Mycobacterium phage Charm

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JCP5|A0A482JCP5_9CAUD Uncharacterized protein OS=Mycobacterium phage Charm OX=2517942 GN=1 PE=4 SV=1
MM1 pKa = 7.75ARR3 pKa = 11.84ATYY6 pKa = 11.25VNMIDD11 pKa = 4.57RR12 pKa = 11.84YY13 pKa = 9.84QLFAGEE19 pKa = 4.46PMLDD23 pKa = 3.24TSEE26 pKa = 4.16GVLIIQYY33 pKa = 10.54HH34 pKa = 7.11DD35 pKa = 3.42GTSRR39 pKa = 11.84TINWQHH45 pKa = 6.45VIDD48 pKa = 4.98FYY50 pKa = 11.86YY51 pKa = 8.22MTDD54 pKa = 3.43DD55 pKa = 4.05EE56 pKa = 5.01YY57 pKa = 11.73ADD59 pKa = 3.76FLQQIEE65 pKa = 4.42EE66 pKa = 4.19EE67 pKa = 4.15QEE69 pKa = 4.14EE70 pKa = 4.33EE71 pKa = 4.74DD72 pKa = 4.44EE73 pKa = 4.36EE74 pKa = 4.43

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J9F3|A0A482J9F3_9CAUD Uncharacterized protein OS=Mycobacterium phage Charm OX=2517942 GN=39 PE=4 SV=1
MM1 pKa = 7.35SWADD5 pKa = 2.94SDD7 pKa = 4.52RR8 pKa = 11.84RR9 pKa = 11.84QNLPPDD15 pKa = 3.24WEE17 pKa = 4.2EE18 pKa = 3.49RR19 pKa = 11.84RR20 pKa = 11.84LAILVDD26 pKa = 4.12ANWVCEE32 pKa = 3.67IRR34 pKa = 11.84MAGCTRR40 pKa = 11.84VATDD44 pKa = 2.8VDD46 pKa = 4.34HH47 pKa = 7.45IKK49 pKa = 10.61RR50 pKa = 11.84GNDD53 pKa = 2.83HH54 pKa = 5.42SHH56 pKa = 6.57RR57 pKa = 11.84NLRR60 pKa = 11.84AACGWCHH67 pKa = 6.24DD68 pKa = 4.39RR69 pKa = 11.84KK70 pKa = 10.78SSAEE74 pKa = 3.67GVARR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 3.88LKK83 pKa = 10.28ARR85 pKa = 11.84RR86 pKa = 11.84KK87 pKa = 9.71RR88 pKa = 11.84PPEE91 pKa = 3.62RR92 pKa = 11.84HH93 pKa = 5.71PGSRR97 pKa = 3.46

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16524

30

1031

179.6

19.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.173 ± 0.438

0.902 ± 0.13

6.348 ± 0.219

6.53 ± 0.297

3.486 ± 0.15

8.388 ± 0.449

1.846 ± 0.172

5.059 ± 0.166

4.726 ± 0.244

8.2 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.153

3.649 ± 0.185

5.35 ± 0.297

3.51 ± 0.185

6.112 ± 0.309

5.368 ± 0.17

6.203 ± 0.314

6.978 ± 0.24

1.997 ± 0.144

2.748 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski