Camelus dromedarius papillomavirus 2
Average proteome isoelectric point is 7.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2YGI1|F2YGI1_9PAPI Major capsid protein L1 OS=Camelus dromedarius papillomavirus 2 OX=996651 GN=L1 PE=3 SV=1
MM1 pKa = 7.83 ASRR4 pKa = 11.84 KK5 pKa = 8.71 RR6 pKa = 11.84 VRR8 pKa = 11.84 RR9 pKa = 11.84 ANPYY13 pKa = 9.77 EE14 pKa = 3.99 LYY16 pKa = 8.96 RR17 pKa = 11.84 TCKK20 pKa = 9.96 AANTCPRR27 pKa = 11.84 DD28 pKa = 3.74 VIPLVEE34 pKa = 4.69 GNTLADD40 pKa = 4.74 QILKK44 pKa = 8.1 WGSWAVYY51 pKa = 10.18 LGGLGIGTGTQSISGRR67 pKa = 11.84 PITTGSVKK75 pKa = 10.89 SNIEE79 pKa = 3.81 KK80 pKa = 10.63 FLVNLFGKK88 pKa = 10.7 GGTQTRR94 pKa = 11.84 APTGGIPLEE103 pKa = 4.12 TLGAGTSLGTPDD115 pKa = 3.55 VAVTATDD122 pKa = 3.33 APTVVVPEE130 pKa = 4.4 APSGGEE136 pKa = 3.73 PGEE139 pKa = 4.07 GLGVVTEE146 pKa = 4.38 NTQEE150 pKa = 4.0 TAFTYY155 pKa = 10.93 VDD157 pKa = 3.25 VDD159 pKa = 3.66 DD160 pKa = 4.5 TGEE163 pKa = 3.91 IMVVNLRR170 pKa = 11.84 PDD172 pKa = 3.69 GDD174 pKa = 4.61 DD175 pKa = 3.47 PTSHH179 pKa = 7.1 LSSSTTFHH187 pKa = 6.5 NPTYY191 pKa = 9.97 QEE193 pKa = 3.56 PSGRR197 pKa = 11.84 LEE199 pKa = 4.42 TVGEE203 pKa = 4.18 TSNTEE208 pKa = 4.16 NIFLAGEE215 pKa = 4.22 NLGSTGGEE223 pKa = 3.96 EE224 pKa = 4.05 IEE226 pKa = 4.86 LSLFSKK232 pKa = 10.42 PRR234 pKa = 11.84 TSTPRR239 pKa = 11.84 QSPRR243 pKa = 11.84 APVRR247 pKa = 11.84 GRR249 pKa = 11.84 ANWFSRR255 pKa = 11.84 RR256 pKa = 11.84 YY257 pKa = 7.69 YY258 pKa = 9.53 TQLNLNNPAVITQPGEE274 pKa = 3.96 VIGTQFVNPVFEE286 pKa = 4.64 GSSFEE291 pKa = 4.4 APPAYY296 pKa = 9.84 EE297 pKa = 3.9 SWNTEE302 pKa = 3.82 LPEE305 pKa = 4.67 LRR307 pKa = 11.84 DD308 pKa = 3.63 ATHH311 pKa = 5.48 YY312 pKa = 7.7 TASRR316 pKa = 11.84 LLQGPSGRR324 pKa = 11.84 VGVDD328 pKa = 3.14 RR329 pKa = 11.84 VATTRR334 pKa = 11.84 TIGTRR339 pKa = 11.84 SGIRR343 pKa = 11.84 IGPEE347 pKa = 3.3 VHH349 pKa = 6.1 VRR351 pKa = 11.84 QSLSSISDD359 pKa = 3.14 AVEE362 pKa = 3.6 FATFVSGDD370 pKa = 3.54 VEE372 pKa = 4.22 TAPTVEE378 pKa = 4.07 TAFVEE383 pKa = 4.66 EE384 pKa = 4.27 TAPFEE389 pKa = 6.17 DD390 pKa = 3.64 IDD392 pKa = 4.49 LDD394 pKa = 4.15 SVYY397 pKa = 11.03 SDD399 pKa = 4.39 EE400 pKa = 5.95 DD401 pKa = 4.19 LLDD404 pKa = 3.61 QYY406 pKa = 11.23 PATSHH411 pKa = 5.42 GRR413 pKa = 11.84 LVFGEE418 pKa = 4.18 RR419 pKa = 11.84 FNRR422 pKa = 11.84 DD423 pKa = 2.88 IVPIPIDD430 pKa = 3.71 PPFTALVPVDD440 pKa = 3.24 VTTYY444 pKa = 7.86 VTPGANWPDD453 pKa = 3.64 FTPGSKK459 pKa = 10.49 GGMTPGIIIDD469 pKa = 3.86 LSEE472 pKa = 4.22 EE473 pKa = 4.21 LFKK476 pKa = 10.68 HH477 pKa = 5.86 YY478 pKa = 10.29 YY479 pKa = 9.25 LHH481 pKa = 7.39 EE482 pKa = 4.43 SLLRR486 pKa = 11.84 RR487 pKa = 11.84 KK488 pKa = 9.46 RR489 pKa = 11.84 RR490 pKa = 11.84 RR491 pKa = 11.84 RR492 pKa = 11.84 KK493 pKa = 9.54 RR494 pKa = 11.84 LYY496 pKa = 10.37 AA497 pKa = 4.23
Molecular weight: 53.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.034
IPC2_protein 5.054
IPC_protein 4.991
Toseland 4.902
ProMoST 5.105
Dawson 4.952
Bjellqvist 5.092
Wikipedia 4.813
Rodwell 4.876
Grimsley 4.825
Solomon 4.952
Lehninger 4.914
Nozaki 5.067
DTASelect 5.207
Thurlkill 4.889
EMBOSS 4.838
Sillero 5.143
Patrickios 4.177
IPC_peptide 4.965
IPC2_peptide 5.143
IPC2.peptide.svr19 5.114
Protein with the highest isoelectric point:
>tr|F2YGH8|F2YGH8_9PAPI Replication protein E1 OS=Camelus dromedarius papillomavirus 2 OX=996651 GN=E1 PE=3 SV=1
MM1 pKa = 7.49 AAAEE5 pKa = 4.3 DD6 pKa = 4.22 TPPRR10 pKa = 11.84 PGPSSRR16 pKa = 11.84 CPPSIAPTVKK26 pKa = 9.97 HH27 pKa = 6.13 RR28 pKa = 11.84 CPACKK33 pKa = 9.68 GVHH36 pKa = 6.07 GPITYY41 pKa = 8.44 PQLPCLSLIKK51 pKa = 9.83 WNPPTWVPSEE61 pKa = 4.26 SAVLGPLHH69 pKa = 6.1 VLRR72 pKa = 11.84 PSRR75 pKa = 11.84 YY76 pKa = 8.46 YY77 pKa = 10.61 GAQGSRR83 pKa = 11.84 NAYY86 pKa = 9.8 LPPPTCRR93 pKa = 11.84 ARR95 pKa = 11.84 SRR97 pKa = 11.84 KK98 pKa = 8.56 VWHH101 pKa = 6.13 EE102 pKa = 3.25 HH103 pKa = 5.23 RR104 pKa = 11.84 KK105 pKa = 7.29 WMKK108 pKa = 9.69 RR109 pKa = 11.84 RR110 pKa = 11.84 HH111 pKa = 5.5 LRR113 pKa = 11.84 TPRR116 pKa = 11.84 RR117 pKa = 11.84 YY118 pKa = 8.97 PP119 pKa = 3.2
Molecular weight: 13.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 10.014
IPC_protein 11.038
Toseland 11.023
ProMoST 11.096
Dawson 11.096
Bjellqvist 10.935
Wikipedia 11.418
Rodwell 11.14
Grimsley 11.155
Solomon 11.359
Lehninger 11.301
Nozaki 11.038
DTASelect 10.935
Thurlkill 11.038
EMBOSS 11.462
Sillero 11.052
Patrickios 10.862
IPC_peptide 11.359
IPC2_peptide 10.321
IPC2.peptide.svr19 8.471
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2432
119
642
347.4
38.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.401 ± 0.887
2.426 ± 0.588
5.222 ± 0.32
5.387 ± 0.472
3.454 ± 0.408
7.196 ± 0.702
2.22 ± 0.32
3.331 ± 0.382
5.428 ± 0.828
7.936 ± 0.326
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.521 ± 0.248
3.824 ± 0.492
7.319 ± 1.334
4.235 ± 0.539
6.414 ± 0.717
7.689 ± 0.716
6.867 ± 1.013
6.826 ± 0.406
1.521 ± 0.218
3.783 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here