Ustilaginoidea virens RNA virus L
Average proteome isoelectric point is 7.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097I5E2|A0A097I5E2_9VIRU Coat protein OS=Ustilaginoidea virens RNA virus L OX=1561171 PE=4 SV=1
MM1 pKa = 8.37 SEE3 pKa = 3.82 INTNAFLAGILANARR18 pKa = 11.84 GGHH21 pKa = 5.8 IADD24 pKa = 3.5 NNTFRR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 9.45 ASSVRR36 pKa = 11.84 SSATIGGNEE45 pKa = 4.23 DD46 pKa = 3.12 ARR48 pKa = 11.84 LTRR51 pKa = 11.84 IFYY54 pKa = 10.34 EE55 pKa = 4.17 VGRR58 pKa = 11.84 VHH60 pKa = 7.34 ANARR64 pKa = 11.84 LALAAHH70 pKa = 7.49 ASTMLRR76 pKa = 11.84 IDD78 pKa = 3.28 ASYY81 pKa = 8.39 PTASVLAEE89 pKa = 4.09 EE90 pKa = 5.08 FVGLSKK96 pKa = 10.65 KK97 pKa = 10.43 YY98 pKa = 10.69 SNFAATFAYY107 pKa = 10.27 SSLAGVVEE115 pKa = 4.68 RR116 pKa = 11.84 VARR119 pKa = 11.84 GLAAASLFNDD129 pKa = 3.3 VDD131 pKa = 4.35 SDD133 pKa = 3.93 TLAANQPVRR142 pKa = 11.84 VSALGTYY149 pKa = 9.7 DD150 pKa = 3.74 GPVNSMVNTVFIPRR164 pKa = 11.84 LVNSTLTGDD173 pKa = 3.85 VFAVLVNAVAGEE185 pKa = 4.34 GGSVATDD192 pKa = 3.4 TLEE195 pKa = 3.97 VNAADD200 pKa = 4.04 RR201 pKa = 11.84 RR202 pKa = 11.84 PIIGTVDD209 pKa = 2.9 AVGFPRR215 pKa = 11.84 AAVDD219 pKa = 3.45 ALRR222 pKa = 11.84 LLGANMIASNQGPLFALALVRR243 pKa = 11.84 GLHH246 pKa = 6.02 KK247 pKa = 10.53 VLTVVGHH254 pKa = 5.36 TDD256 pKa = 2.77 EE257 pKa = 5.75 GGITRR262 pKa = 11.84 DD263 pKa = 3.4 LLRR266 pKa = 11.84 AQSFGVPFGGIHH278 pKa = 6.46 YY279 pKa = 10.43 GLDD282 pKa = 3.42 VYY284 pKa = 11.41 AGLPALATNSAADD297 pKa = 3.44 VASYY301 pKa = 11.3 VDD303 pKa = 4.04 SLALASAAAVAHH315 pKa = 6.8 CDD317 pKa = 3.02 PGTTYY322 pKa = 10.82 GGRR325 pKa = 11.84 WYY327 pKa = 8.55 PTFYY331 pKa = 10.69 CGASNGDD338 pKa = 3.2 AEE340 pKa = 4.78 TRR342 pKa = 11.84 SGTHH346 pKa = 6.01 LAGNDD351 pKa = 2.9 EE352 pKa = 4.15 MAVNNRR358 pKa = 11.84 SQLVADD364 pKa = 4.67 LAKK367 pKa = 10.69 FSDD370 pKa = 3.44 IFIPALARR378 pKa = 11.84 IFQCGGDD385 pKa = 3.7 PAVAASILTSSGHH398 pKa = 6.65 ALSKK402 pKa = 10.59 HH403 pKa = 4.57 NRR405 pKa = 11.84 HH406 pKa = 5.83 LRR408 pKa = 11.84 MATVTPFFWIEE419 pKa = 3.71 PTSILPHH426 pKa = 6.72 DD427 pKa = 4.41 AFGTRR432 pKa = 11.84 AEE434 pKa = 4.41 AEE436 pKa = 4.27 GSGALASRR444 pKa = 11.84 DD445 pKa = 3.59 QPRR448 pKa = 11.84 TKK450 pKa = 10.38 PIFEE454 pKa = 5.82 DD455 pKa = 2.58 IWTADD460 pKa = 3.28 TGDD463 pKa = 4.0 LAFSSYY469 pKa = 10.28 HH470 pKa = 4.57 VQLRR474 pKa = 11.84 NARR477 pKa = 11.84 SAWFLAHH484 pKa = 5.37 WHH486 pKa = 4.59 GHH488 pKa = 4.74 EE489 pKa = 4.53 LNGLGATQVRR499 pKa = 11.84 QLDD502 pKa = 3.99 PNAIIQPGGHH512 pKa = 4.81 QVEE515 pKa = 4.6 EE516 pKa = 3.93 VRR518 pKa = 11.84 DD519 pKa = 3.85 RR520 pKa = 11.84 VEE522 pKa = 3.81 SALPLSAYY530 pKa = 9.8 LWQRR534 pKa = 11.84 GQSPFCAPGEE544 pKa = 4.05 FMNLAGTMGIMVNHH558 pKa = 5.6 YY559 pKa = 8.86 TFDD562 pKa = 3.7 GDD564 pKa = 4.41 GIPRR568 pKa = 11.84 LQHH571 pKa = 6.31 LPTARR576 pKa = 11.84 EE577 pKa = 3.86 FLGATVTLTVGRR589 pKa = 11.84 PQGINDD595 pKa = 4.25 SKK597 pKa = 11.72 SNWADD602 pKa = 3.25 SQVRR606 pKa = 11.84 RR607 pKa = 11.84 ARR609 pKa = 11.84 TRR611 pKa = 11.84 AARR614 pKa = 11.84 EE615 pKa = 3.86 LAASAARR622 pKa = 11.84 VRR624 pKa = 11.84 AFGRR628 pKa = 11.84 PDD630 pKa = 3.17 VAAMPILTTAPPPRR644 pKa = 11.84 HH645 pKa = 5.88 AQGAQPVPARR655 pKa = 11.84 DD656 pKa = 3.46 HH657 pKa = 7.15 DD658 pKa = 4.18 VGGGVDD664 pKa = 3.26 GWRR667 pKa = 11.84 RR668 pKa = 11.84 TSVGVNDD675 pKa = 3.66 STTAYY680 pKa = 9.88 PYY682 pKa = 10.97 GEE684 pKa = 4.19 PLGVTAHH691 pKa = 5.9 HH692 pKa = 5.99 QPVRR696 pKa = 11.84 FPQLARR702 pKa = 11.84 TLASGGTAAVPPPPPAATSSDD723 pKa = 3.74 DD724 pKa = 3.49 VSPDD728 pKa = 3.2 VPVALSPSAAPLSGPTQSS746 pKa = 3.77
Molecular weight: 78.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.201
IPC2_protein 6.02
IPC_protein 6.135
Toseland 6.649
ProMoST 6.532
Dawson 6.465
Bjellqvist 6.453
Wikipedia 6.504
Rodwell 6.465
Grimsley 6.854
Solomon 6.478
Lehninger 6.465
Nozaki 6.751
DTASelect 6.956
Thurlkill 6.971
EMBOSS 6.942
Sillero 6.854
Patrickios 4.355
IPC_peptide 6.491
IPC2_peptide 6.854
IPC2.peptide.svr19 6.759
Protein with the highest isoelectric point:
>tr|A0A097I5E2|A0A097I5E2_9VIRU Coat protein OS=Ustilaginoidea virens RNA virus L OX=1561171 PE=4 SV=1
MM1 pKa = 8.2 DD2 pKa = 4.41 IYY4 pKa = 11.13 EE5 pKa = 4.22 RR6 pKa = 11.84 AKK8 pKa = 11.04 DD9 pKa = 3.94 FGPLGRR15 pKa = 11.84 VLIEE19 pKa = 5.07 HH20 pKa = 6.99 IEE22 pKa = 4.08 SCPDD26 pKa = 2.84 IVSDD30 pKa = 4.65 FRR32 pKa = 11.84 EE33 pKa = 4.16 TFIGRR38 pKa = 11.84 LIKK41 pKa = 9.73 LTNSANSLRR50 pKa = 11.84 TRR52 pKa = 11.84 SYY54 pKa = 10.87 LLPAAVSLLCLDD66 pKa = 5.04 FPLQEE71 pKa = 4.81 DD72 pKa = 3.76 MTFASFLRR80 pKa = 11.84 WVRR83 pKa = 11.84 EE84 pKa = 3.95 AYY86 pKa = 10.11 DD87 pKa = 3.98 LNIVDD92 pKa = 4.45 SPAFGEE98 pKa = 4.22 SDD100 pKa = 4.01 LAALPPGRR108 pKa = 11.84 LVRR111 pKa = 11.84 RR112 pKa = 11.84 LCRR115 pKa = 11.84 SKK117 pKa = 11.02 LRR119 pKa = 11.84 LASAFPEE126 pKa = 4.36 PEE128 pKa = 3.99 LLAGQTKK135 pKa = 10.46 PNMKK139 pKa = 9.96 LGPLISSSVIMMGSEE154 pKa = 3.57 RR155 pKa = 11.84 VAALLRR161 pKa = 11.84 SSWNMRR167 pKa = 11.84 SDD169 pKa = 3.26 RR170 pKa = 11.84 VMSKK174 pKa = 10.42 LVYY177 pKa = 10.62 AFVLSRR183 pKa = 11.84 RR184 pKa = 11.84 FGEE187 pKa = 4.44 VGHH190 pKa = 6.64 KK191 pKa = 9.63 LANFLVNNPDD201 pKa = 3.44 DD202 pKa = 4.0 AKK204 pKa = 11.14 YY205 pKa = 10.63 ISLALKK211 pKa = 10.63 ALGLNSTDD219 pKa = 3.07 WGSVLCEE226 pKa = 3.83 ANTLAGRR233 pKa = 11.84 GTGGIDD239 pKa = 3.62 LARR242 pKa = 11.84 EE243 pKa = 3.66 AAQRR247 pKa = 11.84 CDD249 pKa = 3.22 RR250 pKa = 11.84 DD251 pKa = 3.84 FVDD254 pKa = 3.14 SHH256 pKa = 6.9 IVHH259 pKa = 6.63 VDD261 pKa = 3.18 PDD263 pKa = 3.69 EE264 pKa = 4.44 LRR266 pKa = 11.84 PHH268 pKa = 6.52 VKK270 pKa = 10.36 SILDD274 pKa = 3.55 RR275 pKa = 11.84 EE276 pKa = 4.49 LKK278 pKa = 10.36 NIRR281 pKa = 11.84 SMAPLDD287 pKa = 4.52 DD288 pKa = 4.07 FWTSRR293 pKa = 11.84 WLWCVNGSQTTEE305 pKa = 3.44 SSLALGIDD313 pKa = 3.22 PHH315 pKa = 6.32 YY316 pKa = 11.15 CKK318 pKa = 9.89 ATHH321 pKa = 5.64 KK322 pKa = 10.03 RR323 pKa = 11.84 AYY325 pKa = 9.41 RR326 pKa = 11.84 RR327 pKa = 11.84 SASEE331 pKa = 3.76 HH332 pKa = 5.85 VKK334 pKa = 10.85 NEE336 pKa = 4.97 PITSWDD342 pKa = 2.86 GRR344 pKa = 11.84 TSVSVSAKK352 pKa = 10.53 LEE354 pKa = 4.25 AGKK357 pKa = 8.27 TRR359 pKa = 11.84 AIFACDD365 pKa = 3.1 TRR367 pKa = 11.84 SYY369 pKa = 11.08 FAFSWILSAAQRR381 pKa = 11.84 DD382 pKa = 3.9 WANSRR387 pKa = 11.84 VLLDD391 pKa = 4.05 PGVGGHH397 pKa = 7.09 LGMFQRR403 pKa = 11.84 ISRR406 pKa = 11.84 AQRR409 pKa = 11.84 GGGVNLMLDD418 pKa = 3.48 YY419 pKa = 11.52 DD420 pKa = 4.65 DD421 pKa = 6.31 FNSQHH426 pKa = 5.95 STSSMQVVFDD436 pKa = 4.26 EE437 pKa = 4.93 LCSKK441 pKa = 9.74 YY442 pKa = 10.67 NCPSWYY448 pKa = 9.74 RR449 pKa = 11.84 DD450 pKa = 3.36 TLVGSFEE457 pKa = 4.57 KK458 pKa = 10.59 MFVNCGNEE466 pKa = 3.92 RR467 pKa = 11.84 KK468 pKa = 9.35 RR469 pKa = 11.84 VLGTLMSGHH478 pKa = 7.18 RR479 pKa = 11.84 GTTFINSVLNAAYY492 pKa = 9.39 IRR494 pKa = 11.84 LAIGGSKK501 pKa = 9.97 FDD503 pKa = 4.37 RR504 pKa = 11.84 LLSLHH509 pKa = 6.53 TGDD512 pKa = 5.19 DD513 pKa = 3.71 VYY515 pKa = 11.35 IRR517 pKa = 11.84 ANTLADD523 pKa = 3.7 CVHH526 pKa = 6.88 ILDD529 pKa = 3.95 STKK532 pKa = 10.92 EE533 pKa = 3.88 LGCRR537 pKa = 11.84 MNPTKK542 pKa = 10.56 QSIGYY547 pKa = 9.19 KK548 pKa = 9.28 GAEE551 pKa = 4.25 FLRR554 pKa = 11.84 MGMRR558 pKa = 11.84 QDD560 pKa = 3.81 DD561 pKa = 4.06 CFGYY565 pKa = 7.96 FTRR568 pKa = 11.84 ALSSFVSGNWANTDD582 pKa = 3.19 SLDD585 pKa = 3.66 PVEE588 pKa = 4.84 GLRR591 pKa = 11.84 SAIGGCRR598 pKa = 11.84 SMINRR603 pKa = 11.84 GQVSAVAEE611 pKa = 4.22 LVGPAPRR618 pKa = 11.84 YY619 pKa = 9.79 RR620 pKa = 11.84 PGLGARR626 pKa = 11.84 TLCKK630 pKa = 9.61 MLKK633 pKa = 10.18 CEE635 pKa = 3.87 IAIVGSPVFGGSGFLTTYY653 pKa = 10.59 RR654 pKa = 11.84 VTTRR658 pKa = 11.84 VEE660 pKa = 3.39 DD661 pKa = 4.52 RR662 pKa = 11.84 IRR664 pKa = 11.84 VDD666 pKa = 3.46 KK667 pKa = 10.82 RR668 pKa = 11.84 WARR671 pKa = 11.84 YY672 pKa = 7.53 ATGDD676 pKa = 3.72 YY677 pKa = 10.0 LAHH680 pKa = 6.3 HH681 pKa = 6.09 TTSIEE686 pKa = 3.84 AAALEE691 pKa = 4.4 MSGSAGTNLLIISSYY706 pKa = 10.52 VRR708 pKa = 11.84 GLNEE712 pKa = 4.02 EE713 pKa = 4.19 RR714 pKa = 11.84 VQPKK718 pKa = 9.05 PAPSWKK724 pKa = 9.76 KK725 pKa = 10.13 LPRR728 pKa = 11.84 INLRR732 pKa = 11.84 GYY734 pKa = 10.51 ACASDD739 pKa = 4.39 LVNKK743 pKa = 8.18 QRR745 pKa = 11.84 KK746 pKa = 9.14 RR747 pKa = 11.84 GVLSHH752 pKa = 6.54 YY753 pKa = 10.41 PLINLIKK760 pKa = 10.51 NRR762 pKa = 11.84 LDD764 pKa = 3.27 EE765 pKa = 4.62 AMLRR769 pKa = 11.84 TLIAEE774 pKa = 4.06 EE775 pKa = 4.16 LGYY778 pKa = 10.82 VPEE781 pKa = 4.52 GEE783 pKa = 4.25 PYY785 pKa = 10.44 KK786 pKa = 10.55 IAFGEE791 pKa = 4.1 DD792 pKa = 3.57 SNSKK796 pKa = 10.36 RR797 pKa = 11.84 ILGVLSYY804 pKa = 11.28 ADD806 pKa = 3.34 ASAYY810 pKa = 10.19 CSRR813 pKa = 11.84 TDD815 pKa = 3.14 SGSVYY820 pKa = 8.51 TLCRR824 pKa = 11.84 VAMM827 pKa = 4.53
Molecular weight: 91.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.221
IPC2_protein 8.258
IPC_protein 8.097
Toseland 7.966
ProMoST 8.653
Dawson 8.873
Bjellqvist 9.092
Wikipedia 8.887
Rodwell 8.931
Grimsley 7.98
Solomon 8.931
Lehninger 8.916
Nozaki 9.253
DTASelect 8.858
Thurlkill 8.931
EMBOSS 9.048
Sillero 9.194
Patrickios 4.533
IPC_peptide 8.916
IPC2_peptide 8.097
IPC2.peptide.svr19 8.068
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1573
746
827
786.5
85.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.507 ± 2.256
1.526 ± 0.637
5.785 ± 0.1
3.878 ± 0.511
3.751 ± 0.088
8.328 ± 0.506
2.479 ± 0.388
4.132 ± 0.416
2.606 ± 1.073
9.599 ± 0.829
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.971 ± 0.405
4.069 ± 0.056
5.022 ± 0.822
2.225 ± 0.38
7.883 ± 0.587
8.455 ± 0.696
5.658 ± 0.672
6.993 ± 0.589
1.335 ± 0.169
2.797 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here