Bahia Grande virus
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R266|A0A0D3R266_9RHAB GDP polyribonucleotidyltransferase OS=Bahia Grande virus OX=932699 PE=4 SV=1
MM1 pKa = 7.52 AAAILPVSRR10 pKa = 11.84 NMPVRR15 pKa = 11.84 EE16 pKa = 3.85 RR17 pKa = 11.84 TVAGSVTAPPVQYY30 pKa = 10.42 PSTWFQAHH38 pKa = 7.22 AGQKK42 pKa = 10.03 VSITIYY48 pKa = 10.13 QNTNARR54 pKa = 11.84 QAFSRR59 pKa = 11.84 ITQLRR64 pKa = 11.84 NNGQWDD70 pKa = 4.05 DD71 pKa = 3.77 KK72 pKa = 11.49 LIATFMKK79 pKa = 10.7 GVLDD83 pKa = 5.18 EE84 pKa = 4.2 NAEE87 pKa = 4.23 WFQSPPLIEE96 pKa = 4.27 DD97 pKa = 4.1 WIVNEE102 pKa = 3.94 AVIGRR107 pKa = 11.84 VDD109 pKa = 4.31 DD110 pKa = 4.31 VVAPTALAQWEE121 pKa = 4.42 EE122 pKa = 4.26 VEE124 pKa = 5.18 RR125 pKa = 11.84 PQNMDD130 pKa = 3.53 PVPNEE135 pKa = 3.7 EE136 pKa = 4.61 GEE138 pKa = 4.22 LGTRR142 pKa = 11.84 RR143 pKa = 11.84 SFFLALITIYY153 pKa = 10.51 RR154 pKa = 11.84 QVLTRR159 pKa = 11.84 TINVDD164 pKa = 3.05 YY165 pKa = 9.93 GQEE168 pKa = 3.69 VSRR171 pKa = 11.84 RR172 pKa = 11.84 IIDD175 pKa = 3.59 NFKK178 pKa = 9.93 EE179 pKa = 4.17 QPLGMSQDD187 pKa = 4.91 DD188 pKa = 3.5 INEE191 pKa = 3.95 IQGYY195 pKa = 6.75 EE196 pKa = 4.03 SKK198 pKa = 10.88 EE199 pKa = 3.91 RR200 pKa = 11.84 LTTNYY205 pKa = 9.99 VKK207 pKa = 10.35 ILCILDD213 pKa = 3.17 MFFNKK218 pKa = 9.61 FQTHH222 pKa = 6.87 DD223 pKa = 3.18 KK224 pKa = 8.98 STIRR228 pKa = 11.84 IATLPTRR235 pKa = 11.84 YY236 pKa = 9.38 RR237 pKa = 11.84 GCAAFTSYY245 pKa = 11.65 GEE247 pKa = 3.81 LAIRR251 pKa = 11.84 LGIEE255 pKa = 4.31 PIKK258 pKa = 10.67 LPSLILTVAVAKK270 pKa = 10.81 DD271 pKa = 3.39 FDD273 pKa = 4.72 KK274 pKa = 11.34 INVNGEE280 pKa = 3.64 QAEE283 pKa = 4.12 QLDD286 pKa = 4.81 GYY288 pKa = 10.81 FPYY291 pKa = 10.44 QLEE294 pKa = 4.24 LGLVKK299 pKa = 10.45 KK300 pKa = 10.36 SAYY303 pKa = 9.96 SAGNCPSLYY312 pKa = 10.7 LWMHH316 pKa = 6.72 TIGTMLHH323 pKa = 4.69 QQRR326 pKa = 11.84 SYY328 pKa = 10.61 RR329 pKa = 11.84 ANVPKK334 pKa = 10.53 NVPDD338 pKa = 3.35 QMGTINSAIAVAMQFVAGGEE358 pKa = 3.93 FSMQFVGDD366 pKa = 3.84 ARR368 pKa = 11.84 VQEE371 pKa = 4.75 AMRR374 pKa = 11.84 EE375 pKa = 4.18 MQTAEE380 pKa = 4.04 AEE382 pKa = 4.25 LNEE385 pKa = 4.27 LRR387 pKa = 11.84 MAQARR392 pKa = 11.84 EE393 pKa = 3.63 MRR395 pKa = 11.84 AAARR399 pKa = 11.84 GDD401 pKa = 3.39 EE402 pKa = 4.29 DD403 pKa = 4.77 EE404 pKa = 5.13 EE405 pKa = 4.97 GSEE408 pKa = 5.74 DD409 pKa = 5.13 GLDD412 pKa = 4.16 DD413 pKa = 4.55 EE414 pKa = 5.8 NDD416 pKa = 3.99 GEE418 pKa = 5.46 GDD420 pKa = 3.81 DD421 pKa = 4.47 EE422 pKa = 5.01 LPAEE426 pKa = 4.4 IEE428 pKa = 4.18 QNPEE432 pKa = 3.23 YY433 pKa = 10.57 LNRR436 pKa = 11.84 VNRR439 pKa = 11.84 IRR441 pKa = 11.84 EE442 pKa = 4.15 LQEE445 pKa = 4.33 NLQQYY450 pKa = 8.86 NATVQQHH457 pKa = 4.58 TNAVEE462 pKa = 4.03 KK463 pKa = 10.67 AALRR467 pKa = 11.84 ALAYY471 pKa = 9.99 LQEE474 pKa = 4.51 NGGIADD480 pKa = 3.62 KK481 pKa = 10.89 DD482 pKa = 3.71 KK483 pKa = 11.09 RR484 pKa = 11.84 DD485 pKa = 3.23 LGIRR489 pKa = 11.84 FRR491 pKa = 11.84 RR492 pKa = 11.84 FADD495 pKa = 3.51 EE496 pKa = 4.09 AEE498 pKa = 4.09 GRR500 pKa = 11.84 VGKK503 pKa = 10.23 LLASLFPAPRR513 pKa = 4.8
Molecular weight: 57.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.926
IPC2_protein 4.914
IPC_protein 4.851
Toseland 4.711
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.927
Wikipedia 4.635
Rodwell 4.698
Grimsley 4.635
Solomon 4.787
Lehninger 4.736
Nozaki 4.889
DTASelect 5.029
Thurlkill 4.698
EMBOSS 4.66
Sillero 4.965
Patrickios 4.19
IPC_peptide 4.8
IPC2_peptide 4.965
IPC2.peptide.svr19 4.929
Protein with the highest isoelectric point:
>tr|A0A0D3R273|A0A0D3R273_9RHAB Uncharacterized protein OS=Bahia Grande virus OX=932699 PE=4 SV=1
MM1 pKa = 7.55 PQHH4 pKa = 5.57 QTLSQDD10 pKa = 3.63 PPQQPIVFSTVKK22 pKa = 9.99 KK23 pKa = 9.51 VGYY26 pKa = 9.65 IILFSIPLIVIIVFFVLLDD45 pKa = 3.89 RR46 pKa = 11.84 KK47 pKa = 9.28 HH48 pKa = 6.35 LKK50 pKa = 10.22 YY51 pKa = 10.48 IPLKK55 pKa = 10.35 AWNTSIITIKK65 pKa = 10.48 EE66 pKa = 3.92 PYY68 pKa = 9.35
Molecular weight: 7.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.986
IPC2_protein 9.224
IPC_protein 9.165
Toseland 9.838
ProMoST 9.589
Dawson 10.058
Bjellqvist 9.692
Wikipedia 10.218
Rodwell 10.57
Grimsley 10.131
Solomon 10.101
Lehninger 10.072
Nozaki 9.794
DTASelect 9.706
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.955
Patrickios 10.35
IPC_peptide 10.101
IPC2_peptide 8.2
IPC2.peptide.svr19 8.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3920
68
2175
653.3
75.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.49 ± 0.929
1.327 ± 0.318
5.689 ± 0.173
7.781 ± 0.349
4.362 ± 0.355
5.51 ± 0.295
2.143 ± 0.221
7.628 ± 0.356
7.526 ± 0.955
9.464 ± 0.767
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.372 ± 0.104
5.638 ± 0.314
4.056 ± 0.382
3.546 ± 0.675
5.051 ± 0.505
6.964 ± 0.489
5.791 ± 0.168
5.23 ± 0.288
1.48 ± 0.289
3.954 ± 0.414
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here