Circovirus-like genome CB-B

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GII9|C6GII9_9VIRU Putative Rep-associated protein OS=Circovirus-like genome CB-B OX=642257 PE=4 SV=1
MM1 pKa = 7.62LATPPNSPTVRR12 pKa = 11.84HH13 pKa = 5.65RR14 pKa = 11.84HH15 pKa = 5.12QGPAGDD21 pKa = 4.23SPDD24 pKa = 3.79NGVQALASGRR34 pKa = 11.84QAQEE38 pKa = 3.09QAAAFWVEE46 pKa = 3.94SSFPAGDD53 pKa = 3.35PRR55 pKa = 11.84RR56 pKa = 11.84AYY58 pKa = 9.93EE59 pKa = 3.9EE60 pKa = 3.82QRR62 pKa = 11.84SVGLCGEE69 pKa = 4.53GGDD72 pKa = 3.9FRR74 pKa = 11.84RR75 pKa = 11.84WDD77 pKa = 3.73EE78 pKa = 4.08LPAGEE83 pKa = 4.78GAKK86 pKa = 9.97QGSGLGPSEE95 pKa = 4.7GPSSEE100 pKa = 4.16GPVLGDD106 pKa = 4.14SLWHH110 pKa = 5.97AAQVRR115 pKa = 11.84RR116 pKa = 11.84LWEE119 pKa = 3.93MTDD122 pKa = 3.7VMWGDD127 pKa = 4.14LGMDD131 pKa = 4.09TDD133 pKa = 4.39PAHH136 pKa = 6.69

Molecular weight: 14.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GII8|C6GII8_9VIRU Rep-associated protein OS=Circovirus-like genome CB-B OX=642257 PE=4 SV=1
MM1 pKa = 7.77CIFPVSLLSKK11 pKa = 10.7KK12 pKa = 10.4NINCTMVVWPAVLPYY27 pKa = 10.57LEE29 pKa = 4.79AANTVAQGVQTANTIVHH46 pKa = 6.95EE47 pKa = 4.62GQQLAKK53 pKa = 9.47RR54 pKa = 11.84TFGTQISNKK63 pKa = 8.24NKK65 pKa = 9.46KK66 pKa = 9.24RR67 pKa = 11.84KK68 pKa = 9.59LDD70 pKa = 3.46VGHH73 pKa = 7.61ASTQTSGGGKK83 pKa = 9.57YY84 pKa = 9.29YY85 pKa = 9.92QAGYY89 pKa = 9.8VGRR92 pKa = 11.84FRR94 pKa = 11.84GKK96 pKa = 8.34RR97 pKa = 11.84AKK99 pKa = 9.43IVRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84IPSKK109 pKa = 10.51KK110 pKa = 9.32RR111 pKa = 11.84VAKK114 pKa = 10.23KK115 pKa = 9.59RR116 pKa = 11.84RR117 pKa = 11.84TYY119 pKa = 9.99KK120 pKa = 10.17RR121 pKa = 11.84KK122 pKa = 8.79SRR124 pKa = 11.84VVKK127 pKa = 10.53GEE129 pKa = 3.63NYY131 pKa = 8.67YY132 pKa = 11.27GRR134 pKa = 11.84VGSIATLEE142 pKa = 4.22VTGALSDD149 pKa = 3.78SDD151 pKa = 3.93CVYY154 pKa = 10.42IGHH157 pKa = 6.55SSFAVGQVMRR167 pKa = 11.84MLSVAIMRR175 pKa = 11.84KK176 pKa = 8.09LFKK179 pKa = 9.97MAISWEE185 pKa = 4.06PEE187 pKa = 4.01NISTNIPYY195 pKa = 10.44KK196 pKa = 10.59LVGGAQVSNGYY207 pKa = 7.05TLIVKK212 pKa = 9.99KK213 pKa = 10.71INTDD217 pKa = 3.89DD218 pKa = 3.66NVKK221 pKa = 8.73TQDD224 pKa = 3.64EE225 pKa = 5.16YY226 pKa = 10.9IGPANSHH233 pKa = 6.78NITAPADD240 pKa = 3.55WFRR243 pKa = 11.84TNHH246 pKa = 5.49MEE248 pKa = 5.19AISAQASSNTAVEE261 pKa = 4.3RR262 pKa = 11.84NNRR265 pKa = 11.84LLWLHH270 pKa = 6.17LVDD273 pKa = 3.73VGTGIVRR280 pKa = 11.84AQLDD284 pKa = 4.72LTTLMCDD291 pKa = 3.42VYY293 pKa = 11.33SKK295 pKa = 11.69SNLKK299 pKa = 9.04IQNVTVPSATATEE312 pKa = 4.04ADD314 pKa = 3.44NVNNVPLIGRR324 pKa = 11.84SYY326 pKa = 10.51HH327 pKa = 5.55ISNWQPMTADD337 pKa = 4.37DD338 pKa = 6.29DD339 pKa = 4.06MGPLNRR345 pKa = 11.84LNQDD349 pKa = 3.28TGVLGVEE356 pKa = 4.9IKK358 pKa = 10.65QSATLGSQYY367 pKa = 8.53VTWKK371 pKa = 10.25EE372 pKa = 4.0PPPSKK377 pKa = 10.97AFINCVSSSPEE388 pKa = 3.54RR389 pKa = 11.84MEE391 pKa = 4.65PGTIKK396 pKa = 10.61NHH398 pKa = 4.52VSILRR403 pKa = 11.84RR404 pKa = 11.84SMAFPRR410 pKa = 11.84LMEE413 pKa = 4.33ALAIRR418 pKa = 11.84RR419 pKa = 11.84GNVLNKK425 pKa = 9.66SVKK428 pKa = 9.88IGNHH432 pKa = 5.86DD433 pKa = 3.86LFAFEE438 pKa = 5.48RR439 pKa = 11.84MISVPGEE446 pKa = 3.99LPLKK450 pKa = 10.37IIYY453 pKa = 7.97EE454 pKa = 4.39CNIFTGCAVYY464 pKa = 10.41SRR466 pKa = 11.84KK467 pKa = 9.62KK468 pKa = 10.42AIMMQAVNSTAQGASTIPGG487 pKa = 3.36

Molecular weight: 53.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

789

136

487

263.0

28.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.492 ± 0.786

1.521 ± 0.324

3.802 ± 1.093

4.436 ± 0.944

2.535 ± 0.356

7.858 ± 1.883

2.155 ± 0.308

4.816 ± 1.938

6.084 ± 1.647

6.971 ± 0.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.788 ± 0.544

5.07 ± 1.622

6.464 ± 1.988

4.689 ± 0.724

5.703 ± 1.084

7.731 ± 0.172

6.337 ± 1.091

7.605 ± 1.002

2.155 ± 0.85

2.788 ± 0.657
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski