Deferribacter desulfuricans (strain DSM 14783 / JCM 11476 / NBRC 101012 / SSM1)
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2338 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3PDR8|D3PDR8_DEFDS Uncharacterized protein OS=Deferribacter desulfuricans (strain DSM 14783 / JCM 11476 / NBRC 101012 / SSM1) OX=639282 GN=DEFDS_1274 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 KK3 pKa = 10.0 LIFLIVILSFASLLFAGDD21 pKa = 3.7 FTLGSTDD28 pKa = 3.24 NEE30 pKa = 4.65 TIKK33 pKa = 11.06 LSANVSADD41 pKa = 3.12 YY42 pKa = 11.16 DD43 pKa = 3.93 ADD45 pKa = 4.13 TNGNHH50 pKa = 5.25 YY51 pKa = 10.7 VAGTMSSKK59 pKa = 9.3 GTKK62 pKa = 9.8 KK63 pKa = 10.77 YY64 pKa = 9.19 GTADD68 pKa = 3.67 TEE70 pKa = 4.45 SVIYY74 pKa = 10.62 YY75 pKa = 9.19 DD76 pKa = 3.52 EE77 pKa = 4.51 CTGNDD82 pKa = 3.71 CADD85 pKa = 4.15 DD86 pKa = 3.77 NVTFSGYY93 pKa = 10.62 SSGLSTIQGWTPMEE107 pKa = 4.05
Molecular weight: 11.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 4.088
IPC_protein 4.024
Toseland 3.808
ProMoST 4.139
Dawson 4.024
Bjellqvist 4.253
Wikipedia 3.986
Rodwell 3.859
Grimsley 3.719
Solomon 4.012
Lehninger 3.973
Nozaki 4.151
DTASelect 4.406
Thurlkill 3.884
EMBOSS 3.999
Sillero 4.151
Patrickios 0.896
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.059
Protein with the highest isoelectric point:
>tr|D3PB85|D3PB85_DEFDS GIY-YIG domain-containing protein OS=Deferribacter desulfuricans (strain DSM 14783 / JCM 11476 / NBRC 101012 / SSM1) OX=639282 GN=DEFDS_0358 PE=4 SV=1
MM1 pKa = 7.4 LTMKK5 pKa = 10.33 KK6 pKa = 9.94 PSNIKK11 pKa = 9.93 RR12 pKa = 11.84 KK13 pKa = 9.07 RR14 pKa = 11.84 KK15 pKa = 8.63 HH16 pKa = 4.94 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.99 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 LVLKK32 pKa = 10.32 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.6 GRR39 pKa = 11.84 KK40 pKa = 8.88 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.272
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.778
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.501
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2338
0
2338
763288
35
1576
326.5
37.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.28 ± 0.053
0.974 ± 0.021
5.83 ± 0.042
7.062 ± 0.063
5.434 ± 0.048
5.812 ± 0.052
1.485 ± 0.017
9.793 ± 0.054
9.954 ± 0.065
9.794 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.063 ± 0.022
6.197 ± 0.06
3.002 ± 0.034
2.175 ± 0.026
3.258 ± 0.033
5.656 ± 0.039
4.585 ± 0.036
6.499 ± 0.042
0.605 ± 0.017
4.541 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here