Flavobacterium phage FCOV-F36
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G8L832|A0A7G8L832_9CAUD Uncharacterized protein OS=Flavobacterium phage FCOV-F36 OX=2764976 PE=4 SV=1
MM1 pKa = 8.28 LEE3 pKa = 3.85 IFFTINGIDD12 pKa = 3.38 QKK14 pKa = 10.67 EE15 pKa = 3.91 IAVFYY20 pKa = 10.96 DD21 pKa = 3.81 NEE23 pKa = 4.04 DD24 pKa = 2.72 LKK26 pKa = 11.6 YY27 pKa = 10.41 FVEE30 pKa = 4.98 TYY32 pKa = 10.32 KK33 pKa = 10.93 EE34 pKa = 4.05 QVLEE38 pKa = 4.09 QYY40 pKa = 7.58 PTATNIRR47 pKa = 11.84 LGDD50 pKa = 3.94 LSTSLNNYY58 pKa = 7.79 NAA60 pKa = 4.02
Molecular weight: 7.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.888
IPC2_protein 4.202
IPC_protein 4.024
Toseland 3.859
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.884
Rodwell 3.859
Grimsley 3.77
Solomon 3.961
Lehninger 3.91
Nozaki 4.113
DTASelect 4.24
Thurlkill 3.897
EMBOSS 3.897
Sillero 4.139
Patrickios 1.926
IPC_peptide 3.961
IPC2_peptide 4.113
IPC2.peptide.svr19 4.027
Protein with the highest isoelectric point:
>tr|A0A7G8L7X0|A0A7G8L7X0_9CAUD Uncharacterized protein OS=Flavobacterium phage FCOV-F36 OX=2764976 PE=4 SV=1
MM1 pKa = 7.56 RR2 pKa = 11.84 AWNANLRR9 pKa = 11.84 LKK11 pKa = 10.72 ANRR14 pKa = 11.84 VVYY17 pKa = 10.17 RR18 pKa = 11.84 AYY20 pKa = 8.46 THH22 pKa = 6.43 NMVKK26 pKa = 8.85 PTGTTDD32 pKa = 3.4 YY33 pKa = 10.89 VSVSFVLPVKK43 pKa = 10.66 SDD45 pKa = 3.41 FLANGGLLYY54 pKa = 10.87 VNPNTGITITNRR66 pKa = 11.84 YY67 pKa = 7.56 IAAGLLHH74 pKa = 6.79 KK75 pKa = 10.68 GVV77 pKa = 3.71
Molecular weight: 8.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 10.131
IPC_protein 10.613
Toseland 10.335
ProMoST 10.116
Dawson 10.555
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.862
Grimsley 10.657
Solomon 10.599
Lehninger 10.555
Nozaki 10.292
DTASelect 10.277
Thurlkill 10.394
EMBOSS 10.745
Sillero 10.467
Patrickios 10.599
IPC_peptide 10.599
IPC2_peptide 9.136
IPC2.peptide.svr19 8.305
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
0
76
14872
34
729
195.7
22.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.184 ± 0.451
0.955 ± 0.121
5.769 ± 0.199
7.0 ± 0.368
4.794 ± 0.179
5.507 ± 0.295
1.352 ± 0.129
7.955 ± 0.262
9.42 ± 0.424
8.849 ± 0.187
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.125 ± 0.145
7.383 ± 0.259
2.858 ± 0.139
3.618 ± 0.186
3.51 ± 0.201
6.61 ± 0.254
5.843 ± 0.273
5.836 ± 0.215
1.069 ± 0.1
4.364 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here