Marssonina coronariae
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9355 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A218Z6W4|A0A218Z6W4_9HELO Septin-type G domain-containing protein OS=Marssonina coronariae OX=503106 GN=B2J93_6081 PE=3 SV=1
MM1 pKa = 6.72 QTTLTLAALASAAWAFPHH19 pKa = 6.1 VARR22 pKa = 11.84 DD23 pKa = 3.75 APAGCSTSYY32 pKa = 10.89 EE33 pKa = 3.92 GDD35 pKa = 3.57 FQITILNGSSIITKK49 pKa = 9.83 RR50 pKa = 11.84 DD51 pKa = 3.14 LGEE54 pKa = 4.27 RR55 pKa = 11.84 AASTCGQEE63 pKa = 4.78 GYY65 pKa = 8.48 LTATLKK71 pKa = 10.93 DD72 pKa = 3.51 GNLIDD77 pKa = 3.6 NKK79 pKa = 10.44 GRR81 pKa = 11.84 IGSIVANRR89 pKa = 11.84 QFQFDD94 pKa = 4.28 GPPAQSGAVYY104 pKa = 9.4 TNGWSVCSNGSLATSAGSTLFYY126 pKa = 10.37 SCKK129 pKa = 10.27 SGEE132 pKa = 4.04 FANLYY137 pKa = 10.04 DD138 pKa = 3.88 EE139 pKa = 5.95 SVAEE143 pKa = 4.14 YY144 pKa = 10.01 CLPVYY149 pKa = 10.34 IDD151 pKa = 4.7 VIPCSSAAATGAVPQQADD169 pKa = 4.03 GQPTATTAVAPVTQIADD186 pKa = 3.74 GQPQGKK192 pKa = 6.88 PTAPAVTQIGDD203 pKa = 3.68 GQLQGMPAVTQIADD217 pKa = 3.73 GQLQIPTGAPTAITQIADD235 pKa = 3.73 GQVQAPATAGAPAATQITDD254 pKa = 3.54 GQVQVPTTAAAPATQIGDD272 pKa = 3.66 GQVQVPTVAPTAGATQISDD291 pKa = 3.52 GQVQVPTAVSGPAVSQIPDD310 pKa = 3.26 GQLQSNVSTSATPRR324 pKa = 11.84 PIAVSAAVANSPLFVSALGSAALGFAAVILLL355 pKa = 3.96
Molecular weight: 35.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.817
IPC2_protein 4.062
IPC_protein 4.05
Toseland 3.821
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.999
Rodwell 3.872
Grimsley 3.732
Solomon 4.037
Lehninger 3.999
Nozaki 4.164
DTASelect 4.444
Thurlkill 3.884
EMBOSS 4.012
Sillero 4.177
Patrickios 1.1
IPC_peptide 4.037
IPC2_peptide 4.151
IPC2.peptide.svr19 4.035
Protein with the highest isoelectric point:
>tr|A0A218YX69|A0A218YX69_9HELO Uncharacterized protein OS=Marssonina coronariae OX=503106 GN=B2J93_9443 PE=4 SV=1
MM1 pKa = 7.0 PQPRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 LWTLEE12 pKa = 3.86 SGSTSKK18 pKa = 11.21 VNVRR22 pKa = 11.84 ASVKK26 pKa = 9.67 VNLRR30 pKa = 11.84 ASIKK34 pKa = 9.52 VSIRR38 pKa = 11.84 ASIKK42 pKa = 8.8 VSIRR46 pKa = 11.84 ASIKK50 pKa = 9.6 VNLTARR56 pKa = 11.84 IKK58 pKa = 10.41 VNHH61 pKa = 6.26 PRR63 pKa = 11.84 FF64 pKa = 3.68
Molecular weight: 7.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.379
IPC2_protein 10.818
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.31
Grimsley 12.603
Solomon 13.042
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.047
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.061
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9355
0
9355
4461638
39
6376
476.9
52.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.962 ± 0.023
1.137 ± 0.009
5.474 ± 0.018
6.344 ± 0.028
3.538 ± 0.016
7.196 ± 0.028
2.302 ± 0.011
4.757 ± 0.017
5.002 ± 0.026
8.702 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.122 ± 0.01
3.522 ± 0.014
6.304 ± 0.028
3.871 ± 0.017
6.392 ± 0.022
8.622 ± 0.036
5.842 ± 0.016
6.011 ± 0.017
1.337 ± 0.008
2.562 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here