Pigeonpox virus
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 222 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068EER1|A0A068EER1_9POXV Major core protein 4a precursor OS=Pigeonpox virus OX=10264 GN=fep_178 PE=3 SV=1
MM1 pKa = 7.76 IIYY4 pKa = 10.24 IITLSLLFIKK14 pKa = 10.68 VITSSKK20 pKa = 10.48 EE21 pKa = 3.87 PVKK24 pKa = 10.83 NPQDD28 pKa = 3.13 ILHH31 pKa = 6.73 IIEE34 pKa = 4.42 HH35 pKa = 6.23 NKK37 pKa = 9.44 TGVTACSLYY46 pKa = 11.02 CFDD49 pKa = 4.71 SSKK52 pKa = 11.34 GLDD55 pKa = 3.42 QPKK58 pKa = 8.87 TFILPGKK65 pKa = 9.66 YY66 pKa = 9.99 SNNSIKK72 pKa = 10.87 LEE74 pKa = 4.03 VAIDD78 pKa = 3.67 IYY80 pKa = 11.5 KK81 pKa = 10.48 KK82 pKa = 10.6 GSKK85 pKa = 9.63 SDD87 pKa = 3.72 YY88 pKa = 10.55 SHH90 pKa = 7.7 PCQAFQFCVSGNFSGKK106 pKa = 10.17 RR107 pKa = 11.84 FDD109 pKa = 3.75 HH110 pKa = 6.1 YY111 pKa = 11.25 LYY113 pKa = 10.52 GYY115 pKa = 7.99 TITGFIDD122 pKa = 3.19 IASSYY127 pKa = 9.19 YY128 pKa = 10.61 SGMSISTITLMPLQEE143 pKa = 4.48 GSLNHH148 pKa = 7.2 DD149 pKa = 3.9 SEE151 pKa = 5.0 EE152 pKa = 4.57 DD153 pKa = 3.65 CTTPPISTITQYY165 pKa = 11.05 QRR167 pKa = 11.84 IPEE170 pKa = 4.48 PIIKK174 pKa = 9.55 EE175 pKa = 3.91 GCKK178 pKa = 9.75 PVILQRR184 pKa = 11.84 YY185 pKa = 8.53 GEE187 pKa = 4.48 SDD189 pKa = 4.02 DD190 pKa = 4.39 PTCIMYY196 pKa = 9.51 WDD198 pKa = 4.04 NTWDD202 pKa = 3.61 NYY204 pKa = 10.87 CDD206 pKa = 3.22 VGFFNSQQRR215 pKa = 11.84 DD216 pKa = 3.51 HH217 pKa = 7.59 DD218 pKa = 4.5 PLVLPLNNYY227 pKa = 10.06 LGISDD232 pKa = 4.97 AFQDD236 pKa = 3.93 FQSYY240 pKa = 8.5 YY241 pKa = 10.68 CKK243 pKa = 10.68 SLDD246 pKa = 3.71 LNQSYY251 pKa = 9.43 SVCISIGEE259 pKa = 4.37 TPTTVTYY266 pKa = 10.51 HH267 pKa = 6.15 SYY269 pKa = 11.69 EE270 pKa = 4.32 NITVNEE276 pKa = 4.14 LLTRR280 pKa = 11.84 IMILYY285 pKa = 10.37 GEE287 pKa = 4.56 EE288 pKa = 4.29 KK289 pKa = 9.97 VHH291 pKa = 6.77 KK292 pKa = 10.53 LPFRR296 pKa = 11.84 NITIMAHH303 pKa = 5.56 AQIQSLPLINSTCDD317 pKa = 3.31 PNKK320 pKa = 10.27 FDD322 pKa = 6.77 DD323 pKa = 6.23 DD324 pKa = 6.01 DD325 pKa = 7.53 DD326 pKa = 7.6 DD327 pKa = 7.65 DD328 pKa = 7.59 DD329 pKa = 7.65 DD330 pKa = 7.65 DD331 pKa = 7.65 DD332 pKa = 7.65 DD333 pKa = 7.65 DD334 pKa = 7.65 DD335 pKa = 7.65 DD336 pKa = 7.65 DD337 pKa = 7.62 DD338 pKa = 7.59 DD339 pKa = 7.71 DD340 pKa = 7.58 DD341 pKa = 6.68 DD342 pKa = 6.84 DD343 pKa = 4.87 EE344 pKa = 5.58 YY345 pKa = 12.15 NLYY348 pKa = 9.63 TEE350 pKa = 4.42 STPSKK355 pKa = 10.01 VTTKK359 pKa = 10.03 PKK361 pKa = 9.53 KK362 pKa = 9.14 TITDD366 pKa = 3.6 EE367 pKa = 3.89 YY368 pKa = 11.46 DD369 pKa = 3.4 SIFNSFDD376 pKa = 3.27 NFDD379 pKa = 3.66 LEE381 pKa = 4.61 KK382 pKa = 10.78 RR383 pKa = 3.75
Molecular weight: 43.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.974
IPC2_protein 4.19
IPC_protein 4.215
Toseland 3.999
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.355
Wikipedia 4.151
Rodwell 4.05
Grimsley 3.91
Solomon 4.202
Lehninger 4.164
Nozaki 4.317
DTASelect 4.596
Thurlkill 4.05
EMBOSS 4.151
Sillero 4.342
Patrickios 0.998
IPC_peptide 4.202
IPC2_peptide 4.317
IPC2.peptide.svr19 4.206
Protein with the highest isoelectric point:
>tr|A0A068EE70|A0A068EE70_9POXV DNA polymerase OS=Pigeonpox virus OX=10264 GN=fep_096 PE=3 SV=1
MM1 pKa = 7.54 EE2 pKa = 5.37 IARR5 pKa = 11.84 EE6 pKa = 3.92 TLITIGLTILVVVLVITGFSLVLRR30 pKa = 11.84 LIPGVYY36 pKa = 9.71 SAASRR41 pKa = 11.84 SSFTAGKK48 pKa = 9.17 VLRR51 pKa = 11.84 FMEE54 pKa = 4.3 IFSTVMFIPGIIILYY69 pKa = 9.17 AAYY72 pKa = 9.46 IRR74 pKa = 11.84 KK75 pKa = 7.84 TKK77 pKa = 9.57 MKK79 pKa = 10.73 NNN81 pKa = 3.69
Molecular weight: 8.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.285
IPC2_protein 9.911
IPC_protein 10.511
Toseland 10.526
ProMoST 10.204
Dawson 10.672
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 11.038
Grimsley 10.745
Solomon 10.745
Lehninger 10.716
Nozaki 10.482
DTASelect 10.365
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.584
Patrickios 10.833
IPC_peptide 10.745
IPC2_peptide 9.121
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
222
0
222
73067
34
1937
329.1
38.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.32 ± 0.105
2.203 ± 0.08
6.438 ± 0.119
5.674 ± 0.099
4.293 ± 0.123
3.861 ± 0.116
2.005 ± 0.058
10.274 ± 0.199
8.38 ± 0.14
9.193 ± 0.166
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.505 ± 0.057
7.344 ± 0.149
3.099 ± 0.106
1.99 ± 0.062
4.01 ± 0.083
7.768 ± 0.179
5.677 ± 0.118
5.637 ± 0.106
0.63 ± 0.039
5.698 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here