Hubei noda-like virus 8
Average proteome isoelectric point is 7.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGR5|A0A1L3KGR5_9VIRU RNA replicase OS=Hubei noda-like virus 8 OX=1922988 PE=3 SV=1
MM1 pKa = 7.31 SRR3 pKa = 11.84 ISHH6 pKa = 7.1 FLTGVTYY13 pKa = 9.19 PYY15 pKa = 10.99 GCGRR19 pKa = 11.84 PSPFDD24 pKa = 3.44 VEE26 pKa = 4.11 PRR28 pKa = 11.84 SWNMPYY34 pKa = 10.61 RR35 pKa = 11.84 LAKK38 pKa = 9.57 ACCYY42 pKa = 9.27 LAKK45 pKa = 10.7 HH46 pKa = 5.21 NVEE49 pKa = 4.54 LLSGQNIRR57 pKa = 11.84 DD58 pKa = 3.51 ILSKK62 pKa = 10.76 FKK64 pKa = 10.41 TFYY67 pKa = 10.99 EE68 pKa = 4.67 CVTKK72 pKa = 10.56 LWADD76 pKa = 4.93 GITLEE81 pKa = 4.51 CAIEE85 pKa = 4.28 TVVSSTNGLYY95 pKa = 10.17 LRR97 pKa = 11.84 KK98 pKa = 8.57 PTLKK102 pKa = 10.24 EE103 pKa = 2.95 IRR105 pKa = 11.84 YY106 pKa = 8.74 VVKK109 pKa = 10.67 LYY111 pKa = 11.21 LEE113 pKa = 4.24 ALFLIITVPPLTQVQIASLLFDD135 pKa = 5.4 SIASYY140 pKa = 11.26 YY141 pKa = 9.91 GIKK144 pKa = 10.49 APANRR149 pKa = 11.84 HH150 pKa = 4.45 SFFVEE155 pKa = 4.32 VTSDD159 pKa = 3.69 EE160 pKa = 4.41 PVLDD164 pKa = 3.62 TRR166 pKa = 11.84 RR167 pKa = 11.84 EE168 pKa = 3.89 LMLDD172 pKa = 3.31 VVPIRR177 pKa = 11.84 RR178 pKa = 11.84 TMSNHH183 pKa = 5.0 SHH185 pKa = 7.0 PSSAAEE191 pKa = 3.68 RR192 pKa = 11.84 SSVHH196 pKa = 5.56 AAIVNKK202 pKa = 9.86 IVEE205 pKa = 4.29 AGYY208 pKa = 10.76 EE209 pKa = 3.95 PFDD212 pKa = 3.51 ISMSKK217 pKa = 10.28 SAIEE221 pKa = 3.94 QGLSGQRR228 pKa = 11.84 YY229 pKa = 7.78 FYY231 pKa = 9.8 WLKK234 pKa = 10.6 DD235 pKa = 3.68 YY236 pKa = 10.59 KK237 pKa = 10.7 CSVVEE242 pKa = 3.73 HH243 pKa = 7.14 PIRR246 pKa = 11.84 HH247 pKa = 5.63 SSVIVMVDD255 pKa = 2.23 VDD257 pKa = 4.39 YY258 pKa = 11.64 YY259 pKa = 11.74 LDD261 pKa = 3.28 INEE264 pKa = 3.92 YY265 pKa = 8.74 MKK267 pKa = 10.61 YY268 pKa = 10.03 FRR270 pKa = 11.84 PIVIYY275 pKa = 8.93 TLQPLTLRR283 pKa = 11.84 FVGPEE288 pKa = 3.37 YY289 pKa = 10.82 SFRR292 pKa = 11.84 VDD294 pKa = 2.92 KK295 pKa = 11.67 GEE297 pKa = 3.58 VFYY300 pKa = 10.45 EE301 pKa = 3.91 VRR303 pKa = 11.84 GGAKK307 pKa = 9.77 YY308 pKa = 9.59 SHH310 pKa = 6.98 KK311 pKa = 9.08 IWNYY315 pKa = 7.08 STHH318 pKa = 6.59 GDD320 pKa = 3.7 TVSVRR325 pKa = 11.84 DD326 pKa = 3.77 WYY328 pKa = 11.31 GNQCVFSVEE337 pKa = 3.97 KK338 pKa = 10.68 QLVGKK343 pKa = 9.13 PDD345 pKa = 3.64 EE346 pKa = 4.72 GSSRR350 pKa = 11.84 HH351 pKa = 6.05 IIYY354 pKa = 10.29 LNPIRR359 pKa = 11.84 RR360 pKa = 11.84 IPWFCITEE368 pKa = 3.73 KK369 pKa = 10.98 DD370 pKa = 3.75 MPLVRR375 pKa = 11.84 HH376 pKa = 7.06 DD377 pKa = 3.84 YY378 pKa = 9.54 TLSTRR383 pKa = 11.84 RR384 pKa = 11.84 PLVEE388 pKa = 4.26 TPSITTTTTEE398 pKa = 4.18 KK399 pKa = 10.94 KK400 pKa = 10.04 PDD402 pKa = 3.67 GTVKK406 pKa = 10.32 TITNKK411 pKa = 9.06 TKK413 pKa = 10.37 QKK415 pKa = 10.22 EE416 pKa = 4.41 SVIEE420 pKa = 3.94 KK421 pKa = 10.08 LGNSLWDD428 pKa = 3.39 SFNYY432 pKa = 9.7 GVSEE436 pKa = 4.03 GFNYY440 pKa = 10.15 ILDD443 pKa = 5.08 PIDD446 pKa = 4.04 NYY448 pKa = 10.73 IEE450 pKa = 4.1 LAEE453 pKa = 4.57 SNSFTSMRR461 pKa = 11.84 LTIPEE466 pKa = 4.17 FEE468 pKa = 4.43 SFLTRR473 pKa = 11.84 AEE475 pKa = 4.14 IKK477 pKa = 9.68 PLKK480 pKa = 10.16 PGDD483 pKa = 4.02 IEE485 pKa = 4.4 LQLKK489 pKa = 9.54 KK490 pKa = 10.39 RR491 pKa = 11.84 YY492 pKa = 8.79 SEE494 pKa = 4.31 KK495 pKa = 10.5 KK496 pKa = 9.66 GEE498 pKa = 3.97 AMPYY502 pKa = 9.73 EE503 pKa = 4.95 EE504 pKa = 4.91 IVKK507 pKa = 9.06 TPIIAKK513 pKa = 9.67 ILSNVGKK520 pKa = 7.45 TVKK523 pKa = 10.79 GNTTPTNYY531 pKa = 9.86 IQMKK535 pKa = 9.9 QSTTVEE541 pKa = 4.16 TANLDD546 pKa = 3.73 SQVFTVEE553 pKa = 3.81 GKK555 pKa = 10.4 DD556 pKa = 3.01 IFFEE560 pKa = 4.57 SKK562 pKa = 10.88 LPGQVITSCLVRR574 pKa = 11.84 IPAVLPSRR582 pKa = 11.84 TRR584 pKa = 11.84 NNNQASIMGRR594 pKa = 11.84 ISEE597 pKa = 4.21 LHH599 pKa = 6.26 NDD601 pKa = 3.63 VVPTVLNHH609 pKa = 6.63 LRR611 pKa = 11.84 FNSAATEE618 pKa = 4.41 FVRR621 pKa = 11.84 LMVPEE626 pKa = 4.74 IGHH629 pKa = 5.36 GVEE632 pKa = 4.02 YY633 pKa = 10.9 SIDD636 pKa = 3.62 QVVAKK641 pKa = 9.9 QDD643 pKa = 2.98 KK644 pKa = 8.75 CAQRR648 pKa = 11.84 QRR650 pKa = 11.84 INEE653 pKa = 4.25 IINTFSAEE661 pKa = 3.82 PTNRR665 pKa = 11.84 LTTFIKK671 pKa = 10.44 NEE673 pKa = 3.86 AYY675 pKa = 10.42 ASVNDD680 pKa = 3.84 PRR682 pKa = 11.84 NITTMSSNFTLIASKK697 pKa = 8.84 ITYY700 pKa = 9.34 AFKK703 pKa = 10.8 DD704 pKa = 3.52 AVLKK708 pKa = 7.44 QHH710 pKa = 5.91 KK711 pKa = 9.21 WYY713 pKa = 10.73 GPGKK717 pKa = 7.98 TPSEE721 pKa = 4.29 SVDD724 pKa = 3.51 LLRR727 pKa = 11.84 NICANGACLTDD738 pKa = 3.12 YY739 pKa = 10.96 SRR741 pKa = 11.84 YY742 pKa = 9.87 DD743 pKa = 3.27 GRR745 pKa = 11.84 VSKK748 pKa = 10.12 WLQNRR753 pKa = 11.84 IAKK756 pKa = 9.53 AAYY759 pKa = 9.49 LRR761 pKa = 11.84 WASPGDD767 pKa = 3.33 TKK769 pKa = 11.5 EE770 pKa = 4.34 NVNHH774 pKa = 5.61 MFKK777 pKa = 10.61 EE778 pKa = 4.13 IFRR781 pKa = 11.84 LTGRR785 pKa = 11.84 TADD788 pKa = 3.5 GEE790 pKa = 4.41 LYY792 pKa = 10.54 YY793 pKa = 10.64 AGHH796 pKa = 6.07 GTRR799 pKa = 11.84 SGSPITTDD807 pKa = 3.04 ANTMINAYY815 pKa = 9.47 IAFCALLYY823 pKa = 10.57 LGYY826 pKa = 10.43 DD827 pKa = 3.51 SKK829 pKa = 10.31 TSFALLGLYY838 pKa = 10.43 AGDD841 pKa = 5.04 DD842 pKa = 3.71 GVTPYY847 pKa = 10.56 IPGIEE852 pKa = 3.87 TALYY856 pKa = 9.58 KK857 pKa = 10.75 VCTALGMVVKK867 pKa = 10.69 LEE869 pKa = 3.85 VSPIGEE875 pKa = 4.41 EE876 pKa = 3.56 INYY879 pKa = 9.88 LGRR882 pKa = 11.84 IFPNILVSDD891 pKa = 4.16 ISYY894 pKa = 10.98 QDD896 pKa = 3.39 IKK898 pKa = 10.63 RR899 pKa = 11.84 TLPKK903 pKa = 10.54 LHH905 pKa = 7.07 ISTNQQVKK913 pKa = 10.31 RR914 pKa = 11.84 EE915 pKa = 3.95 VAAVNKK921 pKa = 10.23 ALGYY925 pKa = 8.39 LTTDD929 pKa = 4.66 AITPIIGSWAKK940 pKa = 10.05 RR941 pKa = 11.84 VLDD944 pKa = 4.41 LNPDD948 pKa = 3.57 VACRR952 pKa = 11.84 NFLSEE957 pKa = 4.35 EE958 pKa = 3.94 LFKK961 pKa = 10.77 IEE963 pKa = 4.26 NGAWPQPTQPEE974 pKa = 4.2 DD975 pKa = 3.4 LDD977 pKa = 5.37 LIVEE981 pKa = 4.67 SICKK985 pKa = 9.5 HH986 pKa = 6.73 LDD988 pKa = 3.23 MTPSEE993 pKa = 4.56 LEE995 pKa = 4.32 CFDD998 pKa = 3.59 EE999 pKa = 6.44 AIRR1002 pKa = 11.84 TSDD1005 pKa = 3.56 TLCTHH1010 pKa = 7.33 LDD1012 pKa = 3.42 YY1013 pKa = 11.32 FDD1015 pKa = 4.57 IVWEE1019 pKa = 4.15 NEE1021 pKa = 4.08 VEE1023 pKa = 4.48 HH1024 pKa = 6.81 KK1025 pKa = 10.66 RR1026 pKa = 11.84 PAYY1029 pKa = 10.54 LNGEE1033 pKa = 4.25 IVGTGQPSYY1042 pKa = 10.56 TVGLTPATNINQCQINQLITPPPPSKK1068 pKa = 10.1 IKK1070 pKa = 10.46 KK1071 pKa = 8.98 EE1072 pKa = 3.98 KK1073 pKa = 9.7 EE1074 pKa = 3.87 KK1075 pKa = 11.06 KK1076 pKa = 10.18 KK1077 pKa = 10.46 NASNKK1082 pKa = 9.62 KK1083 pKa = 8.25 KK1084 pKa = 9.38 QQSSGSISPEE1094 pKa = 3.57 PVAVPSTSEE1103 pKa = 4.6 DD1104 pKa = 3.61 FPQIATHH1111 pKa = 5.87 SRR1113 pKa = 11.84 SGTGSISSSSDD1124 pKa = 2.85 KK1125 pKa = 10.04 QTTSSSPKK1133 pKa = 9.63 RR1134 pKa = 11.84 EE1135 pKa = 3.68 RR1136 pKa = 11.84 KK1137 pKa = 9.55 LSPKK1141 pKa = 8.06 STKK1144 pKa = 9.76 KK1145 pKa = 10.37 RR1146 pKa = 11.84 SEE1148 pKa = 3.74 KK1149 pKa = 10.66 SKK1151 pKa = 10.98 ASRR1154 pKa = 11.84 TLSTPSTT1161 pKa = 3.77
Molecular weight: 130.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.444
IPC2_protein 7.834
IPC_protein 7.629
Toseland 7.38
ProMoST 8.229
Dawson 8.419
Bjellqvist 8.668
Wikipedia 8.375
Rodwell 8.463
Grimsley 7.439
Solomon 8.448
Lehninger 8.448
Nozaki 8.785
DTASelect 8.448
Thurlkill 8.507
EMBOSS 8.595
Sillero 8.785
Patrickios 4.266
IPC_peptide 8.448
IPC2_peptide 7.468
IPC2.peptide.svr19 7.826
Protein with the highest isoelectric point:
>tr|A0A1L3KGR5|A0A1L3KGR5_9VIRU RNA replicase OS=Hubei noda-like virus 8 OX=1922988 PE=3 SV=1
MM1 pKa = 7.67 PQQQNNTPALNVNLNTNGRR20 pKa = 11.84 PSRR23 pKa = 11.84 SRR25 pKa = 11.84 SRR27 pKa = 11.84 KK28 pKa = 8.58 KK29 pKa = 9.92 RR30 pKa = 11.84 SKK32 pKa = 10.16 RR33 pKa = 11.84 SQSRR37 pKa = 11.84 SASNVIRR44 pKa = 11.84 NRR46 pKa = 11.84 VMLGKK51 pKa = 10.12 NYY53 pKa = 9.72 TGNPLPGDD61 pKa = 3.77 VLFALPPNATRR72 pKa = 11.84 SGFKK76 pKa = 10.19 PNPADD81 pKa = 3.68 PRR83 pKa = 11.84 TFDD86 pKa = 4.07 AFTNLGNTSTGRR98 pKa = 11.84 AAAQKK103 pKa = 9.8 ILHH106 pKa = 6.28 PCEE109 pKa = 4.68 EE110 pKa = 4.41 GAQPFVRR117 pKa = 11.84 FPDD120 pKa = 3.95 GAASITTAFEE130 pKa = 3.84 RR131 pKa = 11.84 RR132 pKa = 11.84 DD133 pKa = 3.25 EE134 pKa = 4.24 YY135 pKa = 10.8 MISRR139 pKa = 11.84 QYY141 pKa = 9.89 TADD144 pKa = 3.22 SNMNILVVHH153 pKa = 6.37 LPFLRR158 pKa = 11.84 YY159 pKa = 9.23 NRR161 pKa = 11.84 LVIQWDD167 pKa = 4.07 SSQSPTDD174 pKa = 3.53 YY175 pKa = 11.31 DD176 pKa = 3.71 LQTVFTEE183 pKa = 4.05 ALTGVAIYY191 pKa = 9.0 PSWNTKK197 pKa = 5.9 TTGDD201 pKa = 3.38 TSDD204 pKa = 3.32 QITYY208 pKa = 8.01 YY209 pKa = 8.07 TTILSPNVLTPGGGGTPEE227 pKa = 3.87 AARR230 pKa = 11.84 SYY232 pKa = 10.1 LRR234 pKa = 11.84 NVRR237 pKa = 11.84 RR238 pKa = 11.84 VYY240 pKa = 10.75 YY241 pKa = 10.73 GSTQDD246 pKa = 4.52 LDD248 pKa = 3.79 ASSLYY253 pKa = 10.85 DD254 pKa = 3.29 EE255 pKa = 4.92 GRR257 pKa = 11.84 VVAGQWSPDD266 pKa = 3.17 VVLGINRR273 pKa = 11.84 ARR275 pKa = 11.84 DD276 pKa = 3.46 NTVQNSCYY284 pKa = 9.64 RR285 pKa = 11.84 LSVPALTSEE294 pKa = 5.46 SIVQSDD300 pKa = 4.04 AYY302 pKa = 10.39 RR303 pKa = 11.84 YY304 pKa = 8.68 QALAKK309 pKa = 8.37 TGCYY313 pKa = 8.95 MPLRR317 pKa = 11.84 PSDD320 pKa = 3.97 PDD322 pKa = 3.54 VQFSGASEE330 pKa = 3.86 QRR332 pKa = 11.84 PLEE335 pKa = 4.15 IAFHH339 pKa = 5.53 QSGNITGNLTTVDD352 pKa = 4.75 DD353 pKa = 3.25 IWLRR357 pKa = 11.84 GWNIGVEE364 pKa = 4.06 LWTNIDD370 pKa = 3.0 YY371 pKa = 10.29 RR372 pKa = 11.84 ANLRR376 pKa = 11.84 YY377 pKa = 7.98 KK378 pKa = 8.85 TCEE381 pKa = 3.83 GLEE384 pKa = 4.21 LSPSANSLYY393 pKa = 10.07 STFEE397 pKa = 3.57 GRR399 pKa = 11.84 GYY401 pKa = 10.13 PRR403 pKa = 11.84 DD404 pKa = 3.82 DD405 pKa = 3.5 RR406 pKa = 11.84 ALSVIRR412 pKa = 11.84 EE413 pKa = 4.17 FARR416 pKa = 11.84 SQPHH420 pKa = 7.47 AYY422 pKa = 9.15 PADD425 pKa = 4.08 FNDD428 pKa = 5.34 KK429 pKa = 10.69 NLLWGQLIRR438 pKa = 11.84 GLGTALSSLGLPILSQVAEE457 pKa = 4.48 PVAHH461 pKa = 7.4 AIANYY466 pKa = 8.51 VEE468 pKa = 4.21 RR469 pKa = 5.89
Molecular weight: 52.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.382
IPC2_protein 8.419
IPC_protein 8.478
Toseland 8.507
ProMoST 8.829
Dawson 8.975
Bjellqvist 9.004
Wikipedia 9.165
Rodwell 8.99
Grimsley 8.902
Solomon 9.092
Lehninger 9.033
Nozaki 8.931
DTASelect 8.887
Thurlkill 8.873
EMBOSS 9.077
Sillero 9.092
Patrickios 4.279
IPC_peptide 9.063
IPC2_peptide 7.995
IPC2.peptide.svr19 7.92
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1630
469
1161
815.0
91.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.258 ± 0.893
1.411 ± 0.27
4.847 ± 0.337
5.583 ± 0.948
3.313 ± 0.159
5.583 ± 0.6
1.718 ± 0.315
6.503 ± 1.083
5.583 ± 1.67
8.037 ± 0.341
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.411 ± 0.167
5.337 ± 0.719
6.012 ± 0.289
3.436 ± 0.607
6.012 ± 1.115
8.896 ± 0.029
7.73 ± 0.129
6.503 ± 0.361
1.227 ± 0.129
4.601 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here