Ancylostoma caninum (Dog hookworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Strongylida; Ancylostomatoidea; Ancylostomatidae; Ancylostomatinae; Ancylostoma

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29973 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368FN95|A0A368FN95_ANCCA Uncharacterized protein OS=Ancylostoma caninum OX=29170 GN=ANCCAN_22556 PE=4 SV=1
MM1 pKa = 7.74IKK3 pKa = 9.29TFCDD7 pKa = 3.25VFTLQMRR14 pKa = 11.84GQFDD18 pKa = 3.41EE19 pKa = 5.7SEE21 pKa = 4.31YY22 pKa = 11.5QMYY25 pKa = 9.71PPSDD29 pKa = 3.58NRR31 pKa = 11.84NLAIISDD38 pKa = 4.36DD39 pKa = 3.71SLSPCQEE46 pKa = 4.42AIEE49 pKa = 4.66SNQVPQIQILRR60 pKa = 11.84RR61 pKa = 11.84DD62 pKa = 3.4SDD64 pKa = 3.77EE65 pKa = 5.62DD66 pKa = 3.82PDD68 pKa = 4.52AGEE71 pKa = 4.98DD72 pKa = 3.66SMEE75 pKa = 4.79LVSCCC80 pKa = 4.79

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368EXK9|A0A368EXK9_ANCCA Uncharacterized protein OS=Ancylostoma caninum OX=29170 GN=ANCCAN_29790 PE=4 SV=1
MM1 pKa = 7.62LLLPRR6 pKa = 11.84QSLQRR11 pKa = 11.84NLKK14 pKa = 7.79QTLRR18 pKa = 11.84QKK20 pKa = 10.83RR21 pKa = 11.84SLQLQRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 10.03KK30 pKa = 10.15RR31 pKa = 11.84RR32 pKa = 11.84SQRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.44

Molecular weight: 4.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29973

0

29973

7766988

26

5882

259.1

29.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.242 ± 0.015

2.26 ± 0.013

5.326 ± 0.012

6.513 ± 0.02

4.237 ± 0.012

5.768 ± 0.02

2.399 ± 0.008

5.251 ± 0.013

5.636 ± 0.017

8.956 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.008

4.096 ± 0.011

5.247 ± 0.023

3.862 ± 0.013

6.164 ± 0.013

7.808 ± 0.018

5.59 ± 0.016

6.728 ± 0.014

1.174 ± 0.006

3.079 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski