Vibrio phage VALG_phi6

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650EVJ8|A0A650EVJ8_9VIRU Uncharacterized protein OS=Vibrio phage VALG_phi6 OX=2682965 GN=VALGphi6_00080 PE=4 SV=1
MM1 pKa = 7.54NEE3 pKa = 3.73AQIIYY8 pKa = 10.5YY9 pKa = 10.29DD10 pKa = 4.47LLPDD14 pKa = 3.54YY15 pKa = 9.43TVSVLVKK22 pKa = 10.88GCDD25 pKa = 3.08EE26 pKa = 4.05WDD28 pKa = 3.58LLKK31 pKa = 11.25SMSHH35 pKa = 6.11LEE37 pKa = 4.03SWASSQFTSYY47 pKa = 11.06EE48 pKa = 3.99LVSITNTTVEE58 pKa = 3.75QRR60 pKa = 11.84INLGVFDD67 pKa = 5.59DD68 pKa = 4.3YY69 pKa = 12.03CNN71 pKa = 3.32

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650EVX9|A0A650EVX9_9VIRU Putative assembly protein OS=Vibrio phage VALG_phi6 OX=2682965 GN=VALGphi6_00040 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.46KK3 pKa = 10.66LEE5 pKa = 4.08LVVNNVKK12 pKa = 10.37HH13 pKa = 6.11AVVNKK18 pKa = 8.63KK19 pKa = 7.3TAAGAALMVASVSPAFAEE37 pKa = 3.99VDD39 pKa = 2.94ITGAINSAVSGGQANVSLVVAGLIGMAALGFGVTMVVGFLRR80 pKa = 11.84KK81 pKa = 9.45

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2438

47

461

187.5

21.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.645 ± 0.696

1.928 ± 0.287

6.03 ± 0.836

6.112 ± 0.322

4.717 ± 0.492

6.563 ± 0.846

2.297 ± 0.329

6.358 ± 0.544

6.03 ± 0.64

10.049 ± 1.102

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.46

4.963 ± 0.415

3.61 ± 0.568

3.568 ± 0.342

4.143 ± 0.66

6.481 ± 0.739

5.989 ± 0.715

7.055 ± 0.878

1.354 ± 0.207

3.815 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski