candidate division MSBL1 archaeon SCGC-AAA261C02

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133V0Y2|A0A133V0Y2_9EURY Probable tRNA sulfurtransferase OS=candidate division MSBL1 archaeon SCGC-AAA261C02 OX=1698272 GN=thiI PE=3 SV=1
MM1 pKa = 7.72CGQGAIEE8 pKa = 4.65YY9 pKa = 6.97FTIVAALIVVFAGVTITQMVNPFSEE34 pKa = 4.52SARR37 pKa = 11.84EE38 pKa = 3.88EE39 pKa = 4.03EE40 pKa = 4.46QLAQAQVASDD50 pKa = 4.18TIANAINGVYY60 pKa = 10.29ANSEE64 pKa = 4.04GAVITEE70 pKa = 4.2FVNVSKK76 pKa = 10.62SWSLDD81 pKa = 3.5LNSDD85 pKa = 3.7NLRR88 pKa = 11.84VGVEE92 pKa = 3.69IDD94 pKa = 5.6GEE96 pKa = 4.52MEE98 pKa = 3.87WKK100 pKa = 10.26EE101 pKa = 3.83SSLEE105 pKa = 3.92YY106 pKa = 10.97GFDD109 pKa = 3.26NSIPNISSGNYY120 pKa = 6.79TVIVEE125 pKa = 4.22WPSDD129 pKa = 3.22GTEE132 pKa = 4.29NINLDD137 pKa = 3.58SANDD141 pKa = 3.81KK142 pKa = 10.63IYY144 pKa = 10.38IYY146 pKa = 10.2INPGGGGG153 pKa = 3.24

Molecular weight: 16.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133V2D7|A0A133V2D7_9EURY Translation initiation factor 2 subunit gamma OS=candidate division MSBL1 archaeon SCGC-AAA261C02 OX=1698272 GN=eif2g PE=3 SV=1
MM1 pKa = 7.87PATKK5 pKa = 9.99RR6 pKa = 11.84CSFCDD11 pKa = 3.36EE12 pKa = 4.09RR13 pKa = 11.84VTPGRR18 pKa = 11.84GIMYY22 pKa = 8.44VKK24 pKa = 10.26RR25 pKa = 11.84DD26 pKa = 3.24GRR28 pKa = 11.84IQYY31 pKa = 9.3YY32 pKa = 10.24CSSKK36 pKa = 10.16CRR38 pKa = 11.84RR39 pKa = 11.84NAEE42 pKa = 4.38LGRR45 pKa = 11.84KK46 pKa = 5.22PHH48 pKa = 5.68KK49 pKa = 10.73VKK51 pKa = 8.19WTKK54 pKa = 10.24RR55 pKa = 11.84SRR57 pKa = 11.84EE58 pKa = 4.02ARR60 pKa = 11.84GKK62 pKa = 9.54EE63 pKa = 3.81

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

703

0

703

165970

48

1157

236.1

26.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.031 ± 0.105

0.938 ± 0.036

5.561 ± 0.074

9.501 ± 0.119

3.452 ± 0.065

7.61 ± 0.09

1.798 ± 0.045

7.038 ± 0.075

7.378 ± 0.114

9.677 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.037

3.245 ± 0.057

4.237 ± 0.064

2.503 ± 0.041

5.882 ± 0.093

6.052 ± 0.082

4.921 ± 0.053

7.19 ± 0.079

1.082 ± 0.039

2.729 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski