Synechococcus phage S-CAM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Anaposvirus; Synechococcus virus SCAM1

Average proteome isoelectric point is 5.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QRV9|M4QRV9_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM1 OX=754037 GN=C030809_014 PE=4 SV=1
MM1 pKa = 7.66PSINISWQRR10 pKa = 11.84SAGDD14 pKa = 3.29SNYY17 pKa = 9.94IYY19 pKa = 10.79GMPGGTIGPNSGSRR33 pKa = 11.84SVNVGFGQTYY43 pKa = 9.67NLSSSGSGPGNTALRR58 pKa = 11.84RR59 pKa = 11.84LNSQTLGLDD68 pKa = 3.47DD69 pKa = 4.2RR70 pKa = 11.84QGAGADD76 pKa = 3.23NDD78 pKa = 4.03YY79 pKa = 11.24NDD81 pKa = 3.92MIVYY85 pKa = 9.89VSGGGTFTGNSTFSGPPATYY105 pKa = 10.72GCMDD109 pKa = 4.43SNAVNYY115 pKa = 10.15NSSANVNSGCIYY127 pKa = 10.91ANPNPQLTVNGSTATQTIVEE147 pKa = 4.38GDD149 pKa = 4.27AITVSWSANDD159 pKa = 3.51SQYY162 pKa = 10.65MYY164 pKa = 9.36TGSISGQGAPGSLSSSQYY182 pKa = 10.38GGGSFVANPTSNTTYY197 pKa = 9.65TYY199 pKa = 10.11SVSYY203 pKa = 10.7APPTRR208 pKa = 11.84NDD210 pKa = 3.37SFSVPVNVKK219 pKa = 9.66EE220 pKa = 3.94IPEE223 pKa = 4.47IIASFPNGSTILRR236 pKa = 11.84GNSTNLVWSTSGDD249 pKa = 3.43ATTMSISPGLGLQNLSGTLSLSPTEE274 pKa = 4.13TTTYY278 pKa = 9.81TLYY281 pKa = 10.89ASSPGYY287 pKa = 10.23GGRR290 pKa = 11.84LQDD293 pKa = 4.18SVSLLLTVIQPPSASLTIPSTIDD316 pKa = 2.74WGDD319 pKa = 3.31SSFQAILEE327 pKa = 4.06FDD329 pKa = 3.66EE330 pKa = 4.44VTSYY334 pKa = 11.8DD335 pKa = 3.59LTVEE339 pKa = 4.14YY340 pKa = 10.54TDD342 pKa = 4.75LDD344 pKa = 3.9GVMITHH350 pKa = 7.48PAFTGADD357 pKa = 3.56PSQTTVNLLIGDD369 pKa = 4.17EE370 pKa = 4.41TSGTIPRR377 pKa = 11.84WNNRR381 pKa = 11.84GYY383 pKa = 10.49SQGKK387 pKa = 9.54VKK389 pKa = 10.15MKK391 pKa = 10.49AYY393 pKa = 10.16GLGGQFVEE401 pKa = 5.15KK402 pKa = 10.61EE403 pKa = 4.24SIFNINIDD411 pKa = 3.79QMPDD415 pKa = 3.31AIDD418 pKa = 3.67IPSSEE423 pKa = 4.35DD424 pKa = 3.01KK425 pKa = 11.01FLGEE429 pKa = 4.62EE430 pKa = 4.11PVITPDD436 pKa = 3.2VTVTSEE442 pKa = 3.99QIVIDD447 pKa = 4.26DD448 pKa = 3.24VDD450 pKa = 3.74IPVEE454 pKa = 4.46VKK456 pKa = 10.38ASSPIQVEE464 pKa = 3.83IDD466 pKa = 4.59DD467 pKa = 4.28GGVWYY472 pKa = 10.43NVRR475 pKa = 11.84QTT477 pKa = 3.32

Molecular weight: 50.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QRR5|M4QRR5_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM1 OX=754037 GN=C030809_067 PE=4 SV=1
MM1 pKa = 7.58INVSCISPRR10 pKa = 11.84PSRR13 pKa = 11.84SVWTLKK19 pKa = 10.63VNPFTGTCAVRR30 pKa = 11.84WFKK33 pKa = 10.57TPSAEE38 pKa = 4.16YY39 pKa = 9.74TFKK42 pKa = 10.48TRR44 pKa = 11.84KK45 pKa = 9.36RR46 pKa = 11.84DD47 pKa = 3.33ILALMMAGDD56 pKa = 4.06RR57 pKa = 11.84SLGQWVNYY65 pKa = 9.48HH66 pKa = 5.52MAA68 pKa = 4.45

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

231

0

231

62223

49

3693

269.4

29.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.878 ± 0.258

0.963 ± 0.125

6.711 ± 0.147

6.147 ± 0.206

4.37 ± 0.139

8.174 ± 0.287

1.589 ± 0.115

6.223 ± 0.17

5.495 ± 0.3

7.094 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.17

5.628 ± 0.172

4.179 ± 0.113

3.574 ± 0.106

4.105 ± 0.103

7.033 ± 0.22

7.394 ± 0.297

6.792 ± 0.153

1.176 ± 0.082

4.344 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski