Halobacterium jilantaiense

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halobacterium

Average proteome isoelectric point is 4.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0PJ56|A0A1I0PJ56_9EURY Uncharacterized protein OS=Halobacterium jilantaiense OX=355548 GN=SAMN04487945_1731 PE=4 SV=1
MM1 pKa = 7.82ADD3 pKa = 4.17DD4 pKa = 3.65NTLTRR9 pKa = 11.84RR10 pKa = 11.84SFLTATGAAAGSVAFVGAGAATPGKK35 pKa = 10.54GRR37 pKa = 11.84GNGRR41 pKa = 11.84GKK43 pKa = 8.87GTEE46 pKa = 3.53RR47 pKa = 11.84RR48 pKa = 11.84EE49 pKa = 4.18YY50 pKa = 10.28IVGVSASEE58 pKa = 4.09DD59 pKa = 3.48DD60 pKa = 3.39PRR62 pKa = 11.84GYY64 pKa = 10.01VRR66 pKa = 11.84KK67 pKa = 9.19HH68 pKa = 5.47LSGKK72 pKa = 8.81EE73 pKa = 3.87EE74 pKa = 4.77PISTNEE80 pKa = 3.82RR81 pKa = 11.84LGTTVVGLPEE91 pKa = 4.01NAAEE95 pKa = 3.97QAHH98 pKa = 5.25QNFLGRR104 pKa = 11.84LEE106 pKa = 4.38AADD109 pKa = 3.46GVKK112 pKa = 10.3YY113 pKa = 10.69VEE115 pKa = 4.55QNVDD119 pKa = 3.23LQAQVAPNDD128 pKa = 3.24PRR130 pKa = 11.84YY131 pKa = 9.58RR132 pKa = 11.84DD133 pKa = 3.4QYY135 pKa = 11.93ADD137 pKa = 3.45QQVNAPEE144 pKa = 4.2AWDD147 pKa = 3.55ATFGDD152 pKa = 3.83SGVTVAVVDD161 pKa = 3.52QGVKK165 pKa = 10.09YY166 pKa = 10.07DD167 pKa = 4.85HH168 pKa = 7.72PDD170 pKa = 3.02LDD172 pKa = 4.3GNMDD176 pKa = 3.71GSVSNSGYY184 pKa = 11.13DD185 pKa = 4.3FVDD188 pKa = 5.06DD189 pKa = 6.14DD190 pKa = 5.45DD191 pKa = 6.29DD192 pKa = 5.04PYY194 pKa = 11.53PDD196 pKa = 4.25ALSDD200 pKa = 3.91EE201 pKa = 4.56YY202 pKa = 11.15HH203 pKa = 5.64GTHH206 pKa = 6.07VGGIAAAEE214 pKa = 4.02VDD216 pKa = 3.5NGEE219 pKa = 4.19GVTGIGNSSLLSGRR233 pKa = 11.84ALSEE237 pKa = 3.94QGSGSTADD245 pKa = 3.35IADD248 pKa = 3.88AVTWAADD255 pKa = 3.29QGADD259 pKa = 4.73VINLSLGGGGYY270 pKa = 8.89TDD272 pKa = 3.24TMKK275 pKa = 10.92NAVSYY280 pKa = 8.31ATNNGALVVAAAGNDD295 pKa = 3.82GQGSVSYY302 pKa = 9.27PAAYY306 pKa = 9.87SEE308 pKa = 4.45CLAVSALDD316 pKa = 3.77PDD318 pKa = 3.94EE319 pKa = 4.65TLASYY324 pKa = 11.31SNYY327 pKa = 10.19GGNIEE332 pKa = 4.42LAAPGTNVLSTWTDD346 pKa = 3.17DD347 pKa = 4.0AYY349 pKa = 11.12DD350 pKa = 4.39SISGTSMATPVVAGVAGLALAQWDD374 pKa = 4.01LTNAEE379 pKa = 4.73LRR381 pKa = 11.84NHH383 pKa = 7.19LKK385 pKa = 8.53NTAVNVGLSSDD396 pKa = 3.43KK397 pKa = 10.86QGSGRR402 pKa = 11.84VDD404 pKa = 3.09AGNAITTEE412 pKa = 4.31PGSGGGGGGGGGGSTSTTISGSLSSYY438 pKa = 10.99ADD440 pKa = 3.85SDD442 pKa = 3.81CSTYY446 pKa = 10.13TFEE449 pKa = 4.91YY450 pKa = 10.23DD451 pKa = 3.33SPSQIVIEE459 pKa = 4.16LSGPSDD465 pKa = 4.11ADD467 pKa = 3.05FDD469 pKa = 5.2LYY471 pKa = 11.65ANTGVGACPTTDD483 pKa = 3.35DD484 pKa = 3.72YY485 pKa = 11.81DD486 pKa = 3.87YY487 pKa = 11.49RR488 pKa = 11.84SYY490 pKa = 8.17TTNSQEE496 pKa = 4.43TITIDD501 pKa = 4.4SPDD504 pKa = 3.67TSTDD508 pKa = 2.99LYY510 pKa = 11.42ALVDD514 pKa = 4.1SYY516 pKa = 11.78SGSGDD521 pKa = 3.28FTVTFTEE528 pKa = 4.51YY529 pKa = 10.23QQ530 pKa = 3.16

Molecular weight: 54.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0QUJ0|A0A1I0QUJ0_9EURY Acyl-CoA thioester hydrolase OS=Halobacterium jilantaiense OX=355548 GN=SAMN04487945_3013 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 5.61PALAALFVGAALASLFMAWVIGAGSSGATPFAPAVGANAIATMRR46 pKa = 11.84AAFVVGLFGFAGAVTQGGSVSEE68 pKa = 4.52AIGGGLVGGASLPVAGVILVLLLGAGLMAVGIQTGYY104 pKa = 10.08PIATAFTVTGAVIGVGLALGGTPVWPKK131 pKa = 9.73YY132 pKa = 9.85RR133 pKa = 11.84QIAAVWALTPFVGGGIAFAIASVLPRR159 pKa = 11.84PGVPEE164 pKa = 4.55RR165 pKa = 11.84YY166 pKa = 9.2SVPVLAGLVAAVLANVEE183 pKa = 4.61FSFLGRR189 pKa = 11.84GASSGSVRR197 pKa = 11.84SLGRR201 pKa = 11.84QALPVGGPTAAAAVTGIAALAVGVVVWWDD230 pKa = 3.12VRR232 pKa = 11.84RR233 pKa = 11.84DD234 pKa = 3.39EE235 pKa = 4.81RR236 pKa = 11.84GGLRR240 pKa = 11.84RR241 pKa = 11.84VLLALGSLVAFSAGGSQVGLAVGPLLPLLDD271 pKa = 4.02EE272 pKa = 4.61VGGLSVFAVLVGGGLGMLVGSWTGAPRR299 pKa = 11.84MIKK302 pKa = 10.14SLAQDD307 pKa = 3.72YY308 pKa = 11.14SSLGPRR314 pKa = 11.84RR315 pKa = 11.84SISALVPSFLIAQLAVLLGVPVSFNEE341 pKa = 4.03IVVSAIIGSGAAVGGRR357 pKa = 11.84AAVDD361 pKa = 3.12ARR363 pKa = 11.84KK364 pKa = 9.76ILVTVGAWAGSFVLSFTLAYY384 pKa = 9.67VAAASLLL391 pKa = 3.87

Molecular weight: 38.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3054

0

3054

884077

39

2195

289.5

31.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.059 ± 0.078

0.666 ± 0.014

9.186 ± 0.057

8.039 ± 0.069

3.277 ± 0.026

8.654 ± 0.046

2.028 ± 0.022

2.965 ± 0.037

1.628 ± 0.027

8.793 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.616 ± 0.018

2.181 ± 0.029

4.604 ± 0.032

2.491 ± 0.032

6.411 ± 0.044

5.405 ± 0.039

6.307 ± 0.045

9.944 ± 0.059

1.125 ± 0.016

2.621 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski