Escherichia phage vB_EcoS_HASG4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus; unclassified Tequintavirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 161 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482N451|A0A482N451_9CAUD Peptidase_M15_4 domain-containing protein OS=Escherichia phage vB_EcoS_HASG4 OX=2508175 GN=HASG4_00041 PE=4 SV=1
MM1 pKa = 7.45GFGYY5 pKa = 10.46DD6 pKa = 3.6FSEE9 pKa = 3.99LRR11 pKa = 11.84EE12 pKa = 4.09VVEE15 pKa = 4.25QIPFISQVLGEE26 pKa = 4.37MCGSSIKK33 pKa = 9.79TPSPATCEE41 pKa = 4.01EE42 pKa = 4.19QPQEE46 pKa = 4.25VLDD49 pKa = 4.07SLSTT53 pKa = 3.55

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482N5Z0|A0A482N5Z0_9CAUD Holin OS=Escherichia phage vB_EcoS_HASG4 OX=2508175 GN=HASG4_00042 PE=3 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.77QKK5 pKa = 10.17NAKK8 pKa = 7.37TQVATTVKK16 pKa = 9.78TFPQTEE22 pKa = 4.31ANRR25 pKa = 11.84KK26 pKa = 8.9ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.6PTDD39 pKa = 3.49AQAAQALNRR48 pKa = 11.84PAPIRR53 pKa = 11.84QKK55 pKa = 10.69PKK57 pKa = 10.23AKK59 pKa = 10.11NATRR63 pKa = 11.84SVARR67 pKa = 11.84LVTYY71 pKa = 9.3VQGYY75 pKa = 5.66GHH77 pKa = 7.13KK78 pKa = 9.77SVPVTLSFNAGAEE91 pKa = 4.17LFSRR95 pKa = 11.84NGMAMKK101 pKa = 10.22DD102 pKa = 3.46YY103 pKa = 10.91EE104 pKa = 4.19KK105 pKa = 10.97AVNQKK110 pKa = 9.97RR111 pKa = 11.84KK112 pKa = 8.13PTADD116 pKa = 3.13VLRR119 pKa = 11.84DD120 pKa = 3.4TRR122 pKa = 11.84GQFGSVKK129 pKa = 9.73PNIFGVEE136 pKa = 3.85YY137 pKa = 11.02SKK139 pKa = 11.81DD140 pKa = 3.37NVRR143 pKa = 11.84ALCYY147 pKa = 10.2GVGIKK152 pKa = 8.48FTGDD156 pKa = 3.39SPRR159 pKa = 11.84KK160 pKa = 7.76SAKK163 pKa = 9.0PARR166 pKa = 11.84KK167 pKa = 9.43RR168 pKa = 11.84KK169 pKa = 9.48VKK171 pKa = 10.62

Molecular weight: 19.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

161

0

161

31399

35

1358

195.0

21.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.612 ± 0.335

1.08 ± 0.099

5.972 ± 0.169

6.905 ± 0.253

4.118 ± 0.136

6.338 ± 0.214

1.844 ± 0.122

6.596 ± 0.171

7.233 ± 0.235

8.551 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.685 ± 0.121

5.392 ± 0.205

3.506 ± 0.159

3.876 ± 0.199

4.446 ± 0.132

6.478 ± 0.256

5.866 ± 0.176

6.382 ± 0.167

1.178 ± 0.092

3.943 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski