Aquimarina amphilecti

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Aquimarina

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4646 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7Q8U0|A0A1H7Q8U0_9FLAO CubicO group peptidase beta-lactamase class C family OS=Aquimarina amphilecti OX=1038014 GN=SAMN04487910_2452 PE=4 SV=1
MM1 pKa = 7.26NKK3 pKa = 9.86IITFLIFVCTISITAQNSYY22 pKa = 10.21RR23 pKa = 11.84INYY26 pKa = 7.53QAVVRR31 pKa = 11.84DD32 pKa = 3.57AGNIVVGDD40 pKa = 3.99TNVGVQINILQTSASGTSVYY60 pKa = 10.82SEE62 pKa = 4.12NQSVTTNINGLASLVIGNAAGFDD85 pKa = 4.09SIDD88 pKa = 3.5WSAGPYY94 pKa = 9.83FIRR97 pKa = 11.84IEE99 pKa = 3.97IDD101 pKa = 3.15PAGGTSYY108 pKa = 10.23TLSTVNQLLSVPYY121 pKa = 9.55ALHH124 pKa = 6.69SATADD129 pKa = 3.3VAATANYY136 pKa = 10.41NSLTNLPTIITPAQAAKK153 pKa = 10.04IDD155 pKa = 4.62FITVTSAVNIDD166 pKa = 3.56QLQTDD171 pKa = 3.95VTANNAKK178 pKa = 8.65VTFPGFGTVPGTALEE193 pKa = 4.29GDD195 pKa = 3.57NVIWTKK201 pKa = 11.56SMTDD205 pKa = 3.5DD206 pKa = 3.11IFYY209 pKa = 10.65NAGNVGIGVDD219 pKa = 3.9EE220 pKa = 4.54SSSFSGSKK228 pKa = 9.99LHH230 pKa = 6.55VGGPILFDD238 pKa = 3.29GTPAATVPGSLYY250 pKa = 10.69YY251 pKa = 10.89DD252 pKa = 3.47NTGSGSFHH260 pKa = 7.03YY261 pKa = 10.17IDD263 pKa = 3.77NTNTDD268 pKa = 3.13VTINTGAVTYY278 pKa = 10.7AGGLWSIEE286 pKa = 3.98NGDD289 pKa = 3.78AVISNDD295 pKa = 3.35VFIQNSLGVGQDD307 pKa = 2.43ISTGVDD313 pKa = 3.8FGFNTLLLAEE323 pKa = 4.41NNLRR327 pKa = 11.84ILFDD331 pKa = 4.83DD332 pKa = 4.26SDD334 pKa = 5.25DD335 pKa = 4.05ISGTLPANDD344 pKa = 3.39WQIEE348 pKa = 4.19INEE351 pKa = 4.33TANGGTSHH359 pKa = 7.2FAIVDD364 pKa = 3.59VTGATTPFKK373 pKa = 10.43ILAGAAEE380 pKa = 4.36HH381 pKa = 6.43SLFVATNGNVGLGTNNPTNKK401 pKa = 10.14LEE403 pKa = 4.09VVGAIKK409 pKa = 10.59ADD411 pKa = 3.68SFIGDD416 pKa = 3.53GSGITGIATGTGGVTNAGDD435 pKa = 3.47TTIAADD441 pKa = 3.72NDD443 pKa = 3.68ADD445 pKa = 4.31TVGEE449 pKa = 4.52IAFQTQNITRR459 pKa = 11.84MTVSNTGFIGIGTTTPSVALDD480 pKa = 3.66VIGDD484 pKa = 3.82AKK486 pKa = 10.29VDD488 pKa = 3.5NMTLNGNASVQSIVITPNVEE508 pKa = 3.99TSATTFPTTYY518 pKa = 10.95DD519 pKa = 3.39VTDD522 pKa = 3.53KK523 pKa = 11.6SFVTLDD529 pKa = 3.36GQAASSIQAFSGGITGQSIIVTALTSTITLEE560 pKa = 4.38HH561 pKa = 6.31NAGTAAQNFQLAGNANIILTANSSATFVYY590 pKa = 10.55DD591 pKa = 3.18GTNWYY596 pKa = 10.15CVGLNNN602 pKa = 5.33

Molecular weight: 62.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7QFH8|A0A1H7QFH8_9FLAO Glutamate 5-kinase OS=Aquimarina amphilecti OX=1038014 GN=proB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.66LSVSSEE47 pKa = 3.9LRR49 pKa = 11.84HH50 pKa = 5.43KK51 pKa = 10.29HH52 pKa = 5.04

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4646

0

4646

1587142

36

4991

341.6

38.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.603 ± 0.037

0.751 ± 0.011

5.799 ± 0.033

6.552 ± 0.032

5.238 ± 0.032

6.407 ± 0.039

1.652 ± 0.015

8.522 ± 0.041

7.825 ± 0.057

9.075 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.012 ± 0.015

6.485 ± 0.033

3.26 ± 0.023

3.297 ± 0.019

3.381 ± 0.022

6.905 ± 0.029

5.971 ± 0.041

5.994 ± 0.032

1.085 ± 0.014

4.185 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski