Termite gut associated microvirus 1
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3IST8|A0A3G3IST8_9VIRU DNA pilot protein OS=Termite gut associated microvirus 1 OX=2486188 PE=4 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.46 QSVSVDD9 pKa = 3.16 DD10 pKa = 3.89 VVIYY14 pKa = 9.38 SHH16 pKa = 6.69 YY17 pKa = 9.68 YY18 pKa = 7.31 PRR20 pKa = 11.84 RR21 pKa = 11.84 VPGITFTEE29 pKa = 4.17 PTMAQQHH36 pKa = 5.53 FRR38 pKa = 11.84 DD39 pKa = 3.7 EE40 pKa = 4.36 CDD42 pKa = 2.78 INTIMSRR49 pKa = 11.84 YY50 pKa = 8.43 EE51 pKa = 3.76 RR52 pKa = 11.84 TGVLVDD58 pKa = 3.95 PLQQRR63 pKa = 11.84 TAKK66 pKa = 10.52 PMFDD70 pKa = 4.39 DD71 pKa = 5.17 FSVIPDD77 pKa = 3.45 YY78 pKa = 11.48 QEE80 pKa = 3.72 AQNAIIQARR89 pKa = 11.84 YY90 pKa = 9.91 LFDD93 pKa = 4.27 NLPASIRR100 pKa = 11.84 KK101 pKa = 9.16 RR102 pKa = 11.84 FNNDD106 pKa = 2.89 PAQLLDD112 pKa = 5.15 FMADD116 pKa = 3.19 DD117 pKa = 4.44 SNFEE121 pKa = 3.91 EE122 pKa = 4.99 AVALGIVAKK131 pKa = 10.19 PLEE134 pKa = 4.61 TPPKK138 pKa = 10.4 SEE140 pKa = 5.0 DD141 pKa = 3.38 KK142 pKa = 10.47 PSEE145 pKa = 4.08 PEE147 pKa = 4.1 VVNKK151 pKa = 10.55 DD152 pKa = 3.41 VTPP155 pKa = 3.96
Molecular weight: 17.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.631
IPC2_protein 4.596
IPC_protein 4.52
Toseland 4.342
ProMoST 4.647
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.393
Rodwell 4.355
Grimsley 4.253
Solomon 4.482
Lehninger 4.444
Nozaki 4.596
DTASelect 4.813
Thurlkill 4.368
EMBOSS 4.406
Sillero 4.635
Patrickios 3.999
IPC_peptide 4.482
IPC2_peptide 4.622
IPC2.peptide.svr19 4.598
Protein with the highest isoelectric point:
>tr|A0A3G3IST8|A0A3G3IST8_9VIRU DNA pilot protein OS=Termite gut associated microvirus 1 OX=2486188 PE=4 SV=1
MM1 pKa = 6.88 QVASARR7 pKa = 11.84 EE8 pKa = 3.61 QMAFQEE14 pKa = 4.01 RR15 pKa = 11.84 MFRR18 pKa = 11.84 NRR20 pKa = 11.84 HH21 pKa = 4.18 TYY23 pKa = 8.73 EE24 pKa = 3.87 VEE26 pKa = 3.73 DD27 pKa = 4.28 LKK29 pKa = 11.19 RR30 pKa = 11.84 AGLNPILSAHH40 pKa = 6.45 SGGSTPSGAMVGGMEE55 pKa = 4.35 NVGEE59 pKa = 4.2 SAVRR63 pKa = 11.84 GFSAAQQASIAKK75 pKa = 9.69 DD76 pKa = 3.21 QLALNKK82 pKa = 10.28 SKK84 pKa = 11.08 LEE86 pKa = 3.92 SEE88 pKa = 4.25 IAVNSATAQRR98 pKa = 11.84 QQSEE102 pKa = 4.32 SALIKK107 pKa = 10.36 SQVDD111 pKa = 3.06 SGYY114 pKa = 9.97 YY115 pKa = 9.31 PSLVGLNNASAQEE128 pKa = 3.87 RR129 pKa = 11.84 AAQIPLIEE137 pKa = 4.35 SSVRR141 pKa = 11.84 LNGQRR146 pKa = 11.84 ILEE149 pKa = 4.19 SDD151 pKa = 3.35 ARR153 pKa = 11.84 VQQLRR158 pKa = 11.84 KK159 pKa = 10.02 DD160 pKa = 3.08 ISLIEE165 pKa = 3.81 EE166 pKa = 4.76 RR167 pKa = 11.84 IRR169 pKa = 11.84 TEE171 pKa = 3.45 RR172 pKa = 11.84 SLRR175 pKa = 11.84 ALNAANTQLTYY186 pKa = 11.08 LRR188 pKa = 11.84 GALTEE193 pKa = 4.2 EE194 pKa = 4.49 EE195 pKa = 4.59 TRR197 pKa = 11.84 TQQNITSLQGYY208 pKa = 8.48 LAEE211 pKa = 4.38 AQRR214 pKa = 11.84 LGIPEE219 pKa = 4.25 KK220 pKa = 10.72 LIGYY224 pKa = 9.07 IKK226 pKa = 10.49 YY227 pKa = 10.64 SNPRR231 pKa = 11.84 LQSLLSQGLTKK242 pKa = 10.47 QEE244 pKa = 4.71 LYY246 pKa = 10.3 PFAIGTGQSLFNSAVEE262 pKa = 4.23 KK263 pKa = 10.86 ARR265 pKa = 11.84 SNLPNRR271 pKa = 11.84 VNRR274 pKa = 11.84 SHH276 pKa = 7.02 KK277 pKa = 10.31 RR278 pKa = 3.27
Molecular weight: 30.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.15
IPC2_protein 9.165
IPC_protein 9.311
Toseland 9.809
ProMoST 9.677
Dawson 10.058
Bjellqvist 9.75
Wikipedia 10.262
Rodwell 10.292
Grimsley 10.145
Solomon 10.101
Lehninger 10.058
Nozaki 9.765
DTASelect 9.75
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.97
Patrickios 7.644
IPC_peptide 10.101
IPC2_peptide 8.302
IPC2.peptide.svr19 8.277
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1384
91
567
276.8
31.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.587 ± 0.93
1.301 ± 0.612
6.431 ± 1.152
5.13 ± 1.047
4.408 ± 0.889
6.72 ± 1.017
2.24 ± 0.567
5.347 ± 0.185
3.251 ± 0.711
7.442 ± 0.825
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.806 ± 0.299
4.697 ± 0.451
5.708 ± 0.952
5.202 ± 0.963
7.514 ± 1.031
7.298 ± 0.897
5.058 ± 0.522
6.792 ± 0.789
1.156 ± 0.463
4.913 ± 0.636
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here