Gramella jeungdoensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gramella

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3093 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8ATS1|A0A4Y8ATS1_9FLAO Glycoside hydrolase family 95 protein OS=Gramella jeungdoensis OX=708091 GN=E2488_07180 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.15RR3 pKa = 11.84TLLSILLFISFIPFYY18 pKa = 11.02GQIQPFYY25 pKa = 10.93NDD27 pKa = 3.41LDD29 pKa = 3.87FTKK32 pKa = 10.47TEE34 pKa = 3.85NEE36 pKa = 3.92LFIEE40 pKa = 4.56LSNRR44 pKa = 11.84LIDD47 pKa = 3.39THH49 pKa = 5.62TAIPYY54 pKa = 7.79TSSSTDD60 pKa = 2.6VWDD63 pKa = 3.41VLKK66 pKa = 10.86QVDD69 pKa = 4.65EE70 pKa = 5.2DD71 pKa = 4.4PDD73 pKa = 3.65NASNVLLIYY82 pKa = 10.22GFNDD86 pKa = 2.91TDD88 pKa = 4.95GIVDD92 pKa = 3.69TDD94 pKa = 3.44RR95 pKa = 11.84SRR97 pKa = 11.84NKK99 pKa = 10.07NLQDD103 pKa = 3.92SGGGDD108 pKa = 3.2PGRR111 pKa = 11.84WNRR114 pKa = 11.84EE115 pKa = 3.51HH116 pKa = 7.36VFAKK120 pKa = 10.55SLANPSLEE128 pKa = 4.17TGEE131 pKa = 4.89PGPGTDD137 pKa = 3.44VHH139 pKa = 6.2NLKK142 pKa = 10.17PADD145 pKa = 3.67SEE147 pKa = 4.62RR148 pKa = 11.84NSDD151 pKa = 3.07RR152 pKa = 11.84SNRR155 pKa = 11.84KK156 pKa = 8.11FTDD159 pKa = 3.69GSGNSGIVSSNGGWYY174 pKa = 9.72PGDD177 pKa = 3.33EE178 pKa = 4.3WKK180 pKa = 11.21GDD182 pKa = 3.39VARR185 pKa = 11.84IIMYY189 pKa = 8.9MYY191 pKa = 10.58LRR193 pKa = 11.84YY194 pKa = 10.21NGTGAQISQTQCLPINSGIGEE215 pKa = 4.27ALANDD220 pKa = 4.43PNMIDD225 pKa = 6.02LFLNWNIDD233 pKa = 3.66DD234 pKa = 4.24PVSEE238 pKa = 5.39FEE240 pKa = 4.12NQRR243 pKa = 11.84NDD245 pKa = 3.55YY246 pKa = 10.88IEE248 pKa = 4.24TVQGNRR254 pKa = 11.84NPFIDD259 pKa = 3.96NPYY262 pKa = 10.19LATLIWGGVNAEE274 pKa = 4.92DD275 pKa = 4.28KK276 pKa = 10.73WNLNNSSDD284 pKa = 3.96EE285 pKa = 4.17EE286 pKa = 4.78APTSPTNLVALNTTFEE302 pKa = 4.29SLEE305 pKa = 4.32INWDD309 pKa = 3.4AATDD313 pKa = 3.99NIGVIDD319 pKa = 4.33YY320 pKa = 10.63IIYY323 pKa = 10.65LDD325 pKa = 3.77GKK327 pKa = 7.97YY328 pKa = 10.74LKK330 pKa = 8.04TTFSTSSTILEE341 pKa = 4.26LTPNTTYY348 pKa = 11.12NITVKK353 pKa = 10.72ARR355 pKa = 11.84DD356 pKa = 3.52AFSNLSEE363 pKa = 4.21VGTNLSVTTLEE374 pKa = 4.52GPTYY378 pKa = 10.54LINEE382 pKa = 4.45DD383 pKa = 4.6FSNCSNLAFFTYY395 pKa = 10.9NEE397 pKa = 4.1ASNKK401 pKa = 8.97NWEE404 pKa = 4.47CEE406 pKa = 4.06NQFGEE411 pKa = 4.5NNTGSIGINGHH422 pKa = 4.86QQDD425 pKa = 4.53VLSKK429 pKa = 10.17DD430 pKa = 3.17WLITSSPINFDD441 pKa = 3.33EE442 pKa = 4.71NEE444 pKa = 3.89AEE446 pKa = 4.53KK447 pKa = 10.67ISFYY451 pKa = 11.07TDD453 pKa = 2.63AAYY456 pKa = 10.47GNTPLEE462 pKa = 4.06IVYY465 pKa = 10.55SSNYY469 pKa = 9.75DD470 pKa = 3.14GSSNPGQNDD479 pKa = 3.75FTWTPMPNISIPLHH493 pKa = 6.01SNGGATEE500 pKa = 4.49EE501 pKa = 4.52IYY503 pKa = 11.1AFSNVDD509 pKa = 2.81ISSITGTVYY518 pKa = 10.41IAFKK522 pKa = 10.71YY523 pKa = 9.81YY524 pKa = 9.55ATSAPTRR531 pKa = 11.84WTVDD535 pKa = 2.92SFEE538 pKa = 5.81IIADD542 pKa = 3.82NNDD545 pKa = 4.54DD546 pKa = 3.68IDD548 pKa = 5.78GDD550 pKa = 4.36GILNVDD556 pKa = 4.63DD557 pKa = 4.61NCPTTSNTDD566 pKa = 3.19QSDD569 pKa = 3.49TDD571 pKa = 3.54NDD573 pKa = 4.47GVGDD577 pKa = 4.14ACDD580 pKa = 3.54VCPDD584 pKa = 3.83SNDD587 pKa = 4.01TIDD590 pKa = 3.61TDD592 pKa = 4.35GDD594 pKa = 4.49GIPDD598 pKa = 3.94GCDD601 pKa = 2.69VCAEE605 pKa = 4.12SDD607 pKa = 3.74DD608 pKa = 4.86TIDD611 pKa = 3.56TDD613 pKa = 4.6GDD615 pKa = 4.64SIPDD619 pKa = 3.72GCDD622 pKa = 2.88NCPTISNPDD631 pKa = 3.32QADD634 pKa = 3.41TDD636 pKa = 3.88GDD638 pKa = 3.94RR639 pKa = 11.84QGDD642 pKa = 4.01VCDD645 pKa = 3.72STPTGDD651 pKa = 3.38VDD653 pKa = 4.81NDD655 pKa = 3.95GVDD658 pKa = 3.54NAMDD662 pKa = 3.49QCNNTTPGVNVDD674 pKa = 3.33ASGCFTLPANNFTIEE689 pKa = 4.63TISEE693 pKa = 4.23TCPDD697 pKa = 4.03KK698 pKa = 11.67DD699 pKa = 3.44NGQILISAQEE709 pKa = 3.93NYY711 pKa = 9.36TYY713 pKa = 11.2NVTLNGVNATLQNNNLTPGNYY734 pKa = 7.7TVCINVAGEE743 pKa = 4.42SYY745 pKa = 9.16EE746 pKa = 3.73QCYY749 pKa = 10.59DD750 pKa = 3.58VIIAEE755 pKa = 4.45GTTISGKK762 pKa = 9.85VAVTSGKK769 pKa = 10.61ASIQIEE775 pKa = 4.26QGTAPFTVFVNDD787 pKa = 4.68KK788 pKa = 10.47IALEE792 pKa = 4.06SAAPIFTVDD801 pKa = 3.34VKK803 pKa = 11.01HH804 pKa = 6.6GDD806 pKa = 3.59KK807 pKa = 10.67IQVKK811 pKa = 10.41SSVEE815 pKa = 3.86CEE817 pKa = 4.43GIFSKK822 pKa = 10.8SINLFDD828 pKa = 4.89EE829 pKa = 5.06VIAYY833 pKa = 7.79PNPTKK838 pKa = 11.01GNFEE842 pKa = 3.85IATPSNQNNIKK853 pKa = 10.42VEE855 pKa = 4.02IFNMQSQLISAKK867 pKa = 10.65NYY869 pKa = 7.57TLNNGKK875 pKa = 10.15VYY877 pKa = 11.02LNIKK881 pKa = 8.47NQAPGVYY888 pKa = 8.97FAKK891 pKa = 10.62VYY893 pKa = 10.41LDD895 pKa = 3.6KK896 pKa = 11.04PIMLKK901 pKa = 10.1IIKK904 pKa = 9.27EE905 pKa = 4.06

Molecular weight: 98.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8AUH4|A0A4Y8AUH4_9FLAO L-fucose:H+ symporter permease OS=Gramella jeungdoensis OX=708091 GN=fucP PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.24RR22 pKa = 11.84MASATGRR29 pKa = 11.84KK30 pKa = 8.64VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.02GRR40 pKa = 11.84NRR42 pKa = 11.84VSVSSDD48 pKa = 3.1PRR50 pKa = 11.84PKK52 pKa = 10.39KK53 pKa = 10.52

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3093

0

3093

1079700

34

2820

349.1

39.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.094 ± 0.039

0.74 ± 0.014

5.171 ± 0.036

6.654 ± 0.04

5.363 ± 0.034

6.221 ± 0.043

1.667 ± 0.019

8.696 ± 0.055

8.506 ± 0.057

9.265 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.026

6.733 ± 0.049

3.243 ± 0.021

3.1 ± 0.024

3.068 ± 0.03

6.444 ± 0.036

5.841 ± 0.046

6.051 ± 0.035

1.053 ± 0.017

4.035 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski