Nora virus
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2WFA0|D2WFA0_NORAV Capsid protein OS=Nora virus OX=363716 PE=1 SV=1
MM1 pKa = 7.49 ALKK4 pKa = 10.69 EE5 pKa = 4.29 EE6 pKa = 4.59 IFDD9 pKa = 4.12 QNTTLFAVLDD19 pKa = 4.21 EE20 pKa = 4.76 NEE22 pKa = 3.99 VTEE25 pKa = 4.41 IKK27 pKa = 10.44 SIQASVTAVKK37 pKa = 8.71 TQLDD41 pKa = 3.88 QQKK44 pKa = 10.19 LQLDD48 pKa = 4.31 GLAKK52 pKa = 10.68 VVDD55 pKa = 3.81 NNQARR60 pKa = 11.84 NEE62 pKa = 4.02 EE63 pKa = 4.09 QFVNINTTLVEE74 pKa = 4.06 MSLEE78 pKa = 4.05 VDD80 pKa = 3.28 KK81 pKa = 10.86 LTTTTSQQAKK91 pKa = 9.66 QINTFATALNEE102 pKa = 3.88 LDD104 pKa = 3.22 QTTRR108 pKa = 11.84 DD109 pKa = 3.71 SLDD112 pKa = 3.41 TLNTTTEE119 pKa = 4.2 SLTKK123 pKa = 10.19 QVLFNTDD130 pKa = 3.53 EE131 pKa = 4.11 ITVLKK136 pKa = 10.7 VDD138 pKa = 3.81 VATVTQKK145 pKa = 10.26 QQDD148 pKa = 3.96 VEE150 pKa = 4.42 HH151 pKa = 7.19 SLEE154 pKa = 4.15 TMKK157 pKa = 11.23 DD158 pKa = 3.57 EE159 pKa = 4.45 IGEE162 pKa = 4.05 LHH164 pKa = 6.48 VSVNANVNSIEE175 pKa = 3.97 ALRR178 pKa = 11.84 TRR180 pKa = 11.84 IAALEE185 pKa = 3.98 IRR187 pKa = 11.84 DD188 pKa = 3.72 VGPWVLKK195 pKa = 10.26 DD196 pKa = 4.12 RR197 pKa = 11.84 IYY199 pKa = 11.25 KK200 pKa = 9.59 FVINKK205 pKa = 9.37 PNGTTRR211 pKa = 11.84 YY212 pKa = 3.95 TTIYY216 pKa = 10.02 FFADD220 pKa = 3.57 VYY222 pKa = 11.19 YY223 pKa = 9.58 STGVRR228 pKa = 11.84 AAPTNSGTATSILTITSLTTSYY250 pKa = 11.52 SLANVPVLKK259 pKa = 10.42 GVPYY263 pKa = 10.4 RR264 pKa = 11.84 VNGYY268 pKa = 8.54 FANGNNIEE276 pKa = 5.34 DD277 pKa = 3.84 ITGSTSVIYY286 pKa = 10.68 DD287 pKa = 3.4 SMM289 pKa = 5.66
Molecular weight: 32.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.679
IPC2_protein 4.622
IPC_protein 4.546
Toseland 4.393
ProMoST 4.66
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.368
Rodwell 4.38
Grimsley 4.291
Solomon 4.495
Lehninger 4.444
Nozaki 4.609
DTASelect 4.774
Thurlkill 4.393
EMBOSS 4.393
Sillero 4.66
Patrickios 4.062
IPC_peptide 4.495
IPC2_peptide 4.647
IPC2.peptide.svr19 4.62
Protein with the highest isoelectric point:
>tr|D2WF98|D2WF98_NORAV Replication polyprotein OS=Nora virus OX=363716 PE=4 SV=1
MM1 pKa = 7.82 INNQTNKK8 pKa = 10.17 KK9 pKa = 9.21 GPQLEE14 pKa = 4.1 RR15 pKa = 11.84 VHH17 pKa = 6.82 FGSTQVVGKK26 pKa = 7.26 STKK29 pKa = 9.43 RR30 pKa = 11.84 RR31 pKa = 11.84 QRR33 pKa = 11.84 GTKK36 pKa = 9.87 LDD38 pKa = 2.94 IEE40 pKa = 4.57 YY41 pKa = 9.64 TVRR44 pKa = 11.84 RR45 pKa = 11.84 NDD47 pKa = 3.13 APKK50 pKa = 9.57 EE51 pKa = 3.95 QKK53 pKa = 10.53 FLISEE58 pKa = 4.1 IFDD61 pKa = 3.51 EE62 pKa = 5.62 KK63 pKa = 10.92 LDD65 pKa = 3.78 KK66 pKa = 10.51 QIKK69 pKa = 8.41 YY70 pKa = 9.44 EE71 pKa = 4.05 KK72 pKa = 9.47 KK73 pKa = 9.82 QNHH76 pKa = 5.43 TFIKK80 pKa = 10.17 PKK82 pKa = 10.19 LNLVIKK88 pKa = 9.65 EE89 pKa = 4.14 EE90 pKa = 3.78 QHH92 pKa = 4.67 ITKK95 pKa = 10.19 KK96 pKa = 9.93 VLRR99 pKa = 11.84 GKK101 pKa = 9.79 EE102 pKa = 3.64 RR103 pKa = 11.84 AATHH107 pKa = 6.45 AFMKK111 pKa = 10.59 EE112 pKa = 3.77 MVEE115 pKa = 4.24 SNKK118 pKa = 9.58 IQPSWNVEE126 pKa = 3.87 YY127 pKa = 10.38 EE128 pKa = 4.09 KK129 pKa = 11.01 EE130 pKa = 3.82 IDD132 pKa = 3.75 EE133 pKa = 4.38 VDD135 pKa = 3.57 LFFMKK140 pKa = 10.47 KK141 pKa = 7.05 KK142 pKa = 8.73 TKK144 pKa = 9.54 PFSGFSIKK152 pKa = 8.72 EE153 pKa = 3.95 LRR155 pKa = 11.84 DD156 pKa = 3.57 SLIVQSDD163 pKa = 4.6 DD164 pKa = 3.83 KK165 pKa = 12.0 NMAQPTVMSSIDD177 pKa = 3.79 EE178 pKa = 4.08 IVTPRR183 pKa = 11.84 EE184 pKa = 4.17 EE185 pKa = 4.07 ISVSAISEE193 pKa = 4.05 QLASLMEE200 pKa = 4.22 RR201 pKa = 11.84 VDD203 pKa = 4.13 KK204 pKa = 11.07 LEE206 pKa = 4.17 KK207 pKa = 10.09 MNAALEE213 pKa = 4.28 EE214 pKa = 4.32 EE215 pKa = 4.23 NKK217 pKa = 9.9 QLKK220 pKa = 9.77 KK221 pKa = 10.5 EE222 pKa = 3.94 RR223 pKa = 11.84 EE224 pKa = 4.06 ATIKK228 pKa = 10.49 SVKK231 pKa = 10.29 KK232 pKa = 8.67 EE233 pKa = 3.78 AKK235 pKa = 9.75 KK236 pKa = 10.34 IKK238 pKa = 9.05 QEE240 pKa = 3.72 KK241 pKa = 7.73 PQIVKK246 pKa = 8.68 KK247 pKa = 7.73 TQHH250 pKa = 6.4 KK251 pKa = 10.37 SLGVNLKK258 pKa = 8.15 ITKK261 pKa = 9.03 TKK263 pKa = 10.75 VVGQEE268 pKa = 3.49 QCLEE272 pKa = 4.1 IEE274 pKa = 4.36 NTQHH278 pKa = 6.69 KK279 pKa = 10.39 KK280 pKa = 10.27 FVEE283 pKa = 4.4 KK284 pKa = 10.32 PSMPLKK290 pKa = 10.16 VSKK293 pKa = 10.76 KK294 pKa = 6.04 MTEE297 pKa = 3.86 HH298 pKa = 5.76 QLKK301 pKa = 8.55 KK302 pKa = 9.28 TIRR305 pKa = 11.84 TWYY308 pKa = 9.89 EE309 pKa = 3.28 FDD311 pKa = 3.51 PSKK314 pKa = 10.74 LVQHH318 pKa = 5.85 QKK320 pKa = 10.43 EE321 pKa = 4.36 VLNSVVTNTTFADD334 pKa = 3.72 KK335 pKa = 10.81 VRR337 pKa = 11.84 EE338 pKa = 3.97 TGIPKK343 pKa = 10.0 QKK345 pKa = 9.63 IRR347 pKa = 11.84 YY348 pKa = 6.1 VAKK351 pKa = 10.12 PPAEE355 pKa = 4.37 EE356 pKa = 3.73 KK357 pKa = 10.65 RR358 pKa = 11.84 SIHH361 pKa = 6.45 FYY363 pKa = 10.09 GYY365 pKa = 9.89 KK366 pKa = 10.05 PKK368 pKa = 10.89 GIPNKK373 pKa = 9.81 VWWNWVTTGTAMDD386 pKa = 4.52 AYY388 pKa = 9.51 EE389 pKa = 4.06 KK390 pKa = 10.71 ADD392 pKa = 3.62 RR393 pKa = 11.84 YY394 pKa = 10.58 LYY396 pKa = 10.37 HH397 pKa = 5.51 QFKK400 pKa = 10.5 RR401 pKa = 11.84 EE402 pKa = 3.58 MMIYY406 pKa = 9.28 RR407 pKa = 11.84 NKK409 pKa = 8.55 WVKK412 pKa = 9.94 FSKK415 pKa = 10.41 EE416 pKa = 3.69 FNPYY420 pKa = 9.42 LSKK423 pKa = 11.02 PKK425 pKa = 9.88 MVWEE429 pKa = 4.26 EE430 pKa = 3.98 NTWEE434 pKa = 4.07 YY435 pKa = 10.99 EE436 pKa = 4.19 YY437 pKa = 10.27 KK438 pKa = 10.38 TDD440 pKa = 3.64 VPYY443 pKa = 11.45 NFILKK448 pKa = 8.63 WRR450 pKa = 11.84 QLVQTYY456 pKa = 9.99 KK457 pKa = 10.82 PNTPIQADD465 pKa = 3.79 WYY467 pKa = 10.68 KK468 pKa = 10.56 ISQKK472 pKa = 7.53 QQCC475 pKa = 3.79
Molecular weight: 56.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.877
IPC2_protein 9.019
IPC_protein 8.858
Toseland 9.926
ProMoST 9.472
Dawson 10.072
Bjellqvist 9.663
Wikipedia 10.189
Rodwell 10.774
Grimsley 10.116
Solomon 10.087
Lehninger 10.058
Nozaki 9.897
DTASelect 9.663
Thurlkill 9.94
EMBOSS 10.306
Sillero 9.984
Patrickios 10.394
IPC_peptide 10.087
IPC2_peptide 8.009
IPC2.peptide.svr19 7.943
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3799
289
2104
949.8
107.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.87 ± 0.585
0.921 ± 0.296
5.238 ± 0.494
6.844 ± 0.623
3.79 ± 0.123
5.265 ± 0.712
1.764 ± 0.158
7.318 ± 0.357
7.37 ± 1.88
8.66 ± 0.902
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.053 ± 0.209
5.791 ± 0.353
4.291 ± 0.876
4.396 ± 0.493
4.528 ± 0.233
6.238 ± 0.265
7.37 ± 1.046
7.476 ± 0.636
1.474 ± 0.257
3.343 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here