Streptococcus satellite phage Javan15

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFK2|A0A4D5ZFK2_9VIRU Putative transcription regulator OS=Streptococcus satellite phage Javan15 OX=2558543 GN=JavanS15_0005 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 5.48LNDD5 pKa = 3.77RR6 pKa = 11.84LKK8 pKa = 10.48IEE10 pKa = 4.16EE11 pKa = 4.31MEE13 pKa = 4.18EE14 pKa = 4.52KK15 pKa = 10.56YY16 pKa = 11.09DD17 pKa = 4.01SFKK20 pKa = 11.02PRR22 pKa = 11.84INALVEE28 pKa = 4.77AIDD31 pKa = 5.09DD32 pKa = 4.11FQKK35 pKa = 10.73HH36 pKa = 4.85YY37 pKa = 10.99EE38 pKa = 4.3DD39 pKa = 3.9YY40 pKa = 10.25VKK42 pKa = 10.67LRR44 pKa = 11.84EE45 pKa = 4.27FYY47 pKa = 10.94GSEE50 pKa = 3.33DD51 pKa = 3.14WFRR54 pKa = 11.84LSEE57 pKa = 4.0QTEE60 pKa = 4.21NNLKK64 pKa = 10.71CGVLSEE70 pKa = 4.72DD71 pKa = 3.9QLFDD75 pKa = 4.9FIGEE79 pKa = 4.14HH80 pKa = 6.24NEE82 pKa = 4.15LVGQFLDD89 pKa = 4.22MSSQMYY95 pKa = 9.86RR96 pKa = 11.84HH97 pKa = 6.4LL98 pKa = 4.33

Molecular weight: 11.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZER9|A0A4D5ZER9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan15 OX=2558543 GN=JavanS15_0020 PE=4 SV=1
MM1 pKa = 7.72LKK3 pKa = 9.85NQNNMIKK10 pKa = 10.25KK11 pKa = 9.22KK12 pKa = 10.2RR13 pKa = 11.84NPRR16 pKa = 11.84VLFRR20 pKa = 11.84NVAYY24 pKa = 9.65KK25 pKa = 10.68LSEE28 pKa = 4.15IEE30 pKa = 4.57GKK32 pKa = 8.08TLTEE36 pKa = 3.88TASFSWFWEE45 pKa = 4.11FRR47 pKa = 11.84SLQKK51 pKa = 10.68RR52 pKa = 11.84PIQMEE57 pKa = 3.94AA58 pKa = 2.95

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

3791

39

500

145.8

16.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.355 ± 0.317

0.58 ± 0.195

5.619 ± 0.461

8.336 ± 0.469

4.563 ± 0.257

4.221 ± 0.324

1.899 ± 0.245

7.122 ± 0.474

9.47 ± 0.413

9.654 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.25

5.724 ± 0.309

3.086 ± 0.49

4.247 ± 0.295

4.906 ± 0.341

5.592 ± 0.376

6.199 ± 0.519

5.17 ± 0.329

0.976 ± 0.153

5.065 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski