Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; Aeropyrum; Aeropyrum pernix

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1700 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9YEJ5|Q9YEJ5_AERPE 2-oxoacid oxidoreductase (ferredoxin) OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=APE_0583 PE=4 SV=1
MM1 pKa = 7.53GVDD4 pKa = 4.05LKK6 pKa = 10.67MLAVALALSAILVGGGLYY24 pKa = 10.32AASALAHH31 pKa = 6.45GGGQGVGGMMGGSQPIASPGMGGMDD56 pKa = 3.43GHH58 pKa = 7.09DD59 pKa = 3.72EE60 pKa = 4.51PYY62 pKa = 10.55EE63 pKa = 4.3DD64 pKa = 6.02DD65 pKa = 4.17YY66 pKa = 12.15GEE68 pKa = 4.54CPMMEE73 pKa = 4.27YY74 pKa = 9.61MHH76 pKa = 6.78MGGMMGMPGPWQGGGWMQGGMGYY99 pKa = 10.61VFMDD103 pKa = 3.41QDD105 pKa = 3.79SMEE108 pKa = 4.14EE109 pKa = 3.58ALAEE113 pKa = 4.21VEE115 pKa = 4.34GVVVNALDD123 pKa = 3.85RR124 pKa = 11.84YY125 pKa = 9.25NAIVVDD131 pKa = 4.0TGDD134 pKa = 3.26GTVRR138 pKa = 11.84AKK140 pKa = 10.35ILPAYY145 pKa = 9.56IDD147 pKa = 3.58VEE149 pKa = 4.1NGYY152 pKa = 8.46MVSGAWIFEE161 pKa = 4.48NIEE164 pKa = 3.89KK165 pKa = 10.48AVEE168 pKa = 3.89DD169 pKa = 3.63RR170 pKa = 11.84GEE172 pKa = 4.17EE173 pKa = 3.94VSVRR177 pKa = 11.84LTIFRR182 pKa = 11.84GGAWTAAVAIEE193 pKa = 3.94IDD195 pKa = 3.87GVGTFVFPALYY206 pKa = 10.12DD207 pKa = 3.62ALNN210 pKa = 3.49

Molecular weight: 21.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q05DW8|Q05DW8_AERPE Uncharacterized protein OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=APE_2473a PE=4 SV=1
MM1 pKa = 7.58ARR3 pKa = 11.84NKK5 pKa = 10.28PLGRR9 pKa = 11.84KK10 pKa = 8.82LRR12 pKa = 11.84LARR15 pKa = 11.84ALKK18 pKa = 10.18SNRR21 pKa = 11.84AIPVWVVIRR30 pKa = 11.84TSRR33 pKa = 11.84RR34 pKa = 11.84IRR36 pKa = 11.84FNLLRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.95WRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 11.16LKK50 pKa = 9.99VV51 pKa = 3.04

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1700

0

1700

492043

30

1950

289.4

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.767 ± 0.068

0.672 ± 0.022

4.389 ± 0.042

7.65 ± 0.064

2.934 ± 0.039

8.867 ± 0.064

1.501 ± 0.022

5.691 ± 0.055

4.143 ± 0.06

11.061 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.211 ± 0.021

1.939 ± 0.029

5.104 ± 0.043

1.686 ± 0.028

7.768 ± 0.084

6.264 ± 0.061

4.205 ± 0.048

9.269 ± 0.058

1.319 ± 0.027

3.561 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski