Paenibacillus sp. FSL H7-0737

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089IMS5|A0A089IMS5_9BACL Transcriptional regulator OS=Paenibacillus sp. FSL H7-0737 OX=1536775 GN=H70737_17420 PE=4 SV=1
MM1 pKa = 7.68VNIQLSPSTSTQFEE15 pKa = 3.97PTIAVNWLNPSVMVAVAVDD34 pKa = 4.39LRR36 pKa = 11.84TGPPNIGLYY45 pKa = 10.44KK46 pKa = 10.66SIDD49 pKa = 3.53AGATWTTTLLPLPTGFAGIEE69 pKa = 4.13APHH72 pKa = 7.27IDD74 pKa = 4.29YY75 pKa = 10.69IFPTTFVVLAHH86 pKa = 6.81AFDD89 pKa = 4.36SDD91 pKa = 3.92GLSGSIVSYY100 pKa = 10.09TSVDD104 pKa = 3.36NASSFGPPVIVNQGFGQFVNDD125 pKa = 3.89DD126 pKa = 3.69QVVVVTDD133 pKa = 3.87KK134 pKa = 11.41AGSSPFFGNVYY145 pKa = 9.58TGYY148 pKa = 7.65THH150 pKa = 8.11DD151 pKa = 4.95YY152 pKa = 7.71NTQFIPGNTIFFNRR166 pKa = 11.84SLDD169 pKa = 3.42SGNTYY174 pKa = 10.19FSPALLSSVEE184 pKa = 4.1EE185 pKa = 4.24FEE187 pKa = 4.51EE188 pKa = 4.85FPGIAITLNGIVVVGWINQPPGNTDD213 pKa = 3.21LKK215 pKa = 10.89IRR217 pKa = 11.84TSPDD221 pKa = 2.66GGVTFTPEE229 pKa = 3.5TMVASVVVPPDD240 pKa = 3.84PLPGYY245 pKa = 7.23TFRR248 pKa = 11.84CLTYY252 pKa = 9.85PSLAADD258 pKa = 4.03ISNAPTTQGNVYY270 pKa = 10.49AVWQDD275 pKa = 3.26FRR277 pKa = 11.84EE278 pKa = 5.26GYY280 pKa = 10.46SDD282 pKa = 3.69IFLATSTNFGVDD294 pKa = 2.62WGTPISITGSPAGSQNFFPAITVSPADD321 pKa = 3.26GAIFVTYY328 pKa = 7.63YY329 pKa = 9.45TNRR332 pKa = 11.84VNPLNLDD339 pKa = 3.44VYY341 pKa = 10.88LATSRR346 pKa = 11.84DD347 pKa = 3.24GGMTFTNTRR356 pKa = 11.84ITTTSFDD363 pKa = 3.39VTGIPLIGDD372 pKa = 3.89YY373 pKa = 10.22IGNAIVPQTGRR384 pKa = 11.84LVSVWTDD391 pKa = 2.74TRR393 pKa = 11.84TGTEE397 pKa = 4.28NIWFGDD403 pKa = 3.68NQQ405 pKa = 3.46

Molecular weight: 43.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089IV89|A0A089IV89_9BACL Uncharacterized protein OS=Paenibacillus sp. FSL H7-0737 OX=1536775 GN=H70737_28590 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5383

0

5383

1768007

26

2501

328.4

36.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.639 ± 0.034

0.755 ± 0.011

5.097 ± 0.027

6.761 ± 0.038

4.258 ± 0.028

7.306 ± 0.026

2.015 ± 0.015

7.224 ± 0.033

5.588 ± 0.031

10.249 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.017

4.173 ± 0.023

3.847 ± 0.017

3.691 ± 0.021

4.512 ± 0.022

6.652 ± 0.027

5.574 ± 0.031

6.988 ± 0.027

1.235 ± 0.012

3.561 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski