 3.973
Patrickios 1.43
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A1L3GR09|A0A1L3GR09_9DELT Transketolase OS=Syntrophotalea acetylenivorans OX=1842532 GN=A7E78_11150 PE=4 SV=1
MM1 pKa = 7.3 FFSLKK6 pKa = 9.98 IALSSLRR13 pKa = 11.84 AHH15 pKa = 7.57 RR16 pKa = 11.84 LRR18 pKa = 11.84 SILAMVGVFLGALALTGVQHH38 pKa = 6.5 VSRR41 pKa = 11.84 SMLLKK46 pKa = 10.6 AEE48 pKa = 4.47 HH49 pKa = 6.07 EE50 pKa = 4.33 TAKK53 pKa = 10.91 LGTNLFMVRR62 pKa = 11.84 SGQLSFRR69 pKa = 11.84 PSGSSRR75 pKa = 11.84 VRR77 pKa = 11.84 GLARR81 pKa = 11.84 NFTRR85 pKa = 11.84 QDD87 pKa = 3.02 AKK89 pKa = 11.36 ALLAGVPAARR99 pKa = 11.84 AGAPFVLATSPIRR112 pKa = 11.84 SGDD115 pKa = 3.65 LQINCQLVATLPSYY129 pKa = 10.02 ATVRR133 pKa = 11.84 NVKK136 pKa = 9.83 AAWGRR141 pKa = 11.84 FLSASDD147 pKa = 3.19 EE148 pKa = 4.18 AEE150 pKa = 3.93 RR151 pKa = 11.84 AKK153 pKa = 11.0 VCVLGASIARR163 pKa = 11.84 RR164 pKa = 11.84 LFGDD168 pKa = 3.39 GQGAVGQQVMLHH180 pKa = 5.86 RR181 pKa = 11.84 AGLRR185 pKa = 11.84 VIGVMEE191 pKa = 4.53 PKK193 pKa = 10.54 GADD196 pKa = 2.97 IAGTDD201 pKa = 3.01 QDD203 pKa = 3.78 EE204 pKa = 4.55 QVFVPLTTYY213 pKa = 8.62 MRR215 pKa = 11.84 RR216 pKa = 11.84 LANQDD221 pKa = 3.6 WISGVYY227 pKa = 9.66 LQLADD232 pKa = 3.53 SQAIEE237 pKa = 4.27 EE238 pKa = 4.36 ARR240 pKa = 11.84 QAAATILRR248 pKa = 11.84 RR249 pKa = 11.84 RR250 pKa = 11.84 HH251 pKa = 6.01 SIDD254 pKa = 3.12 VGEE257 pKa = 5.32 DD258 pKa = 3.24 DD259 pKa = 6.65 DD260 pKa = 4.47 FTLLAAQDD268 pKa = 3.61 TMRR271 pKa = 11.84 VQRR274 pKa = 11.84 DD275 pKa = 3.31 ALALVQTLGLLSSSISFAVGGLGILSIMVLLVRR308 pKa = 11.84 TRR310 pKa = 11.84 RR311 pKa = 11.84 LEE313 pKa = 3.78 IGVRR317 pKa = 11.84 RR318 pKa = 11.84 AVGAKK323 pKa = 9.67 RR324 pKa = 11.84 RR325 pKa = 11.84 DD326 pKa = 3.27 IVRR329 pKa = 11.84 QFMFEE334 pKa = 3.94 AGLMAGIGGGLGVLSSLLLVLVVCRR359 pKa = 11.84 IGEE362 pKa = 4.06 MPLAYY367 pKa = 10.26 DD368 pKa = 3.6 PVLIGSSLFGSLLLGLAAGAYY389 pKa = 7.53 PAWQAANVEE398 pKa = 4.14 ILQVLRR404 pKa = 11.84 NEE406 pKa = 4.23
Molecular weight: 43.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.428
IPC_protein 10.365
Toseland 10.526
ProMoST 10.306
Dawson 10.628
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.687
Grimsley 10.687
Solomon 10.774
Lehninger 10.745
Nozaki 10.526
DTASelect 10.379
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.57
Patrickios 10.379
IPC_peptide 10.789
IPC2_peptide 9.502
IPC2.peptide.svr19 8.69
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2762
0
2762
882519
33
1929
319.5
35.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.283 ± 0.053
1.322 ± 0.021
5.411 ± 0.035
6.661 ± 0.047
3.972 ± 0.031
7.79 ± 0.038
2.081 ± 0.02
5.619 ± 0.041
4.459 ± 0.039
11.513 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.022
3.127 ± 0.026
4.515 ± 0.029
4.253 ± 0.031
6.328 ± 0.043
5.692 ± 0.033
4.823 ± 0.029
7.145 ± 0.039
1.056 ± 0.017
2.641 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here