Flavobacterium tiangeerense
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1482 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A562L6D4|A0A562L6D4_9FLAO Site-specific DNA-methyltransferase (adenine-specific) OS=Flavobacterium tiangeerense OX=459471 GN=IQ05_00123 PE=3 SV=1
MM1 pKa = 6.29 NTNYY5 pKa = 10.62 KK6 pKa = 10.38 LEE8 pKa = 4.16 SEE10 pKa = 4.48 FLGKK14 pKa = 10.28 VEE16 pKa = 5.19 DD17 pKa = 6.18 DD18 pKa = 4.2 KK19 pKa = 12.12 DD20 pKa = 3.5 EE21 pKa = 4.49 TNNKK25 pKa = 9.07 EE26 pKa = 4.14 LLSEE30 pKa = 4.09 EE31 pKa = 4.06 QLNKK35 pKa = 10.05 IIEE38 pKa = 4.47 KK39 pKa = 10.03 YY40 pKa = 10.35 PNVPLDD46 pKa = 3.61 YY47 pKa = 10.18 IGYY50 pKa = 9.14 LRR52 pKa = 11.84 EE53 pKa = 4.05 IGSGNFRR60 pKa = 11.84 EE61 pKa = 4.64 CQFKK65 pKa = 10.56 VQSFLFNLEE74 pKa = 4.01 DD75 pKa = 3.93 LGLDD79 pKa = 3.02 EE80 pKa = 6.31 HH81 pKa = 8.66 YY82 pKa = 10.56 DD83 pKa = 3.12 IKK85 pKa = 11.36 SNIWFFGDD93 pKa = 3.89 NYY95 pKa = 10.94 CGDD98 pKa = 3.73 FSGFDD103 pKa = 3.51 LDD105 pKa = 5.64 LYY107 pKa = 11.1 DD108 pKa = 4.05 GLVVEE113 pKa = 6.27 FWHH116 pKa = 5.87 EE117 pKa = 4.12 TGEE120 pKa = 4.68 LFYY123 pKa = 11.04 TNQTFQNYY131 pKa = 7.57 IRR133 pKa = 11.84 QQIGIDD139 pKa = 3.48 EE140 pKa = 4.68 NGNDD144 pKa = 3.58 TLEE147 pKa = 4.05
Molecular weight: 17.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.975
IPC2_protein 4.062
IPC_protein 4.012
Toseland 3.834
ProMoST 4.113
Dawson 3.973
Bjellqvist 4.164
Wikipedia 3.872
Rodwell 3.846
Grimsley 3.745
Solomon 3.973
Lehninger 3.923
Nozaki 4.088
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.884
Sillero 4.126
Patrickios 2.715
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 4.034
Protein with the highest isoelectric point:
>tr|A0A562L666|A0A562L666_9FLAO Uncharacterized protein OS=Flavobacterium tiangeerense OX=459471 GN=IQ05_00078 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.91 HH17 pKa = 4.39 GFMDD21 pKa = 4.5 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.41 KK42 pKa = 10.14 LTVSSEE48 pKa = 3.92 PRR50 pKa = 11.84 HH51 pKa = 5.77 KK52 pKa = 10.61 KK53 pKa = 9.84
Molecular weight: 6.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1482
0
1482
497200
39
2691
335.5
37.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.542 ± 0.072
0.747 ± 0.02
5.208 ± 0.04
6.368 ± 0.068
5.397 ± 0.048
6.094 ± 0.068
1.622 ± 0.03
8.273 ± 0.066
8.145 ± 0.075
9.345 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.184 ± 0.031
6.406 ± 0.07
3.28 ± 0.04
3.629 ± 0.036
3.093 ± 0.044
6.521 ± 0.051
5.955 ± 0.059
6.244 ± 0.049
0.974 ± 0.02
3.971 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here