Amino acid dipepetide frequency for Eubacteriaceae bacterium CHKCI005

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.985AlaAla: 7.985 ± 0.145	1.233AlaCys: 1.233 ± 0.038	4.695AlaAsp: 4.695 ± 0.095	4.711AlaGlu: 4.711 ± 0.098	3.119AlaPhe: 3.119 ± 0.073	6.283AlaGly: 6.283 ± 0.115	1.4AlaHis: 1.4 ± 0.047	5.088AlaIle: 5.088 ± 0.097	4.35AlaLys: 4.35 ± 0.089	7.917AlaLeu: 7.917 ± 0.115	2.372AlaMet: 2.372 ± 0.071	2.42AlaAsn: 2.42 ± 0.06	2.646AlaPro: 2.646 ± 0.061	3.374AlaGln: 3.374 ± 0.084	3.621AlaArg: 3.621 ± 0.074	5.003AlaSer: 5.003 ± 0.102	3.71AlaThr: 3.71 ± 0.106	6.998AlaVal: 6.998 ± 0.109	0.691AlaTrp: 0.691 ± 0.034	2.441AlaTyr: 2.441 ± 0.065	0.0AlaXaa: 0.0 ± 0.0
Cys	1.165CysAla: 1.165 ± 0.046	0.363CysCys: 0.363 ± 0.027	0.894CysAsp: 0.894 ± 0.036	0.825CysGlu: 0.825 ± 0.037	0.585CysPhe: 0.585 ± 0.032	1.563CysGly: 1.563 ± 0.06	0.317CysHis: 0.317 ± 0.022	0.994CysIle: 0.994 ± 0.046	0.745CysLys: 0.745 ± 0.034	1.259CysLeu: 1.259 ± 0.047	0.445CysMet: 0.445 ± 0.021	0.506CysAsn: 0.506 ± 0.03	0.804CysPro: 0.804 ± 0.037	0.503CysGln: 0.503 ± 0.028	0.946CysArg: 0.946 ± 0.037	0.976CysSer: 0.976 ± 0.038	0.84CysThr: 0.84 ± 0.034	1.026CysVal: 1.026 ± 0.045	0.117CysTrp: 0.117 ± 0.015	0.483CysTyr: 0.483 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.82AspAla: 4.82 ± 0.086	0.864AspCys: 0.864 ± 0.037	3.242AspAsp: 3.242 ± 0.086	4.055AspGlu: 4.055 ± 0.078	2.504AspPhe: 2.504 ± 0.062	5.112AspGly: 5.112 ± 0.1	1.093AspHis: 1.093 ± 0.04	4.287AspIle: 4.287 ± 0.08	2.994AspLys: 2.994 ± 0.059	5.236AspLeu: 5.236 ± 0.083	1.673AspMet: 1.673 ± 0.043	2.16AspAsn: 2.16 ± 0.064	2.607AspPro: 2.607 ± 0.068	2.399AspGln: 2.399 ± 0.06	2.813AspArg: 2.813 ± 0.066	3.389AspSer: 3.389 ± 0.084	3.311AspThr: 3.311 ± 0.077	4.298AspVal: 4.298 ± 0.086	0.702AspTrp: 0.702 ± 0.035	2.345AspTyr: 2.345 ± 0.066	0.0AspXaa: 0.0 ± 0.0
Glu	5.639GluAla: 5.639 ± 0.104	0.669GluCys: 0.669 ± 0.03	4.133GluAsp: 4.133 ± 0.077	5.886GluGlu: 5.886 ± 0.103	1.959GluPhe: 1.959 ± 0.05	4.771GluGly: 4.771 ± 0.08	1.322GluHis: 1.322 ± 0.045	4.191GluIle: 4.191 ± 0.085	4.677GluLys: 4.677 ± 0.086	5.734GluLeu: 5.734 ± 0.107	2.183GluMet: 2.183 ± 0.057	3.272GluAsn: 3.272 ± 0.077	2.083GluPro: 2.083 ± 0.063	3.408GluGln: 3.408 ± 0.09	3.321GluArg: 3.321 ± 0.08	3.307GluSer: 3.307 ± 0.082	3.472GluThr: 3.472 ± 0.078	4.34GluVal: 4.34 ± 0.089	0.54GluTrp: 0.54 ± 0.031	2.232GluTyr: 2.232 ± 0.072	0.0GluXaa: 0.0 ± 0.0
Phe	2.887PheAla: 2.887 ± 0.068	0.715PheCys: 0.715 ± 0.034	2.79PheAsp: 2.79 ± 0.064	2.471PheGlu: 2.471 ± 0.063	1.63PhePhe: 1.63 ± 0.063	3.045PheGly: 3.045 ± 0.073	0.901PheHis: 0.901 ± 0.034	2.151PheIle: 2.151 ± 0.064	1.375PheLys: 1.375 ± 0.046	3.774PheLeu: 3.774 ± 0.101	0.851PheMet: 0.851 ± 0.032	1.357PheAsn: 1.357 ± 0.045	1.416PhePro: 1.416 ± 0.042	1.434PheGln: 1.434 ± 0.042	1.544PheArg: 1.544 ± 0.043	2.854PheSer: 2.854 ± 0.073	2.31PheThr: 2.31 ± 0.059	2.769PheVal: 2.769 ± 0.068	0.416PheTrp: 0.416 ± 0.026	1.512PheTyr: 1.512 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	5.48GlyAla: 5.48 ± 0.123	1.346GlyCys: 1.346 ± 0.05	4.24GlyAsp: 4.24 ± 0.081	4.898GlyGlu: 4.898 ± 0.097	2.911GlyPhe: 2.911 ± 0.062	5.543GlyGly: 5.543 ± 0.119	1.304GlyHis: 1.304 ± 0.044	5.697GlyIle: 5.697 ± 0.093	4.818GlyLys: 4.818 ± 0.083	6.583GlyLeu: 6.583 ± 0.104	2.379GlyMet: 2.379 ± 0.056	2.863GlyAsn: 2.863 ± 0.077	2.026GlyPro: 2.026 ± 0.145	3.115GlyGln: 3.115 ± 0.069	3.798GlyArg: 3.798 ± 0.088	4.735GlySer: 4.735 ± 0.088	4.223GlyThr: 4.223 ± 0.093	5.533GlyVal: 5.533 ± 0.094	0.84GlyTrp: 0.84 ± 0.035	2.84GlyTyr: 2.84 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	1.288HisAla: 1.288 ± 0.043	0.398HisCys: 0.398 ± 0.024	0.876HisAsp: 0.876 ± 0.036	1.001HisGlu: 1.001 ± 0.037	0.807HisPhe: 0.807 ± 0.029	1.381HisGly: 1.381 ± 0.051	0.403HisHis: 0.403 ± 0.024	1.341HisIle: 1.341 ± 0.044	0.864HisLys: 0.864 ± 0.03	1.808HisLeu: 1.808 ± 0.052	0.491HisMet: 0.491 ± 0.027	0.709HisAsn: 0.709 ± 0.029	1.228HisPro: 1.228 ± 0.049	0.754HisGln: 0.754 ± 0.035	1.078HisArg: 1.078 ± 0.038	1.108HisSer: 1.108 ± 0.038	1.028HisThr: 1.028 ± 0.045	1.214HisVal: 1.214 ± 0.041	0.236HisTrp: 0.236 ± 0.018	0.812HisTyr: 0.812 ± 0.043	0.0HisXaa: 0.0 ± 0.0
Ile	5.32IleAla: 5.32 ± 0.092	1.035IleCys: 1.035 ± 0.04	3.787IleAsp: 3.787 ± 0.077	3.685IleGlu: 3.685 ± 0.076	2.3IlePhe: 2.3 ± 0.067	4.564IleGly: 4.564 ± 0.091	1.215IleHis: 1.215 ± 0.047	3.472IleIle: 3.472 ± 0.083	2.919IleLys: 2.919 ± 0.064	6.626IleLeu: 6.626 ± 0.114	1.463IleMet: 1.463 ± 0.052	2.257IleAsn: 2.257 ± 0.053	3.26IlePro: 3.26 ± 0.068	2.837IleGln: 2.837 ± 0.059	3.689IleArg: 3.689 ± 0.085	4.28IleSer: 4.28 ± 0.083	3.803IleThr: 3.803 ± 0.072	4.748IleVal: 4.748 ± 0.084	0.551IleTrp: 0.551 ± 0.031	2.073IleTyr: 2.073 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	4.789LysAla: 4.789 ± 0.084	0.577LysCys: 0.577 ± 0.031	3.25LysAsp: 3.25 ± 0.065	4.303LysGlu: 4.303 ± 0.086	1.352LysPhe: 1.352 ± 0.044	3.878LysGly: 3.878 ± 0.076	0.891LysHis: 0.891 ± 0.04	3.218LysIle: 3.218 ± 0.079	3.938LysLys: 3.938 ± 0.096	4.436LysLeu: 4.436 ± 0.081	1.717LysMet: 1.717 ± 0.055	2.117LysAsn: 2.117 ± 0.053	2.0LysPro: 2.0 ± 0.058	2.409LysGln: 2.409 ± 0.073	3.123LysArg: 3.123 ± 0.07	3.05LysSer: 3.05 ± 0.071	3.102LysThr: 3.102 ± 0.069	3.725LysVal: 3.725 ± 0.084	0.597LysTrp: 0.597 ± 0.036	1.726LysTyr: 1.726 ± 0.047	0.0LysXaa: 0.0 ± 0.0
Leu	7.401LeuAla: 7.401 ± 0.117	1.798LeuCys: 1.798 ± 0.053	5.791LeuAsp: 5.791 ± 0.095	6.227LeuGlu: 6.227 ± 0.121	3.862LeuPhe: 3.862 ± 0.1	6.512LeuGly: 6.512 ± 0.103	1.837LeuHis: 1.837 ± 0.056	5.319LeuIle: 5.319 ± 0.111	5.053LeuLys: 5.053 ± 0.098	9.221LeuLeu: 9.221 ± 0.157	2.542LeuMet: 2.542 ± 0.062	3.274LeuAsn: 3.274 ± 0.073	4.315LeuPro: 4.315 ± 0.088	3.247LeuGln: 3.247 ± 0.071	4.515LeuArg: 4.515 ± 0.091	7.224LeuSer: 7.224 ± 0.112	5.421LeuThr: 5.421 ± 0.088	5.867LeuVal: 5.867 ± 0.099	0.826LeuTrp: 0.826 ± 0.037	3.162LeuTyr: 3.162 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.6MetAla: 2.6 ± 0.066	0.328MetCys: 0.328 ± 0.023	1.898MetAsp: 1.898 ± 0.054	2.197MetGlu: 2.197 ± 0.059	0.847MetPhe: 0.847 ± 0.032	2.194MetGly: 2.194 ± 0.061	0.409MetHis: 0.409 ± 0.026	1.649MetIle: 1.649 ± 0.053	1.862MetLys: 1.862 ± 0.045	2.741MetLeu: 2.741 ± 0.063	0.85MetMet: 0.85 ± 0.038	1.071MetAsn: 1.071 ± 0.038	1.284MetPro: 1.284 ± 0.041	1.042MetGln: 1.042 ± 0.036	1.449MetArg: 1.449 ± 0.051	1.754MetSer: 1.754 ± 0.054	1.672MetThr: 1.672 ± 0.044	1.979MetVal: 1.979 ± 0.058	0.209MetTrp: 0.209 ± 0.018	0.648MetTyr: 0.648 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.938AsnAla: 2.938 ± 0.067	0.605AsnCys: 0.605 ± 0.031	1.948AsnAsp: 1.948 ± 0.07	2.094AsnGlu: 2.094 ± 0.068	1.336AsnPhe: 1.336 ± 0.047	3.243AsnGly: 3.243 ± 0.09	0.704AsnHis: 0.704 ± 0.034	2.595AsnIle: 2.595 ± 0.06	1.653AsnLys: 1.653 ± 0.051	3.527AsnLeu: 3.527 ± 0.068	1.001AsnMet: 1.001 ± 0.039	1.475AsnAsn: 1.475 ± 0.065	2.051AsnPro: 2.051 ± 0.051	1.502AsnGln: 1.502 ± 0.049	2.005AsnArg: 2.005 ± 0.059	1.998AsnSer: 1.998 ± 0.067	2.079AsnThr: 2.079 ± 0.062	2.557AsnVal: 2.557 ± 0.068	0.469AsnTrp: 0.469 ± 0.031	1.393AsnTyr: 1.393 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	3.191ProAla: 3.191 ± 0.084	0.541ProCys: 0.541 ± 0.034	3.034ProAsp: 3.034 ± 0.069	3.46ProGlu: 3.46 ± 0.077	1.609ProPhe: 1.609 ± 0.042	2.82ProGly: 2.82 ± 0.069	0.791ProHis: 0.791 ± 0.034	2.328ProIle: 2.328 ± 0.06	1.966ProLys: 1.966 ± 0.057	3.315ProLeu: 3.315 ± 0.085	1.11ProMet: 1.11 ± 0.039	1.361ProAsn: 1.361 ± 0.043	1.409ProPro: 1.409 ± 0.059	1.667ProGln: 1.667 ± 0.072	1.398ProArg: 1.398 ± 0.05	2.849ProSer: 2.849 ± 0.059	2.101ProThr: 2.101 ± 0.089	3.522ProVal: 3.522 ± 0.078	0.395ProTrp: 0.395 ± 0.026	1.542ProTyr: 1.542 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	3.083GlnAla: 3.083 ± 0.066	0.624GlnCys: 0.624 ± 0.032	2.1GlnAsp: 2.1 ± 0.056	2.977GlnGlu: 2.977 ± 0.072	1.519GlnPhe: 1.519 ± 0.046	2.724GlnGly: 2.724 ± 0.097	0.67GlnHis: 0.67 ± 0.031	2.395GlnIle: 2.395 ± 0.063	2.826GlnLys: 2.826 ± 0.065	3.72GlnLeu: 3.72 ± 0.076	1.396GlnMet: 1.396 ± 0.044	1.951GlnAsn: 1.951 ± 0.05	1.489GlnPro: 1.489 ± 0.046	1.756GlnGln: 1.756 ± 0.054	1.975GlnArg: 1.975 ± 0.066	2.575GlnSer: 2.575 ± 0.071	1.954GlnThr: 1.954 ± 0.053	2.966GlnVal: 2.966 ± 0.075	0.567GlnTrp: 0.567 ± 0.028	1.756GlnTyr: 1.756 ± 0.057	0.0GlnXaa: 0.0 ± 0.0
Arg	3.286ArgAla: 3.286 ± 0.082	0.731ArgCys: 0.731 ± 0.039	2.62ArgAsp: 2.62 ± 0.066	3.71ArgGlu: 3.71 ± 0.087	2.108ArgPhe: 2.108 ± 0.062	2.901ArgGly: 2.901 ± 0.067	0.983ArgHis: 0.983 ± 0.034	3.639ArgIle: 3.639 ± 0.073	2.881ArgLys: 2.881 ± 0.075	5.02ArgLeu: 5.02 ± 0.104	1.709ArgMet: 1.709 ± 0.054	1.773ArgAsn: 1.773 ± 0.053	1.929ArgPro: 1.929 ± 0.059	2.328ArgGln: 2.328 ± 0.071	3.189ArgArg: 3.189 ± 0.093	2.706ArgSer: 2.706 ± 0.069	2.389ArgThr: 2.389 ± 0.06	3.239ArgVal: 3.239 ± 0.076	0.526ArgTrp: 0.526 ± 0.029	1.979ArgTyr: 1.979 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	4.945SerAla: 4.945 ± 0.1	0.919SerCys: 0.919 ± 0.044	3.778SerAsp: 3.778 ± 0.083	3.554SerGlu: 3.554 ± 0.077	2.895SerPhe: 2.895 ± 0.062	5.234SerGly: 5.234 ± 0.094	1.231SerHis: 1.231 ± 0.04	4.482SerIle: 4.482 ± 0.083	2.976SerLys: 2.976 ± 0.069	5.98SerLeu: 5.98 ± 0.107	1.877SerMet: 1.877 ± 0.051	2.208SerAsn: 2.208 ± 0.072	2.58SerPro: 2.58 ± 0.063	2.667SerGln: 2.667 ± 0.067	3.112SerArg: 3.112 ± 0.077	5.12SerSer: 5.12 ± 0.145	3.272SerThr: 3.272 ± 0.087	4.578SerVal: 4.578 ± 0.088	0.667SerTrp: 0.667 ± 0.038	2.233SerTyr: 2.233 ± 0.071	0.0SerXaa: 0.0 ± 0.0
Thr	4.884ThrAla: 4.884 ± 0.097	0.674ThrCys: 0.674 ± 0.031	3.115ThrAsp: 3.115 ± 0.076	2.948ThrGlu: 2.948 ± 0.068	2.149ThrPhe: 2.149 ± 0.061	5.055ThrGly: 5.055 ± 0.173	1.036ThrHis: 1.036 ± 0.04	3.845ThrIle: 3.845 ± 0.082	2.364ThrLys: 2.364 ± 0.064	5.261ThrLeu: 5.261 ± 0.08	1.45ThrMet: 1.45 ± 0.043	1.767ThrAsn: 1.767 ± 0.054	2.662ThrPro: 2.662 ± 0.063	1.879ThrGln: 1.879 ± 0.052	2.133ThrArg: 2.133 ± 0.053	3.421ThrSer: 3.421 ± 0.088	2.895ThrThr: 2.895 ± 0.077	4.937ThrVal: 4.937 ± 0.109	0.545ThrTrp: 0.545 ± 0.035	1.856ThrTyr: 1.856 ± 0.07	0.0ThrXaa: 0.0 ± 0.0
Val	5.347ValAla: 5.347 ± 0.097	1.22ValCys: 1.22 ± 0.043	4.585ValAsp: 4.585 ± 0.089	5.042ValGlu: 5.042 ± 0.086	2.738ValPhe: 2.738 ± 0.061	4.871ValGly: 4.871 ± 0.095	1.275ValHis: 1.275 ± 0.046	4.449ValIle: 4.449 ± 0.079	3.934ValLys: 3.934 ± 0.081	7.124ValLeu: 7.124 ± 0.131	2.023ValMet: 2.023 ± 0.06	2.721ValAsn: 2.721 ± 0.073	2.854ValPro: 2.854 ± 0.058	2.609ValGln: 2.609 ± 0.055	3.401ValArg: 3.401 ± 0.074	5.18ValSer: 5.18 ± 0.095	4.675ValThr: 4.675 ± 0.091	5.366ValVal: 5.366 ± 0.106	0.659ValTrp: 0.659 ± 0.033	2.415ValTyr: 2.415 ± 0.067	0.0ValXaa: 0.0 ± 0.0
Trp	0.637TrpAla: 0.637 ± 0.031	0.181TrpCys: 0.181 ± 0.015	0.669TrpAsp: 0.669 ± 0.033	0.74TrpGlu: 0.74 ± 0.032	0.398TrpPhe: 0.398 ± 0.024	0.719TrpGly: 0.719 ± 0.034	0.193TrpHis: 0.193 ± 0.018	0.68TrpIle: 0.68 ± 0.031	0.573TrpLys: 0.573 ± 0.03	0.918TrpLeu: 0.918 ± 0.038	0.395TrpMet: 0.395 ± 0.023	0.491TrpAsn: 0.491 ± 0.033	0.271TrpPro: 0.271 ± 0.024	0.47TrpGln: 0.47 ± 0.029	0.47TrpArg: 0.47 ± 0.028	0.562TrpSer: 0.562 ± 0.034	0.534TrpThr: 0.534 ± 0.037	0.61TrpVal: 0.61 ± 0.033	0.128TrpTrp: 0.128 ± 0.015	0.346TrpTyr: 0.346 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.371TyrAla: 2.371 ± 0.064	0.535TyrCys: 0.535 ± 0.03	2.46TyrAsp: 2.46 ± 0.068	2.354TyrGlu: 2.354 ± 0.058	1.573TyrPhe: 1.573 ± 0.053	2.759TyrGly: 2.759 ± 0.066	0.845TyrHis: 0.845 ± 0.034	2.064TyrIle: 2.064 ± 0.063	1.428TyrLys: 1.428 ± 0.05	3.218TyrLeu: 3.218 ± 0.077	0.751TyrMet: 0.751 ± 0.032	1.421TyrAsn: 1.421 ± 0.044	1.499TyrPro: 1.499 ± 0.052	1.626TyrGln: 1.626 ± 0.045	2.066TyrArg: 2.066 ± 0.059	2.218TyrSer: 2.218 ± 0.06	2.085TyrThr: 2.085 ± 0.059	2.217TyrVal: 2.217 ± 0.058	0.305TyrTrp: 0.305 ± 0.017	1.452TyrTyr: 1.452 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2248 proteins (719107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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