Microbacterium phage Appa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Appavirus; Microbacterium virus Appa

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UHT7|A0A2U8UHT7_9CAUD Uncharacterized protein OS=Microbacterium phage Appa OX=2182350 GN=11 PE=4 SV=1
MM1 pKa = 7.18TKK3 pKa = 10.04RR4 pKa = 11.84RR5 pKa = 11.84STIMTEE11 pKa = 3.82QTVNPLPAFNIDD23 pKa = 3.96DD24 pKa = 3.82VDD26 pKa = 4.51DD27 pKa = 4.96GEE29 pKa = 4.94RR30 pKa = 11.84NWAALALCVLDD41 pKa = 4.26IEE43 pKa = 4.64TRR45 pKa = 11.84RR46 pKa = 11.84CIYY49 pKa = 10.11CGQRR53 pKa = 11.84AVFDD57 pKa = 3.87TYY59 pKa = 11.73AEE61 pKa = 4.36YY62 pKa = 10.72VAHH65 pKa = 6.79FWAFEE70 pKa = 4.02YY71 pKa = 10.82EE72 pKa = 3.91GDD74 pKa = 3.84PWHH77 pKa = 6.96GPDD80 pKa = 5.69DD81 pKa = 4.24EE82 pKa = 6.99GDD84 pKa = 3.53FDD86 pKa = 4.49RR87 pKa = 11.84TFSLCLATADD97 pKa = 4.84DD98 pKa = 4.41VADD101 pKa = 5.09DD102 pKa = 4.52SPIAAPRR109 pKa = 11.84DD110 pKa = 3.59WNDD113 pKa = 2.85EE114 pKa = 3.69SRR116 pKa = 11.84TIVATYY122 pKa = 10.81ASTTGPGYY130 pKa = 10.87ALNVEE135 pKa = 4.83DD136 pKa = 5.16VDD138 pKa = 4.16SATYY142 pKa = 10.02RR143 pKa = 11.84PYY145 pKa = 11.14SRR147 pKa = 11.84TSAAVVRR154 pKa = 4.6

Molecular weight: 17.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UHW4|A0A2U8UHW4_9CAUD Fibronectin type-III domain-containing protein OS=Microbacterium phage Appa OX=2182350 GN=26 PE=4 SV=1
MM1 pKa = 6.97TVEE4 pKa = 5.56PIQVDD9 pKa = 3.89PDD11 pKa = 3.44AEE13 pKa = 4.18RR14 pKa = 11.84YY15 pKa = 5.79PTSIIICAWLLGGHH29 pKa = 6.51DD30 pKa = 3.57WRR32 pKa = 11.84LLGRR36 pKa = 11.84WDD38 pKa = 2.87WKK40 pKa = 8.15TRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84TIDD47 pKa = 2.97RR48 pKa = 11.84EE49 pKa = 3.76RR50 pKa = 11.84CTRR53 pKa = 11.84CGVIRR58 pKa = 11.84PRR60 pKa = 11.84GGFFGARR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84DD70 pKa = 3.3LALTHH75 pKa = 6.73

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12600

42

732

193.8

21.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.444 ± 0.372

0.492 ± 0.109

6.921 ± 0.235

6.508 ± 0.276

2.706 ± 0.216

8.421 ± 0.397

1.786 ± 0.201

4.746 ± 0.238

3.071 ± 0.218

7.579 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.2

2.429 ± 0.183

5.183 ± 0.363

3.19 ± 0.178

7.738 ± 0.489

5.69 ± 0.232

6.738 ± 0.29

7.73 ± 0.196

1.841 ± 0.17

2.254 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski