Cronartium ribicola mitovirus 3
Average proteome isoelectric point is 7.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KCP3|A0A191KCP3_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 3 OX=1816486 GN=RdRp PE=4 SV=1
MM1 pKa = 7.7 NMNQNKK7 pKa = 9.85 AYY9 pKa = 9.89 SALVQAIFTFLIYY22 pKa = 9.81 YY23 pKa = 10.23 SPGFEE28 pKa = 3.92 SDD30 pKa = 3.6 KK31 pKa = 10.99 IVIILSDD38 pKa = 4.0 FKK40 pKa = 10.88 RR41 pKa = 11.84 HH42 pKa = 5.88 CDD44 pKa = 3.32 QRR46 pKa = 11.84 GIKK49 pKa = 10.25 AGISWIKK56 pKa = 10.27 DD57 pKa = 3.0 LRR59 pKa = 11.84 LCFTKK64 pKa = 10.93 YY65 pKa = 8.91 QAGDD69 pKa = 3.59 SKK71 pKa = 11.22 PKK73 pKa = 10.81 VNIKK77 pKa = 9.57 CHH79 pKa = 6.27 PNGCPRR85 pKa = 11.84 LIWDD89 pKa = 3.78 NYY91 pKa = 10.41 KK92 pKa = 10.88 DD93 pKa = 3.72 VLNDD97 pKa = 3.52 PKK99 pKa = 11.04 GIGIIITLLSISRR112 pKa = 11.84 PFEE115 pKa = 4.57 GIFEE119 pKa = 4.66 PSLSDD124 pKa = 3.18 ITDD127 pKa = 3.59 TPKK130 pKa = 10.55 TDD132 pKa = 3.44 LGIIKK137 pKa = 10.03 DD138 pKa = 3.9 LEE140 pKa = 4.17 EE141 pKa = 3.9 FLPAFFEE148 pKa = 4.61 EE149 pKa = 4.95 VVHH152 pKa = 5.84 VRR154 pKa = 11.84 KK155 pKa = 10.07 EE156 pKa = 3.89 SGQLKK161 pKa = 8.95 RR162 pKa = 11.84 PKK164 pKa = 9.39 WSSWLNNIKK173 pKa = 10.07 IAPNGVSINSSYY185 pKa = 11.81 ADD187 pKa = 3.48 LDD189 pKa = 3.72 LHH191 pKa = 7.4 NEE193 pKa = 4.04 EE194 pKa = 5.2 IIRR197 pKa = 11.84 DD198 pKa = 3.91 LFEE201 pKa = 6.35 LGGDD205 pKa = 3.96 CFEE208 pKa = 5.42 KK209 pKa = 10.34 EE210 pKa = 3.91 YY211 pKa = 11.46 SSIKK215 pKa = 10.47 DD216 pKa = 3.29 YY217 pKa = 11.04 WKK219 pKa = 10.73 DD220 pKa = 3.36 PEE222 pKa = 4.33 GQKK225 pKa = 10.58 EE226 pKa = 3.86 ITKK229 pKa = 10.31 NYY231 pKa = 9.43 PIVNTYY237 pKa = 8.83 KK238 pKa = 10.41 SKK240 pKa = 10.33 YY241 pKa = 8.94 LGRR244 pKa = 11.84 LAVIPSPEE252 pKa = 3.98 GKK254 pKa = 9.96 SRR256 pKa = 11.84 VIAIGDD262 pKa = 3.5 YY263 pKa = 9.59 WSQNVLFPLHH273 pKa = 6.31 NKK275 pKa = 9.41 IFGLLKK281 pKa = 10.49 RR282 pKa = 11.84 IPQDD286 pKa = 3.04 LTFAQEE292 pKa = 3.88 QGLSKK297 pKa = 10.25 IKK299 pKa = 10.12 KK300 pKa = 9.97 DD301 pKa = 3.43 RR302 pKa = 11.84 DD303 pKa = 3.19 HH304 pKa = 7.15 SYY306 pKa = 10.45 WSLDD310 pKa = 3.44 LKK312 pKa = 11.0 SATDD316 pKa = 3.74 RR317 pKa = 11.84 FPIEE321 pKa = 3.66 LQRR324 pKa = 11.84 RR325 pKa = 11.84 IISYY329 pKa = 9.95 IYY331 pKa = 9.91 GEE333 pKa = 4.59 SYY335 pKa = 10.56 SISWRR340 pKa = 11.84 NLMSRR345 pKa = 11.84 PFYY348 pKa = 9.87 QTISGKK354 pKa = 9.86 SEE356 pKa = 3.57 VTYY359 pKa = 10.61 GAGQPIGIYY368 pKa = 10.05 SSWSVFTLCHH378 pKa = 6.5 HH379 pKa = 6.57 FVLYY383 pKa = 10.1 CAQRR387 pKa = 11.84 RR388 pKa = 11.84 SIALKK393 pKa = 9.35 KK394 pKa = 9.92 GRR396 pKa = 11.84 YY397 pKa = 8.78 VILGDD402 pKa = 5.28 DD403 pKa = 4.11 IVISDD408 pKa = 4.43 DD409 pKa = 3.6 NLAIEE414 pKa = 4.24 YY415 pKa = 9.8 KK416 pKa = 10.65 KK417 pKa = 10.8 LINLLGVEE425 pKa = 3.93 ISEE428 pKa = 4.5 HH429 pKa = 5.4 KK430 pKa = 7.73 THH432 pKa = 6.93 KK433 pKa = 10.26 SKK435 pKa = 10.85 HH436 pKa = 5.69 SYY438 pKa = 10.09 EE439 pKa = 3.78 IAKK442 pKa = 10.03 RR443 pKa = 11.84 WITEE447 pKa = 3.86 KK448 pKa = 11.04 GEE450 pKa = 3.91 QSPFPIIPLIQSKK463 pKa = 9.72 SWVPAIVNTIIDD475 pKa = 4.07 AEE477 pKa = 4.32 EE478 pKa = 4.35 KK479 pKa = 10.59 GWVPSCSTFKK489 pKa = 10.4 IAAIIAMLKK498 pKa = 10.15 RR499 pKa = 11.84 KK500 pKa = 9.7 SSNQGLWNHH509 pKa = 6.51 IYY511 pKa = 9.6 KK512 pKa = 10.74 LSMIHH517 pKa = 6.06 SSCYY521 pKa = 10.13 LAFKK525 pKa = 10.74 DD526 pKa = 4.81 KK527 pKa = 10.39 ISNMDD532 pKa = 4.92 CINMLTDD539 pKa = 3.89 AFQIEE544 pKa = 4.42 RR545 pKa = 11.84 LSQNEE550 pKa = 3.78 VSASILIAQASKK562 pKa = 11.25 NILATLGKK570 pKa = 10.18 EE571 pKa = 3.94 IFKK574 pKa = 9.5 STLFQLPEE582 pKa = 4.36 EE583 pKa = 4.12 IQKK586 pKa = 10.32 VKK588 pKa = 10.74 EE589 pKa = 3.9 LVDD592 pKa = 4.15 FEE594 pKa = 4.28 EE595 pKa = 4.89 TTWSKK600 pKa = 11.26 YY601 pKa = 10.33 SPQEE605 pKa = 3.69 HH606 pKa = 6.7 EE607 pKa = 3.9 MTEE610 pKa = 4.16 RR611 pKa = 11.84 LPHH614 pKa = 6.57 CDD616 pKa = 3.38 VLRR619 pKa = 11.84 KK620 pKa = 9.47 ICGKK624 pKa = 9.83 IFEE627 pKa = 5.28 PDD629 pKa = 3.07 FPGIQDD635 pKa = 3.67 EE636 pKa = 4.85 PDD638 pKa = 3.48 PKK640 pKa = 10.29 SWKK643 pKa = 8.33 RR644 pKa = 11.84 TIRR647 pKa = 11.84 GLISFDD653 pKa = 3.44 VKK655 pKa = 10.85 KK656 pKa = 11.25 VFIKK660 pKa = 10.47 DD661 pKa = 3.37 KK662 pKa = 10.71 KK663 pKa = 10.05 RR664 pKa = 11.84 VEE666 pKa = 3.77 ILQVSKK672 pKa = 11.01 VSKK675 pKa = 9.84 SLCGIIKK682 pKa = 10.15 SLNNMEE688 pKa = 4.36 EE689 pKa = 5.26 KK690 pKa = 10.47 EE691 pKa = 4.52 LEE693 pKa = 4.11 WTNLKK698 pKa = 10.19 PGSSSLTSLNYY709 pKa = 10.66 LFFPP713 pKa = 5.28
Molecular weight: 81.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.719
IPC2_protein 7.995
IPC_protein 7.805
Toseland 7.834
ProMoST 8.434
Dawson 8.682
Bjellqvist 8.814
Wikipedia 8.682
Rodwell 8.785
Grimsley 7.863
Solomon 8.741
Lehninger 8.741
Nozaki 8.99
DTASelect 8.624
Thurlkill 8.726
EMBOSS 8.873
Sillero 8.975
Patrickios 4.52
IPC_peptide 8.741
IPC2_peptide 7.497
IPC2.peptide.svr19 7.793
Protein with the highest isoelectric point:
>tr|A0A191KCP3|A0A191KCP3_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 3 OX=1816486 GN=RdRp PE=4 SV=1
MM1 pKa = 7.7 NMNQNKK7 pKa = 9.85 AYY9 pKa = 9.89 SALVQAIFTFLIYY22 pKa = 9.81 YY23 pKa = 10.23 SPGFEE28 pKa = 3.92 SDD30 pKa = 3.6 KK31 pKa = 10.99 IVIILSDD38 pKa = 4.0 FKK40 pKa = 10.88 RR41 pKa = 11.84 HH42 pKa = 5.88 CDD44 pKa = 3.32 QRR46 pKa = 11.84 GIKK49 pKa = 10.25 AGISWIKK56 pKa = 10.27 DD57 pKa = 3.0 LRR59 pKa = 11.84 LCFTKK64 pKa = 10.93 YY65 pKa = 8.91 QAGDD69 pKa = 3.59 SKK71 pKa = 11.22 PKK73 pKa = 10.81 VNIKK77 pKa = 9.57 CHH79 pKa = 6.27 PNGCPRR85 pKa = 11.84 LIWDD89 pKa = 3.78 NYY91 pKa = 10.41 KK92 pKa = 10.88 DD93 pKa = 3.72 VLNDD97 pKa = 3.52 PKK99 pKa = 11.04 GIGIIITLLSISRR112 pKa = 11.84 PFEE115 pKa = 4.57 GIFEE119 pKa = 4.66 PSLSDD124 pKa = 3.18 ITDD127 pKa = 3.59 TPKK130 pKa = 10.55 TDD132 pKa = 3.44 LGIIKK137 pKa = 10.03 DD138 pKa = 3.9 LEE140 pKa = 4.17 EE141 pKa = 3.9 FLPAFFEE148 pKa = 4.61 EE149 pKa = 4.95 VVHH152 pKa = 5.84 VRR154 pKa = 11.84 KK155 pKa = 10.07 EE156 pKa = 3.89 SGQLKK161 pKa = 8.95 RR162 pKa = 11.84 PKK164 pKa = 9.39 WSSWLNNIKK173 pKa = 10.07 IAPNGVSINSSYY185 pKa = 11.81 ADD187 pKa = 3.48 LDD189 pKa = 3.72 LHH191 pKa = 7.4 NEE193 pKa = 4.04 EE194 pKa = 5.2 IIRR197 pKa = 11.84 DD198 pKa = 3.91 LFEE201 pKa = 6.35 LGGDD205 pKa = 3.96 CFEE208 pKa = 5.42 KK209 pKa = 10.34 EE210 pKa = 3.91 YY211 pKa = 11.46 SSIKK215 pKa = 10.47 DD216 pKa = 3.29 YY217 pKa = 11.04 WKK219 pKa = 10.73 DD220 pKa = 3.36 PEE222 pKa = 4.33 GQKK225 pKa = 10.58 EE226 pKa = 3.86 ITKK229 pKa = 10.31 NYY231 pKa = 9.43 PIVNTYY237 pKa = 8.83 KK238 pKa = 10.41 SKK240 pKa = 10.33 YY241 pKa = 8.94 LGRR244 pKa = 11.84 LAVIPSPEE252 pKa = 3.98 GKK254 pKa = 9.96 SRR256 pKa = 11.84 VIAIGDD262 pKa = 3.5 YY263 pKa = 9.59 WSQNVLFPLHH273 pKa = 6.31 NKK275 pKa = 9.41 IFGLLKK281 pKa = 10.49 RR282 pKa = 11.84 IPQDD286 pKa = 3.04 LTFAQEE292 pKa = 3.88 QGLSKK297 pKa = 10.25 IKK299 pKa = 10.12 KK300 pKa = 9.97 DD301 pKa = 3.43 RR302 pKa = 11.84 DD303 pKa = 3.19 HH304 pKa = 7.15 SYY306 pKa = 10.45 WSLDD310 pKa = 3.44 LKK312 pKa = 11.0 SATDD316 pKa = 3.74 RR317 pKa = 11.84 FPIEE321 pKa = 3.66 LQRR324 pKa = 11.84 RR325 pKa = 11.84 IISYY329 pKa = 9.95 IYY331 pKa = 9.91 GEE333 pKa = 4.59 SYY335 pKa = 10.56 SISWRR340 pKa = 11.84 NLMSRR345 pKa = 11.84 PFYY348 pKa = 9.87 QTISGKK354 pKa = 9.86 SEE356 pKa = 3.57 VTYY359 pKa = 10.61 GAGQPIGIYY368 pKa = 10.05 SSWSVFTLCHH378 pKa = 6.5 HH379 pKa = 6.57 FVLYY383 pKa = 10.1 CAQRR387 pKa = 11.84 RR388 pKa = 11.84 SIALKK393 pKa = 9.35 KK394 pKa = 9.92 GRR396 pKa = 11.84 YY397 pKa = 8.78 VILGDD402 pKa = 5.28 DD403 pKa = 4.11 IVISDD408 pKa = 4.43 DD409 pKa = 3.6 NLAIEE414 pKa = 4.24 YY415 pKa = 9.8 KK416 pKa = 10.65 KK417 pKa = 10.8 LINLLGVEE425 pKa = 3.93 ISEE428 pKa = 4.5 HH429 pKa = 5.4 KK430 pKa = 7.73 THH432 pKa = 6.93 KK433 pKa = 10.26 SKK435 pKa = 10.85 HH436 pKa = 5.69 SYY438 pKa = 10.09 EE439 pKa = 3.78 IAKK442 pKa = 10.03 RR443 pKa = 11.84 WITEE447 pKa = 3.86 KK448 pKa = 11.04 GEE450 pKa = 3.91 QSPFPIIPLIQSKK463 pKa = 9.72 SWVPAIVNTIIDD475 pKa = 4.07 AEE477 pKa = 4.32 EE478 pKa = 4.35 KK479 pKa = 10.59 GWVPSCSTFKK489 pKa = 10.4 IAAIIAMLKK498 pKa = 10.15 RR499 pKa = 11.84 KK500 pKa = 9.7 SSNQGLWNHH509 pKa = 6.51 IYY511 pKa = 9.6 KK512 pKa = 10.74 LSMIHH517 pKa = 6.06 SSCYY521 pKa = 10.13 LAFKK525 pKa = 10.74 DD526 pKa = 4.81 KK527 pKa = 10.39 ISNMDD532 pKa = 4.92 CINMLTDD539 pKa = 3.89 AFQIEE544 pKa = 4.42 RR545 pKa = 11.84 LSQNEE550 pKa = 3.78 VSASILIAQASKK562 pKa = 11.25 NILATLGKK570 pKa = 10.18 EE571 pKa = 3.94 IFKK574 pKa = 9.5 STLFQLPEE582 pKa = 4.36 EE583 pKa = 4.12 IQKK586 pKa = 10.32 VKK588 pKa = 10.74 EE589 pKa = 3.9 LVDD592 pKa = 4.15 FEE594 pKa = 4.28 EE595 pKa = 4.89 TTWSKK600 pKa = 11.26 YY601 pKa = 10.33 SPQEE605 pKa = 3.69 HH606 pKa = 6.7 EE607 pKa = 3.9 MTEE610 pKa = 4.16 RR611 pKa = 11.84 LPHH614 pKa = 6.57 CDD616 pKa = 3.38 VLRR619 pKa = 11.84 KK620 pKa = 9.47 ICGKK624 pKa = 9.83 IFEE627 pKa = 5.28 PDD629 pKa = 3.07 FPGIQDD635 pKa = 3.67 EE636 pKa = 4.85 PDD638 pKa = 3.48 PKK640 pKa = 10.29 SWKK643 pKa = 8.33 RR644 pKa = 11.84 TIRR647 pKa = 11.84 GLISFDD653 pKa = 3.44 VKK655 pKa = 10.85 KK656 pKa = 11.25 VFIKK660 pKa = 10.47 DD661 pKa = 3.37 KK662 pKa = 10.71 KK663 pKa = 10.05 RR664 pKa = 11.84 VEE666 pKa = 3.77 ILQVSKK672 pKa = 11.01 VSKK675 pKa = 9.84 SLCGIIKK682 pKa = 10.15 SLNNMEE688 pKa = 4.36 EE689 pKa = 5.26 KK690 pKa = 10.47 EE691 pKa = 4.52 LEE693 pKa = 4.11 WTNLKK698 pKa = 10.19 PGSSSLTSLNYY709 pKa = 10.66 LFFPP713 pKa = 5.28
Molecular weight: 81.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.719
IPC2_protein 7.995
IPC_protein 7.805
Toseland 7.834
ProMoST 8.434
Dawson 8.682
Bjellqvist 8.814
Wikipedia 8.682
Rodwell 8.785
Grimsley 7.863
Solomon 8.741
Lehninger 8.741
Nozaki 8.99
DTASelect 8.624
Thurlkill 8.726
EMBOSS 8.873
Sillero 8.975
Patrickios 4.52
IPC_peptide 8.741
IPC2_peptide 7.497
IPC2.peptide.svr19 7.793
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
713
713
713
713.0
81.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.927 ± 0.0
1.823 ± 0.0
5.47 ± 0.0
6.452 ± 0.0
4.488 ± 0.0
5.049 ± 0.0
2.104 ± 0.0
10.94 ± 0.0
9.677 ± 0.0
8.836 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.262 ± 0.0
4.208 ± 0.0
4.769 ± 0.0
3.506 ± 0.0
3.927 ± 0.0
9.677 ± 0.0
3.647 ± 0.0
4.208 ± 0.0
2.244 ± 0.0
3.787 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here