Flavobacterium lacus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328WSS7|A0A328WSS7_9FLAO Uncharacterized protein OS=Flavobacterium lacus OX=1353778 GN=B0I10_1083 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.45KK3 pKa = 8.49ITNLFWLCLIFISSISFAQTARR25 pKa = 11.84VQVIHH30 pKa = 6.62NSPDD34 pKa = 3.23LAASSVDD41 pKa = 3.46VYY43 pKa = 11.66LNDD46 pKa = 3.62VLLLNDD52 pKa = 3.3FHH54 pKa = 7.28FRR56 pKa = 11.84TATPFIDD63 pKa = 3.86APAGVEE69 pKa = 3.83LSIDD73 pKa = 3.5IAPSSSSSSAEE84 pKa = 3.8SIYY87 pKa = 11.33NLTTTLSEE95 pKa = 4.09FEE97 pKa = 4.26TYY99 pKa = 10.24IIIANGIISPTGYY112 pKa = 10.31SPSTPFGLQVLVGGRR127 pKa = 11.84EE128 pKa = 4.2GTAVPDD134 pKa = 3.72TTDD137 pKa = 3.11VIVFHH142 pKa = 7.06GSTDD146 pKa = 3.32APTVDD151 pKa = 3.65VVEE154 pKa = 4.39LAVPAGTIVNDD165 pKa = 3.4ISYY168 pKa = 10.97GSFSEE173 pKa = 4.26YY174 pKa = 11.07LEE176 pKa = 5.33LPTNNYY182 pKa = 8.66VLAVRR187 pKa = 11.84DD188 pKa = 3.74EE189 pKa = 4.45TGTATVATYY198 pKa = 10.01SAPLADD204 pKa = 6.06LFLQTDD210 pKa = 5.22AITVLASGFLDD221 pKa = 4.23PSQNSNGPEE230 pKa = 3.57FGLWVALASGGDD242 pKa = 4.78LIPLPLFHH250 pKa = 7.08NPTARR255 pKa = 11.84LQVIHH260 pKa = 6.66NSPDD264 pKa = 3.25LAASAVDD271 pKa = 3.79VYY273 pKa = 12.02VNGALLLDD281 pKa = 3.65NFAFRR286 pKa = 11.84TATPFIDD293 pKa = 4.01VPAGIALSIDD303 pKa = 3.65VAPSTSTSSAEE314 pKa = 3.81SLYY317 pKa = 11.36NLTATLNEE325 pKa = 4.03FEE327 pKa = 4.7TYY329 pKa = 10.14IIVANGIVSPTGYY342 pKa = 10.38SPNQPFALEE351 pKa = 4.02VLVGGRR357 pKa = 11.84EE358 pKa = 4.14GTAVEE363 pKa = 4.48DD364 pKa = 3.86TTDD367 pKa = 3.35VIVFHH372 pKa = 6.82GSPDD376 pKa = 3.49APTVDD381 pKa = 3.49VVEE384 pKa = 4.46TSVPAGTLVDD394 pKa = 3.63NLDD397 pKa = 3.61YY398 pKa = 11.5GSFSNYY404 pKa = 10.25LEE406 pKa = 5.17LPTNNYY412 pKa = 8.97TIDD415 pKa = 3.43VRR417 pKa = 11.84DD418 pKa = 3.5EE419 pKa = 4.24TGTVTVATYY428 pKa = 9.97SAPLADD434 pKa = 6.22LFLQLDD440 pKa = 5.16AITVVASGFLDD451 pKa = 4.18PSQNSNGPAFGLWVAKK467 pKa = 9.25ATGGALIPLPLVEE480 pKa = 4.71TPTARR485 pKa = 11.84LQAIHH490 pKa = 6.57NSADD494 pKa = 3.2LAAALVDD501 pKa = 3.73VYY503 pKa = 11.9VNDD506 pKa = 3.92EE507 pKa = 4.09LLLNDD512 pKa = 4.06FAFRR516 pKa = 11.84TATPFIDD523 pKa = 3.66VPAGVEE529 pKa = 3.76LSIDD533 pKa = 3.76VAPSTSTSSAEE544 pKa = 4.04SIYY547 pKa = 11.3NLTATLAEE555 pKa = 4.45GEE557 pKa = 4.49TYY559 pKa = 10.54ILVANGIVSPTGYY572 pKa = 10.21SVAPNFAINVFAQGRR587 pKa = 11.84EE588 pKa = 3.99VAANSANTDD597 pKa = 3.19VLVNHH602 pKa = 7.34GSPDD606 pKa = 3.55APTVDD611 pKa = 3.49VVEE614 pKa = 4.29TSIPAGTIVDD624 pKa = 4.07NISFPEE630 pKa = 3.98FQGYY634 pKa = 10.53LDD636 pKa = 4.91LPNLDD641 pKa = 3.35FTLDD645 pKa = 3.5VRR647 pKa = 11.84DD648 pKa = 3.55EE649 pKa = 4.44TGTVTVASYY658 pKa = 9.19QAPLQTLGLEE668 pKa = 4.52GAAITVLASGFLDD681 pKa = 4.21PSANSNGPAFGLWVALAEE699 pKa = 4.63GGALIPLPLVEE710 pKa = 4.71TPTARR715 pKa = 11.84LQAIHH720 pKa = 6.59NSPDD724 pKa = 3.0LAAALVDD731 pKa = 3.73VYY733 pKa = 11.9VNDD736 pKa = 3.92EE737 pKa = 4.09LLLNDD742 pKa = 4.06FAFRR746 pKa = 11.84TATPFIDD753 pKa = 3.66VPAGVEE759 pKa = 3.76LSIDD763 pKa = 3.76VAPSTSTSSAEE774 pKa = 4.04SIYY777 pKa = 11.3NLTATLAEE785 pKa = 4.45GEE787 pKa = 4.49TYY789 pKa = 10.54ILVANGIVSPTGYY802 pKa = 10.27SVGPNFAINVFAQGRR817 pKa = 11.84EE818 pKa = 3.99VAANSANTDD827 pKa = 3.19VLVNHH832 pKa = 7.34GSPDD836 pKa = 3.55APTVDD841 pKa = 3.49VVEE844 pKa = 4.29TSIPAGTIVNNISFPEE860 pKa = 4.0FQGYY864 pKa = 10.53LDD866 pKa = 4.91LPNLDD871 pKa = 3.35FTLDD875 pKa = 3.5VRR877 pKa = 11.84DD878 pKa = 3.55EE879 pKa = 4.44TGTVTVASYY888 pKa = 9.19QAPLQTLGLEE898 pKa = 4.52GAAITVLASGFLDD911 pKa = 4.21PSANSNGPAFGLWVALASGGDD932 pKa = 4.63LIPLPEE938 pKa = 5.26IPLNVDD944 pKa = 3.3SFNLNSISLFPNPAKK959 pKa = 9.79EE960 pKa = 4.34TINLSIPFDD969 pKa = 3.85YY970 pKa = 11.1EE971 pKa = 3.87NFSATIYY978 pKa = 10.4DD979 pKa = 3.68LSGRR983 pKa = 11.84LVKK986 pKa = 9.83TVTTPTDD993 pKa = 3.07IDD995 pKa = 3.35ISEE998 pKa = 4.69LNSGLYY1004 pKa = 9.79ILNTTLNDD1012 pKa = 3.38LSFNQKK1018 pKa = 8.42FVKK1021 pKa = 10.35NN1022 pKa = 3.81

Molecular weight: 107.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328WLQ6|A0A328WLQ6_9FLAO N-dimethylarginine dimethylaminohydrolase OS=Flavobacterium lacus OX=1353778 GN=B0I10_111100 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 5.49AITTDD27 pKa = 3.56KK28 pKa = 11.07PEE30 pKa = 4.39KK31 pKa = 10.56SLLAPKK37 pKa = 10.25NNSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.44KK59 pKa = 9.52QKK61 pKa = 10.78YY62 pKa = 9.58RR63 pKa = 11.84IIDD66 pKa = 3.78FKK68 pKa = 10.28RR69 pKa = 11.84TKK71 pKa = 10.51DD72 pKa = 3.79GIPATVKK79 pKa = 10.64SIEE82 pKa = 4.0YY83 pKa = 10.15DD84 pKa = 3.43PNRR87 pKa = 11.84TAFIALLAYY96 pKa = 10.34ADD98 pKa = 3.82GAKK101 pKa = 9.85TYY103 pKa = 10.97VIAQNGLNVGQTVTSGEE120 pKa = 4.06GSAPEE125 pKa = 4.24IGNALPLSKK134 pKa = 10.33IPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAIIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCMATIGAVSNSDD199 pKa = 2.96HH200 pKa = 6.02QLIVSGKK207 pKa = 8.58AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.29GYY255 pKa = 6.86RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.63VSPSNKK266 pKa = 9.58YY267 pKa = 9.24IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3053

0

3053

1057804

29

3746

346.5

39.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.175 ± 0.047

0.791 ± 0.017

5.147 ± 0.033

6.512 ± 0.059

5.648 ± 0.039

6.28 ± 0.06

1.662 ± 0.022

8.081 ± 0.044

7.544 ± 0.085

9.153 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.144 ± 0.024

6.594 ± 0.05

3.439 ± 0.035

3.578 ± 0.024

3.148 ± 0.032

6.599 ± 0.044

6.173 ± 0.087

6.377 ± 0.038

1.012 ± 0.017

3.945 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski