Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355)

Taxonomy: cellular organisms; Bacteria; Fusobacteria; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Fusobacterium; Fusobacterium nucleatum; Fusobacterium nucleatum subsp. nucleatum

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2046 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8RGG8|RS9_FUSNN 30S ribosomal protein S9 OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=rpsI PE=3 SV=1
MM1 pKa = 7.39GMYY4 pKa = 10.43GSYY7 pKa = 10.49ISLEE11 pKa = 4.08KK12 pKa = 10.74KK13 pKa = 10.08EE14 pKa = 4.47LEE16 pKa = 4.35KK17 pKa = 11.12LLNGNKK23 pKa = 10.25DD24 pKa = 3.08FDD26 pKa = 5.43DD27 pKa = 4.27IEE29 pKa = 4.59SVEE32 pKa = 4.25TLDD35 pKa = 5.82IDD37 pKa = 4.94KK38 pKa = 10.53SWQAIQYY45 pKa = 10.15LLGGDD50 pKa = 3.04ICEE53 pKa = 4.25IEE55 pKa = 4.62GPLGYY60 pKa = 9.9VVPMLVEE67 pKa = 3.85NAIDD71 pKa = 3.88CDD73 pKa = 4.05SEE75 pKa = 4.76FGVFYY80 pKa = 10.01ITTEE84 pKa = 4.17QIKK87 pKa = 9.58EE88 pKa = 4.06AYY90 pKa = 8.7DD91 pKa = 3.41ALFPLTKK98 pKa = 10.15EE99 pKa = 4.34DD100 pKa = 5.0LLEE103 pKa = 4.44KK104 pKa = 11.03YY105 pKa = 10.2NFSEE109 pKa = 4.06MLEE112 pKa = 4.31DD113 pKa = 3.2EE114 pKa = 4.82VYY116 pKa = 10.73PIVEE120 pKa = 4.91DD121 pKa = 4.24DD122 pKa = 4.08DD123 pKa = 4.13EE124 pKa = 6.5KK125 pKa = 11.63EE126 pKa = 4.05FFDD129 pKa = 4.83YY130 pKa = 10.43IYY132 pKa = 10.87SYY134 pKa = 11.22LLEE137 pKa = 4.73IKK139 pKa = 10.14EE140 pKa = 4.21FYY142 pKa = 10.14KK143 pKa = 11.01KK144 pKa = 10.61NIEE147 pKa = 4.06KK148 pKa = 10.29EE149 pKa = 4.01LAILFYY155 pKa = 10.65ISS157 pKa = 3.13

Molecular weight: 18.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8R5X8|TRUB_FUSNN tRNA pseudouridine synthase B OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=truB PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNQRR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84KK13 pKa = 8.19KK14 pKa = 10.11DD15 pKa = 2.98HH16 pKa = 6.09GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.26NGRR28 pKa = 11.84KK29 pKa = 7.3VLKK32 pKa = 9.7RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84VRR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.66LSAA44 pKa = 4.0

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2046

0

2046

641087

20

3165

313.3

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.462 ± 0.064

0.773 ± 0.021

5.402 ± 0.043

7.869 ± 0.075

4.909 ± 0.06

6.348 ± 0.097

1.18 ± 0.021

9.962 ± 0.081

10.111 ± 0.059

9.339 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.029

6.388 ± 0.07

2.552 ± 0.03

2.161 ± 0.022

3.194 ± 0.033

5.978 ± 0.048

4.853 ± 0.061

6.089 ± 0.041

0.622 ± 0.016

4.422 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski