Mycobacterium virus Larva
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1FMW2|G1FMW2_9CAUD Uncharacterized protein OS=Mycobacterium virus Larva OX=1056831 GN=60 PE=4 SV=1
MM1 pKa = 6.92 TVGMTAGAEE10 pKa = 3.72 RR11 pKa = 11.84 SAAATHH17 pKa = 6.22 CVVADD22 pKa = 4.38 HH23 pKa = 6.66 YY24 pKa = 10.87 CTGDD28 pKa = 3.51 DD29 pKa = 4.12 CFHH32 pKa = 6.97 CEE34 pKa = 3.64 RR35 pKa = 11.84 RR36 pKa = 11.84 ISQVEE41 pKa = 3.88 YY42 pKa = 10.91 EE43 pKa = 4.14 RR44 pKa = 11.84 DD45 pKa = 3.54 CYY47 pKa = 11.03 RR48 pKa = 11.84 DD49 pKa = 3.71 DD50 pKa = 6.11 DD51 pKa = 4.3 YY52 pKa = 11.89 PDD54 pKa = 4.4 YY55 pKa = 11.65 YY56 pKa = 11.27 DD57 pKa = 3.5 GTT59 pKa = 4.14
Molecular weight: 6.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.221
IPC2_protein 4.342
IPC_protein 4.24
Toseland 4.05
ProMoST 4.406
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.19
Rodwell 4.075
Grimsley 3.961
Solomon 4.228
Lehninger 4.19
Nozaki 4.355
DTASelect 4.609
Thurlkill 4.101
EMBOSS 4.19
Sillero 4.368
Patrickios 0.261
IPC_peptide 4.228
IPC2_peptide 4.355
IPC2.peptide.svr19 4.263
Protein with the highest isoelectric point:
>tr|G1FMV3|G1FMV3_9CAUD Uncharacterized protein OS=Mycobacterium virus Larva OX=1056831 GN=51 PE=4 SV=1
MM1 pKa = 7.29 AAKK4 pKa = 10.35 DD5 pKa = 3.71 AVCWTTCPHH14 pKa = 6.1 CGKK17 pKa = 10.32 RR18 pKa = 11.84 SYY20 pKa = 10.49 TSRR23 pKa = 11.84 KK24 pKa = 7.47 DD25 pKa = 3.07 AKK27 pKa = 10.03 RR28 pKa = 11.84 AVSRR32 pKa = 11.84 FRR34 pKa = 11.84 GDD36 pKa = 2.77 RR37 pKa = 11.84 GGHH40 pKa = 4.46 MSPYY44 pKa = 9.32 RR45 pKa = 11.84 CHH47 pKa = 7.13 DD48 pKa = 3.99 DD49 pKa = 3.14 EE50 pKa = 5.03 SRR52 pKa = 11.84 WHH54 pKa = 6.21 VGHH57 pKa = 7.36 LHH59 pKa = 5.18 TATRR63 pKa = 11.84 RR64 pKa = 11.84 GIHH67 pKa = 5.05 YY68 pKa = 7.22 EE69 pKa = 3.61 QRR71 pKa = 11.84 ARR73 pKa = 11.84 QQ74 pKa = 3.33
Molecular weight: 8.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.428
IPC_protein 9.999
Toseland 10.204
ProMoST 9.955
Dawson 10.394
Bjellqvist 10.145
Wikipedia 10.599
Rodwell 10.584
Grimsley 10.467
Solomon 10.467
Lehninger 10.438
Nozaki 10.306
DTASelect 10.116
Thurlkill 10.262
EMBOSS 10.613
Sillero 10.335
Patrickios 10.321
IPC_peptide 10.467
IPC2_peptide 9.487
IPC2.peptide.svr19 8.34
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
97
0
97
19655
31
1370
202.6
21.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.831 ± 0.413
1.018 ± 0.136
6.757 ± 0.202
5.469 ± 0.3
2.646 ± 0.132
8.695 ± 0.45
2.264 ± 0.174
4.04 ± 0.153
3.378 ± 0.196
7.835 ± 0.182
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.121
2.869 ± 0.166
5.525 ± 0.233
3.465 ± 0.159
7.627 ± 0.391
5.027 ± 0.231
6.477 ± 0.262
7.779 ± 0.254
1.974 ± 0.12
2.178 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here