Wuchan romanomermis nematode virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEY7|A0A1L3KEY7_9VIRU Uncharacterized protein OS=Wuchan romanomermis nematode virus 3 OX=1923687 PE=4 SV=1
MM1 pKa = 7.27WWDD4 pKa = 4.55FYY6 pKa = 9.39TWKK9 pKa = 10.35PYY11 pKa = 9.94IYY13 pKa = 10.37SVAGFNFTARR23 pKa = 11.84APQGLIPGEE32 pKa = 4.44LYY34 pKa = 9.31WLTVRR39 pKa = 11.84VVGPFLDD46 pKa = 3.41QFPFQPALSFSGFEE60 pKa = 3.94LVATLFSAIDD70 pKa = 3.41QLYY73 pKa = 8.85SAGVNVLTRR82 pKa = 11.84WVTKK86 pKa = 9.83IVVRR90 pKa = 11.84HH91 pKa = 4.34VTVDD95 pKa = 3.2PSYY98 pKa = 11.54SVDD101 pKa = 3.36PEE103 pKa = 3.69ILVRR107 pKa = 11.84FGQRR111 pKa = 11.84HH112 pKa = 4.17VDD114 pKa = 3.15IFAAAVRR121 pKa = 11.84DD122 pKa = 3.56YY123 pKa = 11.55ARR125 pKa = 11.84IYY127 pKa = 10.33ISMVFDD133 pKa = 3.68SLSGYY138 pKa = 8.87WPDD141 pKa = 4.1VPNQVDD147 pKa = 3.6ANSEE151 pKa = 4.26RR152 pKa = 11.84IGIPRR157 pKa = 11.84PMGDD161 pKa = 3.32GADD164 pKa = 3.64SLEE167 pKa = 4.39TSWEE171 pKa = 4.3VVSEE175 pKa = 4.33GG176 pKa = 4.04

Molecular weight: 19.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEX8|A0A1L3KEX8_9VIRU Uncharacterized protein OS=Wuchan romanomermis nematode virus 3 OX=1923687 PE=4 SV=1
MM1 pKa = 7.11LQAPVRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84QNNNGGLIGQLVRR26 pKa = 11.84QLASLEE32 pKa = 4.15LARR35 pKa = 11.84APAVQQPQPARR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84GAGGAGGLITAGPSATVATRR74 pKa = 11.84GGPSFANSPQGGLRR88 pKa = 11.84LVNEE92 pKa = 4.79EE93 pKa = 4.03LWKK96 pKa = 11.09SADD99 pKa = 2.92ITTADD104 pKa = 4.22FLQKK108 pKa = 10.04LTFTPGKK115 pKa = 10.04SGLTILDD122 pKa = 4.53RR123 pKa = 11.84IAATFDD129 pKa = 3.29RR130 pKa = 11.84YY131 pKa = 10.07VVHH134 pKa = 6.67SVSLSWRR141 pKa = 11.84TSVGTQKK148 pKa = 10.87DD149 pKa = 3.36GAYY152 pKa = 10.46VIGIEE157 pKa = 3.89WDD159 pKa = 3.42ASKK162 pKa = 9.03TAPNYY167 pKa = 11.0ADD169 pKa = 3.48AQALVPRR176 pKa = 11.84IRR178 pKa = 11.84SAIWKK183 pKa = 8.31EE184 pKa = 3.82EE185 pKa = 3.74QMVLPSEE192 pKa = 4.33RR193 pKa = 11.84LMSRR197 pKa = 11.84RR198 pKa = 11.84FYY200 pKa = 10.86STGFDD205 pKa = 3.86DD206 pKa = 5.97AATKK210 pKa = 11.01NPDD213 pKa = 3.21CTAFAAILAVKK224 pKa = 10.02GVASAGNVGDD234 pKa = 3.04VWIRR238 pKa = 11.84YY239 pKa = 8.43DD240 pKa = 3.41ISLMGPTSANSKK252 pKa = 8.17TAVLEE257 pKa = 4.11NN258 pKa = 3.76

Molecular weight: 28.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1218

176

448

304.5

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.031 ± 0.965

1.149 ± 0.374

5.747 ± 0.398

4.516 ± 0.623

4.023 ± 0.873

7.882 ± 0.816

1.478 ± 0.308

4.598 ± 0.33

3.777 ± 0.554

9.113 ± 0.568

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.364

2.709 ± 0.455

5.255 ± 0.302

4.433 ± 0.248

7.964 ± 0.832

7.882 ± 0.414

4.351 ± 0.567

7.964 ± 0.917

2.545 ± 0.681

3.448 ± 0.493
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski