Podoviridae sp. ctg2L5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W8J3|A0A5Q2W8J3_9CAUD Uncharacterized protein OS=Podoviridae sp. ctg2L5 OX=2656712 PE=4 SV=1
MM1 pKa = 7.86SYY3 pKa = 11.09QNYY6 pKa = 9.52NCPCSNCGNQGDD18 pKa = 4.26DD19 pKa = 3.72VEE21 pKa = 4.43LTEE24 pKa = 4.41VTVSDD29 pKa = 3.87TGAKK33 pKa = 9.79FDD35 pKa = 3.87IFLCASCQTNDD46 pKa = 3.45YY47 pKa = 11.36GEE49 pKa = 4.53VNTDD53 pKa = 3.05EE54 pKa = 5.43NEE56 pKa = 3.79AFYY59 pKa = 10.21TII61 pKa = 4.37

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W8Q5|A0A5Q2W8Q5_9CAUD Uncharacterized protein OS=Podoviridae sp. ctg2L5 OX=2656712 PE=4 SV=1
MM1 pKa = 7.48NCPICGEE8 pKa = 4.11VLDD11 pKa = 4.8SKK13 pKa = 10.86LKK15 pKa = 10.28KK16 pKa = 10.15YY17 pKa = 10.63CSSKK21 pKa = 10.25CRR23 pKa = 11.84HH24 pKa = 5.93KK25 pKa = 10.72AYY27 pKa = 10.09KK28 pKa = 9.94LRR30 pKa = 11.84NRR32 pKa = 11.84EE33 pKa = 3.08KK34 pKa = 10.71DD35 pKa = 3.17LRR37 pKa = 11.84KK38 pKa = 9.47RR39 pKa = 11.84RR40 pKa = 11.84QYY42 pKa = 10.71NRR44 pKa = 11.84KK45 pKa = 7.53KK46 pKa = 8.73TKK48 pKa = 9.65EE49 pKa = 3.92GYY51 pKa = 8.38FRR53 pKa = 11.84KK54 pKa = 9.65WYY56 pKa = 10.39AKK58 pKa = 9.25NRR60 pKa = 11.84KK61 pKa = 8.82VKK63 pKa = 10.22IKK65 pKa = 10.32PRR67 pKa = 11.84LCDD70 pKa = 3.27YY71 pKa = 10.97CKK73 pKa = 11.3GEE75 pKa = 4.02FTPDD79 pKa = 2.37WRR81 pKa = 11.84HH82 pKa = 4.61NFQRR86 pKa = 11.84FCSLKK91 pKa = 10.36CRR93 pKa = 11.84LKK95 pKa = 10.34NRR97 pKa = 11.84DD98 pKa = 3.28RR99 pKa = 11.84KK100 pKa = 10.27RR101 pKa = 11.84EE102 pKa = 3.8YY103 pKa = 10.53QNNKK107 pKa = 9.74EE108 pKa = 4.2EE109 pKa = 4.3IKK111 pKa = 10.83KK112 pKa = 9.65RR113 pKa = 11.84WKK115 pKa = 10.31DD116 pKa = 3.17YY117 pKa = 9.91RR118 pKa = 11.84DD119 pKa = 2.98RR120 pKa = 11.84VRR122 pKa = 11.84FGKK125 pKa = 10.06KK126 pKa = 9.98GKK128 pKa = 10.3GLLRR132 pKa = 11.84RR133 pKa = 11.84QALKK137 pKa = 10.42RR138 pKa = 11.84DD139 pKa = 3.54NNQCQLCGSKK149 pKa = 10.78DD150 pKa = 3.6KK151 pKa = 11.07IVVHH155 pKa = 5.98HH156 pKa = 6.84CKK158 pKa = 10.63YY159 pKa = 10.38PADD162 pKa = 3.75SLKK165 pKa = 10.5WLLTLCRR172 pKa = 11.84SCHH175 pKa = 5.89ASIHH179 pKa = 4.9NTT181 pKa = 3.24

Molecular weight: 22.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

14333

31

788

188.6

21.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.684 ± 0.27

0.774 ± 0.13

6.021 ± 0.265

7.256 ± 0.419

4.319 ± 0.214

6.907 ± 0.289

1.954 ± 0.251

5.833 ± 0.25

8.003 ± 0.481

7.563 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.17

4.702 ± 0.255

4.193 ± 0.218

3.614 ± 0.306

5.142 ± 0.322

6.928 ± 0.89

6.161 ± 0.818

5.882 ± 0.244

1.654 ± 0.172

4.054 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski