Cacao swollen shoot Ghana J virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus; unclassified Badnavirus

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4U938|A0A2H4U938_9VIRU Reverse transcriptase OS=Cacao swollen shoot Ghana J virus OX=2056880 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 6.4SIPDD6 pKa = 3.6GNQTRR11 pKa = 11.84RR12 pKa = 11.84ACEE15 pKa = 3.58IPATQLNGASYY26 pKa = 9.74PFSLAYY32 pKa = 10.16DD33 pKa = 3.63GLLQQRR39 pKa = 11.84QDD41 pKa = 3.83VITHH45 pKa = 5.94GTLSLSKK52 pKa = 9.88DD53 pKa = 3.41TAIQSQLYY61 pKa = 9.84KK62 pKa = 10.14IEE64 pKa = 3.95EE65 pKa = 3.99QAARR69 pKa = 11.84EE70 pKa = 4.01ALKK73 pKa = 10.63ALHH76 pKa = 6.93DD77 pKa = 4.07FQGILHH83 pKa = 6.92HH84 pKa = 6.56KK85 pKa = 9.52RR86 pKa = 11.84AYY88 pKa = 9.57LHH90 pKa = 6.96SSATRR95 pKa = 11.84DD96 pKa = 2.8NWAGDD101 pKa = 3.53RR102 pKa = 11.84LPGVRR107 pKa = 11.84QASEE111 pKa = 4.01NLDD114 pKa = 3.77LYY116 pKa = 10.93SAAIASIIEE125 pKa = 4.25QVVQPP130 pKa = 4.42

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4U936|A0A2H4U936_9VIRU ORFY protein OS=Cacao swollen shoot Ghana J virus OX=2056880 PE=4 SV=1
MM1 pKa = 6.86SQEE4 pKa = 3.43EE5 pKa = 4.12RR6 pKa = 11.84KK7 pKa = 10.06YY8 pKa = 11.25GKK10 pKa = 9.43NLAMAMVDD18 pKa = 3.47SFDD21 pKa = 4.45TILSLKK27 pKa = 9.6ATMIPLILSQLDD39 pKa = 3.45GVMSRR44 pKa = 11.84KK45 pKa = 9.83KK46 pKa = 10.57FLILHH51 pKa = 6.34AMKK54 pKa = 10.7IMMTVMIVMMMKK66 pKa = 9.97TGIHH70 pKa = 6.35HH71 pKa = 6.94FLILINQMQKK81 pKa = 10.26KK82 pKa = 9.37KK83 pKa = 10.83RR84 pKa = 11.84SIPTNKK90 pKa = 9.57IPTNRR95 pKa = 11.84MMRR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84KK101 pKa = 8.44MKK103 pKa = 10.82NMLCC107 pKa = 3.8

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2392

107

1868

478.4

54.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.727 ± 1.38

1.547 ± 0.588

4.933 ± 0.639

8.11 ± 0.883

3.261 ± 0.983

4.849 ± 0.394

2.885 ± 0.471

6.773 ± 0.492

6.94 ± 1.365

9.49 ± 1.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 1.297

4.264 ± 0.376

4.222 ± 0.622

6.396 ± 0.648

5.518 ± 0.382

6.773 ± 0.425

6.187 ± 0.579

4.975 ± 0.608

1.254 ± 0.332

3.177 ± 0.579
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski