Campoletis sonorensis ichnovirus (strain Texas A&M) (CsIV)
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q77ZH4|Q77ZH4_CSIVT Cysteine-rich protein OS=Campoletis sonorensis ichnovirus (strain Texas A&M) OX=654920 GN=WHv1.0 PE=4 SV=1
MM1 pKa = 7.19 KK2 pKa = 10.53 VLWFLLGAAVMVQARR17 pKa = 11.84 PDD19 pKa = 3.52 MVSGEE24 pKa = 4.33 KK25 pKa = 10.51 SGDD28 pKa = 3.21 WASEE32 pKa = 4.16 TEE34 pKa = 4.77 FIVTEE39 pKa = 4.61 DD40 pKa = 4.07 GIKK43 pKa = 10.53 PNPNYY48 pKa = 10.23 VKK50 pKa = 10.31 QDD52 pKa = 3.63 GVSSPPVSGWTEE64 pKa = 3.67 DD65 pKa = 3.4 RR66 pKa = 11.84 EE67 pKa = 4.32 FAVTSSTAGPEE78 pKa = 4.21 PKK80 pKa = 10.27 SSVINIDD87 pKa = 3.07 VTKK90 pKa = 10.35 PITAEE95 pKa = 3.64 LTINFHH101 pKa = 5.55 QVQDD105 pKa = 4.13 PAPKK109 pKa = 9.93 PEE111 pKa = 4.62 PSCIDD116 pKa = 2.66 NWKK119 pKa = 8.82 YY120 pKa = 10.7 CRR122 pKa = 11.84 GINKK126 pKa = 8.17 PCCGQQLMEE135 pKa = 6.03 DD136 pKa = 3.8 GTLGPKK142 pKa = 10.18 HH143 pKa = 5.78 FVCFEE148 pKa = 4.62 LGQGICTPLSQIKK161 pKa = 10.24 NADD164 pKa = 3.8 LFAKK168 pKa = 10.56 LADD171 pKa = 3.7 QLNDD175 pKa = 3.14 TNYY178 pKa = 11.22 AEE180 pKa = 6.1 LEE182 pKa = 4.09 SQYY185 pKa = 9.59 WKK187 pKa = 10.55 SVSSPEE193 pKa = 4.18 SSSTFF198 pKa = 3.22
Molecular weight: 21.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.596
IPC2_protein 4.635
IPC_protein 4.533
Toseland 4.38
ProMoST 4.647
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.355
Rodwell 4.38
Grimsley 4.291
Solomon 4.482
Lehninger 4.431
Nozaki 4.596
DTASelect 4.749
Thurlkill 4.38
EMBOSS 4.38
Sillero 4.647
Patrickios 3.745
IPC_peptide 4.482
IPC2_peptide 4.635
IPC2.peptide.svr19 4.599
Protein with the highest isoelectric point:
>tr|Q8JV09|Q8JV09_CSIVT Innexin-like protein 2 OS=Campoletis sonorensis ichnovirus (strain Texas A&M) OX=654920 PE=3 SV=1
MM1 pKa = 7.35 LKK3 pKa = 9.87 IFRR6 pKa = 11.84 TLRR9 pKa = 11.84 GLLKK13 pKa = 10.47 VHH15 pKa = 6.97 VISIDD20 pKa = 3.33 NNFFILHH27 pKa = 5.04 YY28 pKa = 9.75 KK29 pKa = 7.43 ITVVILLALAMLVTSQQFFKK49 pKa = 11.49 NPMEE53 pKa = 5.0 CNFSDD58 pKa = 4.13 LPLGSSHH65 pKa = 6.23 YY66 pKa = 9.8 CYY68 pKa = 10.98 VHH70 pKa = 6.29 ATFLEE75 pKa = 4.34 QQQITHH81 pKa = 6.66 HH82 pKa = 6.26 VPPQRR87 pKa = 11.84 LPGGNISGEE96 pKa = 4.16 TGEE99 pKa = 4.81 KK100 pKa = 10.14 EE101 pKa = 3.77 FRR103 pKa = 11.84 FYY105 pKa = 11.36 NYY107 pKa = 10.47 YY108 pKa = 9.19 EE109 pKa = 4.06 WVYY112 pKa = 9.98 LTLAVQAILFYY123 pKa = 10.52 VPHH126 pKa = 6.68 YY127 pKa = 9.73 IWKK130 pKa = 8.48 AWEE133 pKa = 4.35 GGKK136 pKa = 9.11 MKK138 pKa = 10.05 MLAVEE143 pKa = 4.73 FASPVLSEE151 pKa = 5.19 DD152 pKa = 3.77 FIEE155 pKa = 4.4 NKK157 pKa = 9.32 MIPVVEE163 pKa = 4.23 YY164 pKa = 10.56 FCTTLHH170 pKa = 5.79 SHH172 pKa = 6.08 NAYY175 pKa = 9.39 AYY177 pKa = 9.65 KK178 pKa = 10.79 YY179 pKa = 6.86 FTCEE183 pKa = 3.49 FLNLVNVVGQILFLKK198 pKa = 10.19 IFLGEE203 pKa = 3.92 EE204 pKa = 4.01 FASFGIDD211 pKa = 3.71 VITFDD216 pKa = 3.92 HH217 pKa = 6.23 RR218 pKa = 11.84 QEE220 pKa = 4.14 KK221 pKa = 10.29 SMKK224 pKa = 10.31 NPIDD228 pKa = 3.39 RR229 pKa = 11.84 LFPIVTRR236 pKa = 11.84 CSYY239 pKa = 10.81 HH240 pKa = 6.96 KK241 pKa = 10.02 YY242 pKa = 9.42 GPSGKK247 pKa = 9.51 VEE249 pKa = 3.84 NWEE252 pKa = 4.19 GLCLLPEE259 pKa = 4.12 NSLNGKK265 pKa = 9.48 IYY267 pKa = 10.37 IFMWFWFHH275 pKa = 6.48 MLTAISSVVVIYY287 pKa = 10.34 RR288 pKa = 11.84 IVTLCSPSVRR298 pKa = 11.84 LYY300 pKa = 10.62 RR301 pKa = 11.84 FKK303 pKa = 10.68 PLSGLIRR310 pKa = 11.84 SEE312 pKa = 4.83 DD313 pKa = 3.33 IAIVFPKK320 pKa = 10.35 LNVGIGFCCVGCNGISTRR338 pKa = 11.84 LHH340 pKa = 5.44 TKK342 pKa = 10.18 RR343 pKa = 11.84 SFLALQSTLARR354 pKa = 11.84 PLQMLKK360 pKa = 10.74 VIADD364 pKa = 3.52 VKK366 pKa = 10.8 LALL369 pKa = 4.36
Molecular weight: 42.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.951
IPC2_protein 8.024
IPC_protein 7.863
Toseland 7.6
ProMoST 8.404
Dawson 8.58
Bjellqvist 8.873
Wikipedia 8.536
Rodwell 8.609
Grimsley 7.614
Solomon 8.668
Lehninger 8.668
Nozaki 9.019
DTASelect 8.624
Thurlkill 8.682
EMBOSS 8.77
Sillero 8.975
Patrickios 3.808
IPC_peptide 8.653
IPC2_peptide 7.863
IPC2.peptide.svr19 7.986
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1457
198
369
291.4
33.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.873 ± 0.271
3.02 ± 0.479
4.53 ± 0.761
7.001 ± 1.119
5.971 ± 0.761
5.559 ± 0.423
2.471 ± 0.51
6.452 ± 0.561
6.04 ± 0.595
9.54 ± 1.003
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.402 ± 0.381
4.736 ± 0.283
5.903 ± 0.717
3.089 ± 0.356
3.569 ± 0.546
7.344 ± 0.59
5.353 ± 0.213
6.726 ± 0.413
1.647 ± 0.163
3.775 ± 0.618
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here