Amino acid dipepetide frequency for Bacillus phage BSP9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.065AlaAla: 5.065 ± 0.629
0.293AlaCys: 0.293 ± 0.072
4.119AlaAsp: 4.119 ± 0.33
4.57AlaGlu: 4.57 ± 0.362
2.274AlaPhe: 2.274 ± 0.267
3.692AlaGly: 3.692 ± 0.519
1.103AlaHis: 1.103 ± 0.172
3.849AlaIle: 3.849 ± 0.318
4.344AlaLys: 4.344 ± 0.363
5.83AlaLeu: 5.83 ± 0.317
1.688AlaMet: 1.688 ± 0.2
2.476AlaAsn: 2.476 ± 0.308
2.161AlaPro: 2.161 ± 0.252
2.386AlaGln: 2.386 ± 0.299
2.544AlaArg: 2.544 ± 0.262
3.264AlaSer: 3.264 ± 0.354
3.872AlaThr: 3.872 ± 0.396
4.029AlaVal: 4.029 ± 0.354
0.585AlaTrp: 0.585 ± 0.106
2.769AlaTyr: 2.769 ± 0.254
0.0AlaXaa: 0.0 ± 0.0
Cys
0.405CysAla: 0.405 ± 0.096
0.135CysCys: 0.135 ± 0.055
0.563CysAsp: 0.563 ± 0.129
0.405CysGlu: 0.405 ± 0.089
0.428CysPhe: 0.428 ± 0.084
0.608CysGly: 0.608 ± 0.118
0.113CysHis: 0.113 ± 0.045
0.495CysIle: 0.495 ± 0.122
0.608CysLys: 0.608 ± 0.121
0.675CysLeu: 0.675 ± 0.122
0.18CysMet: 0.18 ± 0.063
0.36CysAsn: 0.36 ± 0.102
0.45CysPro: 0.45 ± 0.105
0.09CysGln: 0.09 ± 0.041
0.338CysArg: 0.338 ± 0.089
0.698CysSer: 0.698 ± 0.129
0.248CysThr: 0.248 ± 0.088
0.473CysVal: 0.473 ± 0.109
0.09CysTrp: 0.09 ± 0.041
0.27CysTyr: 0.27 ± 0.064
0.0CysXaa: 0.0 ± 0.0
Asp
3.647AspAla: 3.647 ± 0.342
0.338AspCys: 0.338 ± 0.103
3.399AspAsp: 3.399 ± 0.352
4.615AspGlu: 4.615 ± 0.331
3.196AspPhe: 3.196 ± 0.302
4.232AspGly: 4.232 ± 0.45
0.945AspHis: 0.945 ± 0.134
4.93AspIle: 4.93 ± 0.337
4.592AspLys: 4.592 ± 0.374
5.853AspLeu: 5.853 ± 0.425
2.206AspMet: 2.206 ± 0.224
3.174AspAsn: 3.174 ± 0.27
2.116AspPro: 2.116 ± 0.202
2.003AspGln: 2.003 ± 0.216
2.791AspArg: 2.791 ± 0.223
3.917AspSer: 3.917 ± 0.336
4.344AspThr: 4.344 ± 0.336
5.222AspVal: 5.222 ± 0.416
0.765AspTrp: 0.765 ± 0.136
3.557AspTyr: 3.557 ± 0.303
0.0AspXaa: 0.0 ± 0.0
Glu
5.29GluAla: 5.29 ± 0.31
0.608GluCys: 0.608 ± 0.147
5.808GluAsp: 5.808 ± 0.442
9.229GluGlu: 9.229 ± 0.749
2.814GluPhe: 2.814 ± 0.269
5.245GluGly: 5.245 ± 0.331
1.688GluHis: 1.688 ± 0.225
5.02GluIle: 5.02 ± 0.4
5.965GluLys: 5.965 ± 0.392
7.001GluLeu: 7.001 ± 0.449
2.679GluMet: 2.679 ± 0.262
3.512GluAsn: 3.512 ± 0.225
2.071GluPro: 2.071 ± 0.276
2.881GluGln: 2.881 ± 0.283
4.052GluArg: 4.052 ± 0.36
3.782GluSer: 3.782 ± 0.253
3.534GluThr: 3.534 ± 0.316
6.055GluVal: 6.055 ± 0.394
1.08GluTrp: 1.08 ± 0.171
3.579GluTyr: 3.579 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
1.666PheAla: 1.666 ± 0.192
0.518PheCys: 0.518 ± 0.111
2.521PheAsp: 2.521 ± 0.237
2.994PheGlu: 2.994 ± 0.301
1.463PhePhe: 1.463 ± 0.214
2.341PheGly: 2.341 ± 0.249
0.743PheHis: 0.743 ± 0.124
2.971PheIle: 2.971 ± 0.239
2.971PheLys: 2.971 ± 0.242
3.084PheLeu: 3.084 ± 0.245
0.833PheMet: 0.833 ± 0.132
2.544PheAsn: 2.544 ± 0.269
1.328PhePro: 1.328 ± 0.153
1.216PheGln: 1.216 ± 0.185
1.486PheArg: 1.486 ± 0.149
3.129PheSer: 3.129 ± 0.275
2.724PheThr: 2.724 ± 0.245
2.454PheVal: 2.454 ± 0.257
0.405PheTrp: 0.405 ± 0.103
1.688PheTyr: 1.688 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
3.602GlyAla: 3.602 ± 0.55
0.833GlyCys: 0.833 ± 0.142
4.052GlyAsp: 4.052 ± 0.38
4.75GlyGlu: 4.75 ± 0.363
2.409GlyPhe: 2.409 ± 0.211
4.84GlyGly: 4.84 ± 0.677
1.171GlyHis: 1.171 ± 0.15
4.277GlyIle: 4.277 ± 0.387
4.975GlyLys: 4.975 ± 0.386
4.885GlyLeu: 4.885 ± 0.379
1.531GlyMet: 1.531 ± 0.206
3.692GlyAsn: 3.692 ± 0.285
0.518GlyPro: 0.518 ± 0.099
2.116GlyGln: 2.116 ± 0.267
2.791GlyArg: 2.791 ± 0.213
4.209GlySer: 4.209 ± 0.414
3.939GlyThr: 3.939 ± 0.38
4.592GlyVal: 4.592 ± 0.315
0.833GlyTrp: 0.833 ± 0.137
3.264GlyTyr: 3.264 ± 0.245
0.0GlyXaa: 0.0 ± 0.0
His
1.193HisAla: 1.193 ± 0.16
0.203HisCys: 0.203 ± 0.06
0.9HisAsp: 0.9 ± 0.145
0.878HisGlu: 0.878 ± 0.122
0.72HisPhe: 0.72 ± 0.127
1.103HisGly: 1.103 ± 0.154
0.495HisHis: 0.495 ± 0.133
1.531HisIle: 1.531 ± 0.197
1.238HisLys: 1.238 ± 0.191
1.778HisLeu: 1.778 ± 0.239
0.608HisMet: 0.608 ± 0.089
0.99HisAsn: 0.99 ± 0.147
0.608HisPro: 0.608 ± 0.119
0.585HisGln: 0.585 ± 0.106
0.99HisArg: 0.99 ± 0.14
1.328HisSer: 1.328 ± 0.202
0.855HisThr: 0.855 ± 0.137
1.643HisVal: 1.643 ± 0.16
0.27HisTrp: 0.27 ± 0.08
1.035HisTyr: 1.035 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
3.962IleAla: 3.962 ± 0.33
0.428IleCys: 0.428 ± 0.087
4.48IleAsp: 4.48 ± 0.394
4.862IleGlu: 4.862 ± 0.36
1.688IlePhe: 1.688 ± 0.149
3.332IleGly: 3.332 ± 0.34
1.238IleHis: 1.238 ± 0.131
3.084IleIle: 3.084 ± 0.285
4.885IleLys: 4.885 ± 0.314
4.299IleLeu: 4.299 ± 0.387
1.598IleMet: 1.598 ± 0.162
3.332IleAsn: 3.332 ± 0.304
2.386IlePro: 2.386 ± 0.231
2.476IleGln: 2.476 ± 0.207
2.791IleArg: 2.791 ± 0.244
4.457IleSer: 4.457 ± 0.357
4.232IleThr: 4.232 ± 0.42
4.074IleVal: 4.074 ± 0.307
0.428IleTrp: 0.428 ± 0.106
2.229IleTyr: 2.229 ± 0.241
0.0IleXaa: 0.0 ± 0.0
Lys
5.312LysAla: 5.312 ± 0.39
0.495LysCys: 0.495 ± 0.098
4.772LysAsp: 4.772 ± 0.322
7.744LysGlu: 7.744 ± 0.576
2.791LysPhe: 2.791 ± 0.281
5.155LysGly: 5.155 ± 0.414
1.711LysHis: 1.711 ± 0.184
3.332LysIle: 3.332 ± 0.268
6.821LysLys: 6.821 ± 0.499
5.853LysLeu: 5.853 ± 0.363
1.868LysMet: 1.868 ± 0.21
3.557LysAsn: 3.557 ± 0.3
2.431LysPro: 2.431 ± 0.231
3.084LysGln: 3.084 ± 0.294
3.624LysArg: 3.624 ± 0.299
4.48LysSer: 4.48 ± 0.492
4.142LysThr: 4.142 ± 0.315
4.93LysVal: 4.93 ± 0.362
0.923LysTrp: 0.923 ± 0.136
3.309LysTyr: 3.309 ± 0.312
0.0LysXaa: 0.0 ± 0.0
Leu
4.907LeuAla: 4.907 ± 0.366
0.63LeuCys: 0.63 ± 0.15
6.663LeuAsp: 6.663 ± 0.428
7.046LeuGlu: 7.046 ± 0.443
3.061LeuPhe: 3.061 ± 0.287
4.885LeuGly: 4.885 ± 0.328
1.373LeuHis: 1.373 ± 0.178
4.412LeuIle: 4.412 ± 0.324
6.798LeuLys: 6.798 ± 0.392
6.348LeuLeu: 6.348 ± 0.399
1.913LeuMet: 1.913 ± 0.215
4.502LeuAsn: 4.502 ± 0.329
3.061LeuPro: 3.061 ± 0.263
2.904LeuGln: 2.904 ± 0.268
3.714LeuArg: 3.714 ± 0.307
5.943LeuSer: 5.943 ± 0.346
5.583LeuThr: 5.583 ± 0.376
5.2LeuVal: 5.2 ± 0.359
0.788LeuTrp: 0.788 ± 0.117
3.332LeuTyr: 3.332 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
1.643MetAla: 1.643 ± 0.183
0.135MetCys: 0.135 ± 0.056
1.846MetAsp: 1.846 ± 0.22
1.958MetGlu: 1.958 ± 0.206
1.171MetPhe: 1.171 ± 0.173
1.508MetGly: 1.508 ± 0.201
0.383MetHis: 0.383 ± 0.095
1.283MetIle: 1.283 ± 0.171
2.364MetLys: 2.364 ± 0.246
2.048MetLeu: 2.048 ± 0.214
0.45MetMet: 0.45 ± 0.099
1.553MetAsn: 1.553 ± 0.181
0.72MetPro: 0.72 ± 0.134
0.99MetGln: 0.99 ± 0.136
1.216MetArg: 1.216 ± 0.189
2.319MetSer: 2.319 ± 0.263
1.823MetThr: 1.823 ± 0.218
1.08MetVal: 1.08 ± 0.139
0.158MetTrp: 0.158 ± 0.056
1.193MetTyr: 1.193 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
2.949AsnAla: 2.949 ± 0.29
0.495AsnCys: 0.495 ± 0.111
2.679AsnAsp: 2.679 ± 0.28
3.219AsnGlu: 3.219 ± 0.228
1.778AsnPhe: 1.778 ± 0.206
4.029AsnGly: 4.029 ± 0.352
1.126AsnHis: 1.126 ± 0.172
3.669AsnIle: 3.669 ± 0.306
4.142AsnLys: 4.142 ± 0.34
3.939AsnLeu: 3.939 ± 0.294
1.306AsnMet: 1.306 ± 0.189
3.174AsnAsn: 3.174 ± 0.296
2.589AsnPro: 2.589 ± 0.282
1.666AsnGln: 1.666 ± 0.214
2.499AsnArg: 2.499 ± 0.251
3.151AsnSer: 3.151 ± 0.333
3.196AsnThr: 3.196 ± 0.27
3.512AsnVal: 3.512 ± 0.244
0.518AsnTrp: 0.518 ± 0.104
2.071AsnTyr: 2.071 ± 0.208
0.0AsnXaa: 0.0 ± 0.0
Pro
2.454ProAla: 2.454 ± 0.412
0.18ProCys: 0.18 ± 0.064
2.274ProAsp: 2.274 ± 0.221
3.151ProGlu: 3.151 ± 0.353
1.306ProPhe: 1.306 ± 0.2
0.968ProGly: 0.968 ± 0.161
0.72ProHis: 0.72 ± 0.136
1.913ProIle: 1.913 ± 0.205
2.251ProLys: 2.251 ± 0.23
2.521ProLeu: 2.521 ± 0.262
0.518ProMet: 0.518 ± 0.129
1.891ProAsn: 1.891 ± 0.209
0.9ProPro: 0.9 ± 0.16
1.306ProGln: 1.306 ± 0.187
1.148ProArg: 1.148 ± 0.156
1.958ProSer: 1.958 ± 0.252
2.679ProThr: 2.679 ± 0.243
2.138ProVal: 2.138 ± 0.211
0.203ProTrp: 0.203 ± 0.062
1.643ProTyr: 1.643 ± 0.206
0.0ProXaa: 0.0 ± 0.0
Gln
2.206GlnAla: 2.206 ± 0.263
0.225GlnCys: 0.225 ± 0.068
1.981GlnAsp: 1.981 ± 0.207
3.714GlnGlu: 3.714 ± 0.306
1.328GlnPhe: 1.328 ± 0.175
1.733GlnGly: 1.733 ± 0.195
0.608GlnHis: 0.608 ± 0.116
2.026GlnIle: 2.026 ± 0.214
2.679GlnLys: 2.679 ± 0.213
3.287GlnLeu: 3.287 ± 0.24
1.351GlnMet: 1.351 ± 0.182
2.026GlnAsn: 2.026 ± 0.221
1.126GlnPro: 1.126 ± 0.237
1.981GlnGln: 1.981 ± 0.268
2.071GlnArg: 2.071 ± 0.209
2.206GlnSer: 2.206 ± 0.235
1.598GlnThr: 1.598 ± 0.203
2.836GlnVal: 2.836 ± 0.222
0.293GlnTrp: 0.293 ± 0.082
1.328GlnTyr: 1.328 ± 0.165
0.0GlnXaa: 0.0 ± 0.0
Arg
2.206ArgAla: 2.206 ± 0.261
0.36ArgCys: 0.36 ± 0.109
2.611ArgAsp: 2.611 ± 0.27
3.939ArgGlu: 3.939 ± 0.299
2.161ArgPhe: 2.161 ± 0.239
3.264ArgGly: 3.264 ± 0.272
0.81ArgHis: 0.81 ± 0.143
2.949ArgIle: 2.949 ± 0.261
3.917ArgLys: 3.917 ± 0.364
4.344ArgLeu: 4.344 ± 0.309
1.373ArgMet: 1.373 ± 0.196
2.048ArgAsn: 2.048 ± 0.244
1.08ArgPro: 1.08 ± 0.173
1.733ArgGln: 1.733 ± 0.222
2.138ArgArg: 2.138 ± 0.244
2.319ArgSer: 2.319 ± 0.249
2.769ArgThr: 2.769 ± 0.286
3.467ArgVal: 3.467 ± 0.331
0.495ArgTrp: 0.495 ± 0.095
2.138ArgTyr: 2.138 ± 0.251
0.0ArgXaa: 0.0 ± 0.0
Ser
3.084SerAla: 3.084 ± 0.295
0.383SerCys: 0.383 ± 0.111
3.579SerAsp: 3.579 ± 0.287
4.299SerGlu: 4.299 ± 0.325
3.061SerPhe: 3.061 ± 0.251
4.187SerGly: 4.187 ± 0.348
1.058SerHis: 1.058 ± 0.161
3.894SerIle: 3.894 ± 0.366
5.357SerLys: 5.357 ± 0.413
5.493SerLeu: 5.493 ± 0.285
1.778SerMet: 1.778 ± 0.214
3.309SerAsn: 3.309 ± 0.355
1.801SerPro: 1.801 ± 0.202
2.161SerGln: 2.161 ± 0.21
2.791SerArg: 2.791 ± 0.291
4.84SerSer: 4.84 ± 0.325
4.344SerThr: 4.344 ± 0.388
4.637SerVal: 4.637 ± 0.36
0.81SerTrp: 0.81 ± 0.137
2.701SerTyr: 2.701 ± 0.24
0.0SerXaa: 0.0 ± 0.0
Thr
4.074ThrAla: 4.074 ± 0.465
0.293ThrCys: 0.293 ± 0.081
4.367ThrAsp: 4.367 ± 0.398
4.682ThrGlu: 4.682 ± 0.381
2.454ThrPhe: 2.454 ± 0.236
4.682ThrGly: 4.682 ± 0.442
1.148ThrHis: 1.148 ± 0.174
3.624ThrIle: 3.624 ± 0.332
3.737ThrLys: 3.737 ± 0.265
5.47ThrLeu: 5.47 ± 0.356
1.193ThrMet: 1.193 ± 0.166
3.039ThrAsn: 3.039 ± 0.299
2.634ThrPro: 2.634 ± 0.24
2.319ThrGln: 2.319 ± 0.193
2.859ThrArg: 2.859 ± 0.288
3.467ThrSer: 3.467 ± 0.291
4.187ThrThr: 4.187 ± 0.423
5.02ThrVal: 5.02 ± 0.307
0.54ThrTrp: 0.54 ± 0.095
2.724ThrTyr: 2.724 ± 0.263
0.0ThrXaa: 0.0 ± 0.0
Val
4.029ValAla: 4.029 ± 0.274
0.585ValCys: 0.585 ± 0.154
5.155ValAsp: 5.155 ± 0.329
5.425ValGlu: 5.425 ± 0.4
2.859ValPhe: 2.859 ± 0.249
3.917ValGly: 3.917 ± 0.269
1.418ValHis: 1.418 ± 0.21
3.624ValIle: 3.624 ± 0.29
4.885ValLys: 4.885 ± 0.351
5.087ValLeu: 5.087 ± 0.39
1.666ValMet: 1.666 ± 0.196
3.602ValAsn: 3.602 ± 0.304
2.881ValPro: 2.881 ± 0.255
2.611ValGln: 2.611 ± 0.22
3.399ValArg: 3.399 ± 0.283
4.975ValSer: 4.975 ± 0.358
4.84ValThr: 4.84 ± 0.39
4.502ValVal: 4.502 ± 0.331
0.653ValTrp: 0.653 ± 0.141
3.151ValTyr: 3.151 ± 0.297
0.0ValXaa: 0.0 ± 0.0
Trp
0.54TrpAla: 0.54 ± 0.119
0.09TrpCys: 0.09 ± 0.048
0.923TrpAsp: 0.923 ± 0.129
0.945TrpGlu: 0.945 ± 0.146
0.54TrpPhe: 0.54 ± 0.114
0.653TrpGly: 0.653 ± 0.139
0.225TrpHis: 0.225 ± 0.066
0.518TrpIle: 0.518 ± 0.099
0.81TrpLys: 0.81 ± 0.127
0.675TrpLeu: 0.675 ± 0.136
0.203TrpMet: 0.203 ± 0.069
0.45TrpAsn: 0.45 ± 0.1
0.0TrpPro: 0.0 ± 0.0
0.36TrpGln: 0.36 ± 0.089
0.428TrpArg: 0.428 ± 0.102
0.563TrpSer: 0.563 ± 0.117
0.675TrpThr: 0.675 ± 0.121
0.9TrpVal: 0.9 ± 0.154
0.203TrpTrp: 0.203 ± 0.089
0.72TrpTyr: 0.72 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.701TyrAla: 2.701 ± 0.209
0.405TyrCys: 0.405 ± 0.093
3.151TyrAsp: 3.151 ± 0.29
3.467TyrGlu: 3.467 ± 0.26
1.576TyrPhe: 1.576 ± 0.174
2.904TyrGly: 2.904 ± 0.265
0.878TyrHis: 0.878 ± 0.147
2.859TyrIle: 2.859 ± 0.239
2.994TyrLys: 2.994 ± 0.284
4.592TyrLeu: 4.592 ± 0.335
0.878TyrMet: 0.878 ± 0.128
2.476TyrAsn: 2.476 ± 0.26
1.261TyrPro: 1.261 ± 0.177
1.733TyrGln: 1.733 ± 0.199
2.454TyrArg: 2.454 ± 0.228
2.566TyrSer: 2.566 ± 0.28
2.926TyrThr: 2.926 ± 0.276
2.499TyrVal: 2.499 ± 0.215
0.383TyrTrp: 0.383 ± 0.078
1.891TyrTyr: 1.891 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 228 proteins (44425 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski