Amino acid dipepetide frequency for Trifolium medium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.049AlaAla: 6.049 ± 0.033	1.087AlaCys: 1.087 ± 0.01	3.299AlaAsp: 3.299 ± 0.016	4.529AlaGlu: 4.529 ± 0.021	2.401AlaPhe: 2.401 ± 0.015	3.901AlaGly: 3.901 ± 0.02	1.289AlaHis: 1.289 ± 0.01	3.459AlaIle: 3.459 ± 0.019	3.988AlaLys: 3.988 ± 0.019	5.975AlaLeu: 5.975 ± 0.022	2.01AlaMet: 2.01 ± 0.014	2.727AlaAsn: 2.727 ± 0.015	3.07AlaPro: 3.07 ± 0.017	2.62AlaGln: 2.62 ± 0.017	3.861AlaArg: 3.861 ± 0.02	5.538AlaSer: 5.538 ± 0.026	3.772AlaThr: 3.772 ± 0.022	4.686AlaVal: 4.686 ± 0.018	0.858AlaTrp: 0.858 ± 0.009	1.616AlaTyr: 1.616 ± 0.012	0.052AlaXaa: 0.052 ± 0.002
Cys	1.048CysAla: 1.048 ± 0.01	0.516CysCys: 0.516 ± 0.007	0.977CysAsp: 0.977 ± 0.008	0.895CysGlu: 0.895 ± 0.009	0.92CysPhe: 0.92 ± 0.011	1.322CysGly: 1.322 ± 0.011	0.465CysHis: 0.465 ± 0.006	0.853CysIle: 0.853 ± 0.01	1.064CysLys: 1.064 ± 0.012	1.631CysLeu: 1.631 ± 0.012	0.427CysMet: 0.427 ± 0.005	0.802CysAsn: 0.802 ± 0.009	0.938CysPro: 0.938 ± 0.008	0.642CysGln: 0.642 ± 0.008	0.993CysArg: 0.993 ± 0.009	1.592CysSer: 1.592 ± 0.013	0.746CysThr: 0.746 ± 0.007	1.197CysVal: 1.197 ± 0.01	0.325CysTrp: 0.325 ± 0.005	0.547CysTyr: 0.547 ± 0.007	0.011CysXaa: 0.011 ± 0.001
Asp	3.651AspAla: 3.651 ± 0.017	0.927AspCys: 0.927 ± 0.009	4.281AspAsp: 4.281 ± 0.028	4.675AspGlu: 4.675 ± 0.024	2.468AspPhe: 2.468 ± 0.015	4.067AspGly: 4.067 ± 0.018	1.24AspHis: 1.24 ± 0.01	3.112AspIle: 3.112 ± 0.017	3.029AspLys: 3.029 ± 0.018	4.963AspLeu: 4.963 ± 0.022	1.44AspMet: 1.44 ± 0.012	2.317AspAsn: 2.317 ± 0.014	2.567AspPro: 2.567 ± 0.015	1.741AspGln: 1.741 ± 0.012	2.484AspArg: 2.484 ± 0.018	4.082AspSer: 4.082 ± 0.019	2.286AspThr: 2.286 ± 0.015	4.239AspVal: 4.239 ± 0.02	0.771AspTrp: 0.771 ± 0.008	1.476AspTyr: 1.476 ± 0.013	0.04AspXaa: 0.04 ± 0.002
