Klebsiella phage IME304

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; unclassified Przondovirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TXT4|A0A4Y5TXT4_9CAUD Tail tubular protein A OS=Klebsiella phage IME304 OX=2584486 PE=4 SV=1
MM1 pKa = 7.38NMQDD5 pKa = 3.87AYY7 pKa = 10.87FGAAAEE13 pKa = 4.02LDD15 pKa = 3.86AVNEE19 pKa = 3.86MLAAIGEE26 pKa = 4.7SPVTTLDD33 pKa = 3.33EE34 pKa = 5.18DD35 pKa = 4.25GSADD39 pKa = 3.27VANARR44 pKa = 11.84RR45 pKa = 11.84ILNRR49 pKa = 11.84INRR52 pKa = 11.84QIQSKK57 pKa = 9.61GWAFNINEE65 pKa = 4.55SATLTPDD72 pKa = 2.99ASTGLIPFRR81 pKa = 11.84PAYY84 pKa = 10.44LSILGGQYY92 pKa = 10.17VNRR95 pKa = 11.84GGWVYY100 pKa = 11.2DD101 pKa = 3.54KK102 pKa = 11.07STGTDD107 pKa = 3.14TFSGPITVTLITLQDD122 pKa = 3.68YY123 pKa = 11.66DD124 pKa = 4.32EE125 pKa = 4.72MPEE128 pKa = 5.08CFRR131 pKa = 11.84QWIVTKK137 pKa = 10.66ASRR140 pKa = 11.84QFNSRR145 pKa = 11.84FFGAEE150 pKa = 3.73DD151 pKa = 3.76VEE153 pKa = 4.46NSLAQEE159 pKa = 3.88EE160 pKa = 4.75MEE162 pKa = 5.66ARR164 pKa = 11.84MACNEE169 pKa = 3.84YY170 pKa = 10.91EE171 pKa = 4.14MDD173 pKa = 3.84FGQYY177 pKa = 11.01NMLDD181 pKa = 3.06GDD183 pKa = 4.58AYY185 pKa = 10.18VQGLIGRR192 pKa = 4.48

Molecular weight: 21.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TVI5|A0A4Y5TVI5_9CAUD Terminase small subunit OS=Klebsiella phage IME304 OX=2584486 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84LHH4 pKa = 6.7FNKK7 pKa = 10.46SNGIFSVRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.94DD18 pKa = 3.43RR19 pKa = 11.84STVVASEE26 pKa = 4.04RR27 pKa = 11.84HH28 pKa = 4.88GKK30 pKa = 8.94IPRR33 pKa = 11.84IGDD36 pKa = 3.64TFEE39 pKa = 3.92LAPRR43 pKa = 11.84VHH45 pKa = 7.31ILVTRR50 pKa = 11.84GLYY53 pKa = 9.93EE54 pKa = 4.12LAQTKK59 pKa = 9.86SRR61 pKa = 11.84PFVPVVVTKK70 pKa = 10.32WPLLRR75 pKa = 11.84LFWEE79 pKa = 5.0RR80 pKa = 11.84IKK82 pKa = 11.06EE83 pKa = 4.25VVNDD87 pKa = 3.83

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11511

38

1321

267.7

29.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.957 ± 0.517

1.042 ± 0.193

6.238 ± 0.183

6.976 ± 0.433

3.414 ± 0.163

8.262 ± 0.415

1.807 ± 0.253

4.839 ± 0.256

6.811 ± 0.443

8.21 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.186

4.239 ± 0.25

3.744 ± 0.198

4.135 ± 0.357

5.404 ± 0.244

6.185 ± 0.438

5.525 ± 0.345

6.95 ± 0.417

1.451 ± 0.213

3.206 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski