Turkey adenovirus 1
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0YC53|E0YC53_9ADEN ORF1B OS=Turkey adenovirus 1 OX=878329 PE=4 SV=1
DDD2 pKa = 6.08 ISVGDDD8 pKa = 3.92 PDDD11 pKa = 3.19 AVRR14 pKa = 11.84 LSVEEE19 pKa = 4.1 AGEEE23 pKa = 4.17 EE24 pKa = 4.19 EE25 pKa = 4.43 GGGSCNCFCEEE36 pKa = 4.56 DDD38 pKa = 3.67 GKKK41 pKa = 8.83 TSCSLCTCPVSCSIS
Molecular weight: 5.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.925
IPC2_protein 3.821
IPC_protein 3.63
Toseland 3.465
ProMoST 3.923
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.401
Solomon 3.567
Lehninger 3.528
Nozaki 3.783
DTASelect 3.897
Thurlkill 3.554
EMBOSS 3.592
Sillero 3.757
Patrickios 0.006
IPC_peptide 3.567
IPC2_peptide 3.719
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|E0YC66|E0YC66_9ADEN PX OS=Turkey adenovirus 1 OX=878329 PE=4 SV=1
MM1 pKa = 7.74 SILISPSDD9 pKa = 3.55 NRR11 pKa = 11.84 GWGAAMRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 CRR22 pKa = 11.84 SSMRR26 pKa = 11.84 GVGLRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 TTTVRR39 pKa = 11.84 PLTLRR44 pKa = 11.84 SLLGLGARR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 GRR58 pKa = 11.84 ASSRR62 pKa = 11.84 VSSRR66 pKa = 11.84 LVVVRR71 pKa = 11.84 TSRR74 pKa = 11.84 TSRR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 3.32
Molecular weight: 9.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.501
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.12
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.183
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
1
37
13075
56
1320
353.4
39.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.69 ± 0.581
1.736 ± 0.236
5.683 ± 0.419
5.996 ± 0.418
3.816 ± 0.259
7.87 ± 0.862
2.241 ± 0.223
2.264 ± 0.199
2.6 ± 0.2
9.293 ± 0.387
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.996 ± 0.22
3.189 ± 0.451
7.526 ± 0.418
3.526 ± 0.349
9.231 ± 0.575
6.05 ± 0.326
5.423 ± 0.403
7.197 ± 0.374
1.293 ± 0.14
3.38 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here