Glu	5.229GluAla: 5.229 ± 0.021	0.971GluCys: 0.971 ± 0.009	4.827GluAsp: 4.827 ± 0.021	7.924GluGlu: 7.924 ± 0.043	2.608GluPhe: 2.608 ± 0.015	4.359GluGly: 4.359 ± 0.017	1.358GluHis: 1.358 ± 0.01	3.902GluIle: 3.902 ± 0.019	5.112GluLys: 5.112 ± 0.024	6.155GluLeu: 6.155 ± 0.025	2.015GluMet: 2.015 ± 0.015	3.232GluAsn: 3.232 ± 0.018	2.328GluPro: 2.328 ± 0.015	2.382GluGln: 2.382 ± 0.016	3.526GluArg: 3.526 ± 0.018	4.6GluSer: 4.6 ± 0.02	3.312GluThr: 3.312 ± 0.017	5.099GluVal: 5.099 ± 0.022	0.803GluTrp: 0.803 ± 0.008	1.646GluTyr: 1.646 ± 0.014	0.047GluXaa: 0.047 ± 0.002
Phe	2.312PheAla: 2.312 ± 0.014	0.817PheCys: 0.817 ± 0.01	2.22PheAsp: 2.22 ± 0.015	2.574PheGlu: 2.574 ± 0.016	1.569PhePhe: 1.569 ± 0.013	2.651PheGly: 2.651 ± 0.015	1.077PheHis: 1.077 ± 0.011	1.913PheIle: 1.913 ± 0.013	2.307PheLys: 2.307 ± 0.014	3.724PheLeu: 3.724 ± 0.017	1.022PheMet: 1.022 ± 0.01	1.736PheAsn: 1.736 ± 0.012	1.998PhePro: 1.998 ± 0.013	1.467PheGln: 1.467 ± 0.011	1.91PheArg: 1.91 ± 0.014	3.377PheSer: 3.377 ± 0.019	1.923PheThr: 1.923 ± 0.013	2.745PheVal: 2.745 ± 0.017	0.652PheTrp: 0.652 ± 0.007	1.03PheTyr: 1.03 ± 0.01	0.029PheXaa: 0.029 ± 0.001
Gly	4.063GlyAla: 4.063 ± 0.019	1.279GlyCys: 1.279 ± 0.011	3.615GlyAsp: 3.615 ± 0.018	4.113GlyGlu: 4.113 ± 0.022	2.881GlyPhe: 2.881 ± 0.016	6.131GlyGly: 6.131 ± 0.043	1.613GlyHis: 1.613 ± 0.013	3.267GlyIle: 3.267 ± 0.017	4.174GlyLys: 4.174 ± 0.019	5.352GlyLeu: 5.352 ± 0.024	1.721GlyMet: 1.721 ± 0.013	2.977GlyAsn: 2.977 ± 0.017	2.278GlyPro: 2.278 ± 0.015	1.965GlyGln: 1.965 ± 0.014	3.965GlyArg: 3.965 ± 0.021	5.403GlySer: 5.403 ± 0.024	3.075GlyThr: 3.075 ± 0.017	4.447GlyVal: 4.447 ± 0.022	0.994GlyTrp: 0.994 ± 0.011	1.984GlyTyr: 1.984 ± 0.014	0.048GlyXaa: 0.048 ± 0.002
His	1.36HisAla: 1.36 ± 0.009	0.506HisCys: 0.506 ± 0.007	1.22HisAsp: 1.22 ± 0.011	1.344HisGlu: 1.344 ± 0.011	1.063HisPhe: 1.063 ± 0.01	1.611HisGly: 1.611 ± 0.011	0.864HisHis: 0.864 ± 0.01	1.155HisIle: 1.155 ± 0.009	1.225HisLys: 1.225 ± 0.009	2.266HisLeu: 2.266 ± 0.013	0.604HisMet: 0.604 ± 0.008	1.03HisAsn: 1.03 ± 0.009	1.385HisPro: 1.385 ± 0.011	1.102HisGln: 1.102 ± 0.011	1.3HisArg: 1.3 ± 0.011	1.735HisSer: 1.735 ± 0.013	0.942HisThr: 0.942 ± 0.009	1.596HisVal: 1.596 ± 0.013	0.345HisTrp: 0.345 ± 0.006	0.681HisTyr: 0.681 ± 0.007	0.017HisXaa: 0.017 ± 0.001
Ile	3.482IleAla: 3.482 ± 0.017	0.991IleCys: 0.991 ± 0.01	2.993IleAsp: 2.993 ± 0.016	3.482IleGlu: 3.482 ± 0.018	1.948IlePhe: 1.948 ± 0.013	3.207IleGly: 3.207 ± 0.017	1.288IleHis: 1.288 ± 0.011	2.509IleIle: 2.509 ± 0.017	2.997IleLys: 2.997 ± 0.015	4.573IleLeu: 4.573 ± 0.018	1.249IleMet: 1.249 ± 0.011	2.108IleAsn: 2.108 ± 0.013	2.991IlePro: 2.991 ± 0.017	1.936IleGln: 1.936 ± 0.014	2.497IleArg: 2.497 ± 0.015	4.242IleSer: 4.242 ± 0.02	2.539IleThr: 2.539 ± 0.014	3.462IleVal: 3.462 ± 0.019	0.774IleTrp: 0.774 ± 0.008	1.177IleTyr: 1.177 ± 0.01	0.036IleXaa: 0.036 ± 0.002
Lys	4.138LysAla: 4.138 ± 0.022	1.122LysCys: 1.122 ± 0.01	3.482LysAsp: 3.482 ± 0.018	5.407LysGlu: 5.407 ± 0.025	2.265LysPhe: 2.265 ± 0.015	3.942LysGly: 3.942 ± 0.02	1.333LysHis: 1.333 ± 0.012	3.261LysIle: 3.261 ± 0.018	5.574LysLys: 5.574 ± 0.03	5.944LysLeu: 5.944 ± 0.024	1.806LysMet: 1.806 ± 0.012	2.806LysAsn: 2.806 ± 0.017	2.705LysPro: 2.705 ± 0.017	2.434LysGln: 2.434 ± 0.015	3.706LysArg: 3.706 ± 0.019	4.596LysSer: 4.596 ± 0.021	3.073LysThr: 3.073 ± 0.018	4.361LysVal: 4.361 ± 0.022	0.96LysTrp: 0.96 ± 0.01	1.584LysTyr: 1.584 ± 0.013	0.05LysXaa: 0.05 ± 0.002
Leu	5.949LeuAla: 5.949 ± 0.023	1.705LeuCys: 1.705 ± 0.013	4.687LeuAsp: 4.687 ± 0.023	6.352LeuGlu: 6.352 ± 0.023	3.274LeuPhe: 3.274 ± 0.018	5.498LeuGly: 5.498 ± 0.025	2.288LeuHis: 2.288 ± 0.015	4.224LeuIle: 4.224 ± 0.021	6.449LeuLys: 6.449 ± 0.023	8.268LeuLeu: 8.268 ± 0.035	2.321LeuMet: 2.321 ± 0.017	3.721LeuAsn: 3.721 ± 0.02	4.716LeuPro: 4.716 ± 0.02	3.959LeuGln: 3.959 ± 0.023	5.217LeuArg: 5.217 ± 0.02	7.285LeuSer: 7.285 ± 0.028	4.244LeuThr: 4.244 ± 0.02	6.095LeuVal: 6.095 ± 0.023	1.238LeuTrp: 1.238 ± 0.01	1.927LeuTyr: 1.927 ± 0.014	0.067LeuXaa: 0.067 ± 0.002
Met	2.16MetAla: 2.16 ± 0.013	0.397MetCys: 0.397 ± 0.006	1.522MetAsp: 1.522 ± 0.01	2.242MetGlu: 2.242 ± 0.014	0.923MetPhe: 0.923 ± 0.009	1.562MetGly: 1.562 ± 0.012	0.614MetHis: 0.614 ± 0.008	1.304MetIle: 1.304 ± 0.012	2.021MetLys: 2.021 ± 0.013	2.329MetLeu: 2.329 ± 0.015	0.819MetMet: 0.819 ± 0.009	1.188MetAsn: 1.188 ± 0.011	1.101MetPro: 1.101 ± 0.011	1.022MetGln: 1.022 ± 0.009	1.406MetArg: 1.406 ± 0.011	1.961MetSer: 1.961 ± 0.012	1.27MetThr: 1.27 ± 0.012	1.885MetVal: 1.885 ± 0.013	0.317MetTrp: 0.317 ± 0.005	0.555MetTyr: 0.555 ± 0.006	0.016MetXaa: 0.016 ± 0.001
Asn	2.71AsnAla: 2.71 ± 0.016	0.866AsnCys: 0.866 ± 0.009	2.275AsnAsp: 2.275 ± 0.013	2.718AsnGlu: 2.718 ± 0.017	1.803AsnPhe: 1.803 ± 0.012	3.096AsnGly: 3.096 ± 0.018	1.124AsnHis: 1.124 ± 0.01	2.338AsnIle: 2.338 ± 0.015	2.683AsnLys: 2.683 ± 0.017	4.207AsnLeu: 4.207 ± 0.022	1.183AsnMet: 1.183 ± 0.011	2.483AsnAsn: 2.483 ± 0.021	2.301AsnPro: 2.301 ± 0.016	1.778AsnGln: 1.778 ± 0.014	2.031AsnArg: 2.031 ± 0.013	3.546AsnSer: 3.546 ± 0.018	2.159AsnThr: 2.159 ± 0.013	3.008AsnVal: 3.008 ± 0.017	0.639AsnTrp: 0.639 ± 0.008	1.133AsnTyr: 1.133 ± 0.01	0.039AsnXaa: 0.039 ± 0.002
Pro	3.025ProAla: 3.025 ± 0.02	0.648ProCys: 0.648 ± 0.008	2.622ProAsp: 2.622 ± 0.016	3.273ProGlu: 3.273 ± 0.017	1.871ProPhe: 1.871 ± 0.013	2.56ProGly: 2.56 ± 0.014	1.146ProHis: 1.146 ± 0.01	2.404ProIle: 2.404 ± 0.015	2.799ProLys: 2.799 ± 0.018	4.111ProLeu: 4.111 ± 0.02	1.112ProMet: 1.112 ± 0.01	2.146ProAsn: 2.146 ± 0.012	4.411ProPro: 4.411 ± 0.033	1.855ProGln: 1.855 ± 0.013	2.517ProArg: 2.517 ± 0.016	4.721ProSer: 4.721 ± 0.024	2.868ProThr: 2.868 ± 0.016	3.29ProVal: 3.29 ± 0.017	0.596ProTrp: 0.596 ± 0.007	1.296ProTyr: 1.296 ± 0.012	0.045ProXaa: 0.045 ± 0.002
Gln	2.407GlnAla: 2.407 ± 0.016	0.543GlnCys: 0.543 ± 0.007	1.685GlnAsp: 1.685 ± 0.014	2.662GlnGlu: 2.662 ± 0.015	1.384GlnPhe: 1.384 ± 0.012	2.377GlnGly: 2.377 ± 0.014	1.075GlnHis: 1.075 ± 0.01	1.924GlnIle: 1.924 ± 0.013	2.358GlnLys: 2.358 ± 0.013	3.409GlnLeu: 3.409 ± 0.018	1.11GlnMet: 1.11 ± 0.01	1.82GlnAsn: 1.82 ± 0.013	2.053GlnPro: 2.053 ± 0.016	2.279GlnGln: 2.279 ± 0.022	2.196GlnArg: 2.196 ± 0.014	2.58GlnSer: 2.58 ± 0.015	1.77GlnThr: 1.77 ± 0.013	2.418GlnVal: 2.418 ± 0.015	0.53GlnTrp: 0.53 ± 0.006	0.847GlnTyr: 0.847 ± 0.009	0.024GlnXaa: 0.024 ± 0.001
Arg	3.516ArgAla: 3.516 ± 0.021	1.102ArgCys: 1.102 ± 0.01	2.737ArgAsp: 2.737 ± 0.015	3.519ArgGlu: 3.519 ± 0.018	2.214ArgPhe: 2.214 ± 0.014	3.691ArgGly: 3.691 ± 0.019	1.207ArgHis: 1.207 ± 0.011	2.747ArgIle: 2.747 ± 0.016	3.84ArgLys: 3.84 ± 0.019	4.817ArgLeu: 4.817 ± 0.021	1.544ArgMet: 1.544 ± 0.012	2.451ArgAsn: 2.451 ± 0.016	2.377ArgPro: 2.377 ± 0.015	1.999ArgGln: 1.999 ± 0.014	4.3ArgArg: 4.3 ± 0.026	4.185ArgSer: 4.185 ± 0.02	2.493ArgThr: 2.493 ± 0.015	3.49ArgVal: 3.49 ± 0.019	0.86ArgTrp: 0.86 ± 0.009	1.337ArgTyr: 1.337 ± 0.01	0.037ArgXaa: 0.037 ± 0.002
Ser	4.719SerAla: 4.719 ± 0.022	1.44SerCys: 1.44 ± 0.012	4.438SerAsp: 4.438 ± 0.021	4.858SerGlu: 4.858 ± 0.022	3.252SerPhe: 3.252 ± 0.02	5.383SerGly: 5.383 ± 0.026	1.864SerHis: 1.864 ± 0.011	4.086SerIle: 4.086 ± 0.02	4.881SerLys: 4.881 ± 0.023	7.395SerLeu: 7.395 ± 0.029	2.014SerMet: 2.014 ± 0.012	3.637SerAsn: 3.637 ± 0.018	4.325SerPro: 4.325 ± 0.025	2.836SerGln: 2.836 ± 0.016	4.215SerArg: 4.215 ± 0.021	9.135SerSer: 9.135 ± 0.044	4.255SerThr: 4.255 ± 0.022	5.095SerVal: 5.095 ± 0.022	1.264SerTrp: 1.264 ± 0.012	1.919SerTyr: 1.919 ± 0.013	0.073SerXaa: 0.073 ± 0.003
Thr	3.407ThrAla: 3.407 ± 0.022	0.819ThrCys: 0.819 ± 0.009	2.456ThrAsp: 2.456 ± 0.015	2.968ThrGlu: 2.968 ± 0.019	1.832ThrPhe: 1.832 ± 0.013	3.009ThrGly: 3.009 ± 0.017	1.045ThrHis: 1.045 ± 0.009	2.69ThrIle: 2.69 ± 0.014	2.954ThrLys: 2.954 ± 0.015	4.406ThrLeu: 4.406 ± 0.021	1.363ThrMet: 1.363 ± 0.01	2.137ThrAsn: 2.137 ± 0.013	2.803ThrPro: 2.803 ± 0.016	1.683ThrGln: 1.683 ± 0.012	2.427ThrArg: 2.427 ± 0.016	4.386ThrSer: 4.386 ± 0.023	3.39ThrThr: 3.39 ± 0.024	3.393ThrVal: 3.393 ± 0.018	0.774ThrTrp: 0.774 ± 0.009	1.195ThrTyr: 1.195 ± 0.01	0.051ThrXaa: 0.051 ± 0.002
Val	5.155ValAla: 5.155 ± 0.021	1.274ValCys: 1.274 ± 0.012	4.289ValAsp: 4.289 ± 0.019	5.383ValGlu: 5.383 ± 0.02	2.655ValPhe: 2.655 ± 0.016	4.119ValGly: 4.119 ± 0.023	1.393ValHis: 1.393 ± 0.011	3.406ValIle: 3.406 ± 0.019	4.454ValLys: 4.454 ± 0.019	6.286ValLeu: 6.286 ± 0.025	1.794ValMet: 1.794 ± 0.011	2.818ValAsn: 2.818 ± 0.016	3.303ValPro: 3.303 ± 0.014	2.346ValGln: 2.346 ± 0.015	3.287ValArg: 3.287 ± 0.017	5.183ValSer: 5.183 ± 0.026	3.352ValThr: 3.352 ± 0.016	6.139ValVal: 6.139 ± 0.028	0.914ValTrp: 0.914 ± 0.01	1.636ValTyr: 1.636 ± 0.012	0.053ValXaa: 0.053 ± 0.002
Trp	0.7TrpAla: 0.7 ± 0.008	0.327TrpCys: 0.327 ± 0.006	0.723TrpAsp: 0.723 ± 0.008	0.977TrpGlu: 0.977 ± 0.009	0.56TrpPhe: 0.56 ± 0.007	0.935TrpGly: 0.935 ± 0.01	0.332TrpHis: 0.332 ± 0.005	0.765TrpIle: 0.765 ± 0.008	1.117TrpLys: 1.117 ± 0.01	1.339TrpLeu: 1.339 ± 0.009	0.392TrpMet: 0.392 ± 0.006	0.739TrpAsn: 0.739 ± 0.008	0.492TrpPro: 0.492 ± 0.006	0.458TrpGln: 0.458 ± 0.007	1.176TrpArg: 1.176 ± 0.01	1.064TrpSer: 1.064 ± 0.01	0.59TrpThr: 0.59 ± 0.008	0.942TrpVal: 0.942 ± 0.009	0.417TrpTrp: 0.417 ± 0.007	0.353TrpTyr: 0.353 ± 0.006	0.008TrpXaa: 0.008 ± 0.001
Tyr	1.663TyrAla: 1.663 ± 0.011	0.557TyrCys: 0.557 ± 0.007	1.464TyrAsp: 1.464 ± 0.012	1.488TyrGlu: 1.488 ± 0.012	1.125TyrPhe: 1.125 ± 0.009	1.871TyrGly: 1.871 ± 0.014	0.716TyrHis: 0.716 ± 0.008	1.12TyrIle: 1.12 ± 0.009	1.476TyrLys: 1.476 ± 0.011	2.273TyrLeu: 2.273 ± 0.015	0.645TyrMet: 0.645 ± 0.007	1.205TyrAsn: 1.205 ± 0.011	1.141TyrPro: 1.141 ± 0.01	0.918TyrGln: 0.918 ± 0.008	1.416TyrArg: 1.416 ± 0.01	1.814TyrSer: 1.814 ± 0.014	1.092TyrThr: 1.092 ± 0.01	1.595TyrVal: 1.595 ± 0.012	0.361TyrTrp: 0.361 ± 0.005	0.871TyrTyr: 0.871 ± 0.011	0.018TyrXaa: 0.018 ± 0.001
Xaa	0.043XaaAla: 0.043 ± 0.002	0.016XaaCys: 0.016 ± 0.001	0.045XaaAsp: 0.045 ± 0.002	0.054XaaGlu: 0.054 ± 0.002	0.039XaaPhe: 0.039 ± 0.002	0.054XaaGly: 0.054 ± 0.002	0.018XaaHis: 0.018 ± 0.001	0.04XaaIle: 0.04 ± 0.002	0.048XaaLys: 0.048 ± 0.002	0.065XaaLeu: 0.065 ± 0.002	0.019XaaMet: 0.019 ± 0.001	0.036XaaAsn: 0.036 ± 0.002	0.034XaaPro: 0.034 ± 0.002	0.026XaaGln: 0.026 ± 0.002	0.037XaaArg: 0.037 ± 0.002	0.059XaaSer: 0.059 ± 0.002	0.036XaaThr: 0.036 ± 0.002	0.058XaaVal: 0.058 ± 0.002	0.011XaaTrp: 0.011 ± 0.001	0.021XaaTyr: 0.021 ± 0.001	4.281XaaXaa: 4.281 ± 0.092

Statistics based on 116147 proteins (11926644 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---