Wenzhou narna-like virus 7
Average proteome isoelectric point is 8.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIT8|A0A1L3KIT8_9VIRU RNA-dependent RNA polymerase OS=Wenzhou narna-like virus 7 OX=1923582 PE=4 SV=1
MM1 pKa = 7.44 DD2 pKa = 4.22 QEE4 pKa = 4.9 FPCEE8 pKa = 3.84 RR9 pKa = 11.84 KK10 pKa = 9.17 EE11 pKa = 4.64 ALLDD15 pKa = 4.34 HH16 pKa = 7.06 INMKK20 pKa = 10.21 IMSKK24 pKa = 10.14 LRR26 pKa = 11.84 NEE28 pKa = 4.04 ARR30 pKa = 11.84 HH31 pKa = 5.0 KK32 pKa = 10.05 RR33 pKa = 11.84 KK34 pKa = 9.56 RR35 pKa = 11.84 AIGFAYY41 pKa = 10.38 SIQNLKK47 pKa = 10.55 YY48 pKa = 10.73 LITFEE53 pKa = 4.57 PKK55 pKa = 10.52 DD56 pKa = 4.07 LIKK59 pKa = 10.4 STLEE63 pKa = 3.44 KK64 pKa = 10.66 HH65 pKa = 6.19 RR66 pKa = 11.84 ADD68 pKa = 3.24 LSKK71 pKa = 10.63 PIGGLDD77 pKa = 3.61 PQIKK81 pKa = 9.25 EE82 pKa = 4.11 AYY84 pKa = 10.13 DD85 pKa = 4.14 FILQKK90 pKa = 10.93 FMDD93 pKa = 5.75 FIASNASEE101 pKa = 3.69 IDD103 pKa = 3.73 VFPEE107 pKa = 3.82 PTEE110 pKa = 4.22 SRR112 pKa = 11.84 CHH114 pKa = 6.0 SKK116 pKa = 9.07 TGKK119 pKa = 9.6 SFKK122 pKa = 9.87 EE123 pKa = 3.99 AFMKK127 pKa = 9.69 ATSEE131 pKa = 4.09 HH132 pKa = 6.39 TSLCSNYY139 pKa = 9.63 HH140 pKa = 5.38 IRR142 pKa = 11.84 VGDD145 pKa = 3.63 RR146 pKa = 11.84 TLCVPDD152 pKa = 3.39 TNYY155 pKa = 11.08 NKK157 pKa = 10.48 DD158 pKa = 3.12 FNFAHH163 pKa = 7.25 GDD165 pKa = 3.61 CLLNNTVKK173 pKa = 10.6 AVALVEE179 pKa = 4.01 PLKK182 pKa = 11.06 VRR184 pKa = 11.84 VITKK188 pKa = 8.8 EE189 pKa = 3.81 AAWTHH194 pKa = 4.87 MLKK197 pKa = 10.47 PIQKK201 pKa = 9.39 LMHH204 pKa = 6.45 SALTHH209 pKa = 6.39 HH210 pKa = 7.36 DD211 pKa = 3.52 FGEE214 pKa = 4.47 IFCLTRR220 pKa = 11.84 GKK222 pKa = 9.83 PYY224 pKa = 10.83 EE225 pKa = 4.15 NVSLGTLSDD234 pKa = 3.84 SEE236 pKa = 5.14 FYY238 pKa = 11.22 LSGDD242 pKa = 3.89 FSAATDD248 pKa = 3.89 NIHH251 pKa = 6.53 PHH253 pKa = 6.15 FLNEE257 pKa = 3.43 FRR259 pKa = 11.84 DD260 pKa = 3.68 RR261 pKa = 11.84 MRR263 pKa = 11.84 VILKK267 pKa = 7.87 DD268 pKa = 3.53 HH269 pKa = 6.22 PMIWHH274 pKa = 7.33 ALNSEE279 pKa = 4.02 ARR281 pKa = 11.84 NHH283 pKa = 6.25 IIEE286 pKa = 4.55 YY287 pKa = 9.52 PLGIPPVEE295 pKa = 4.13 QMRR298 pKa = 11.84 GQLMGSLLSFPILCLSNLAIVLASCRR324 pKa = 11.84 EE325 pKa = 4.22 HH326 pKa = 7.39 RR327 pKa = 11.84 DD328 pKa = 3.16 HH329 pKa = 7.22 TIDD332 pKa = 4.83 RR333 pKa = 11.84 VIQSASSRR341 pKa = 11.84 TGYY344 pKa = 10.55 KK345 pKa = 10.2 PIIEE349 pKa = 4.26 KK350 pKa = 10.8 VKK352 pKa = 9.5 ITISEE357 pKa = 4.12 NWSSTLEE364 pKa = 3.98 RR365 pKa = 11.84 LKK367 pKa = 10.91 SSCLINGDD375 pKa = 3.89 DD376 pKa = 4.51 LAIRR380 pKa = 11.84 TNDD383 pKa = 3.16 QFLSKK388 pKa = 10.14 WKK390 pKa = 10.31 VLSPNAGFTLSIGKK404 pKa = 8.82 NFIDD408 pKa = 4.24 KK409 pKa = 10.46 RR410 pKa = 11.84 FYY412 pKa = 10.55 TINSKK417 pKa = 10.77 LFIDD421 pKa = 4.46 DD422 pKa = 5.24 KK423 pKa = 11.21 IFSTHH428 pKa = 6.0 KK429 pKa = 10.06 FKK431 pKa = 11.23 CSMSNKK437 pKa = 9.61 GDD439 pKa = 3.8 LASLGAEE446 pKa = 3.79 YY447 pKa = 9.7 PRR449 pKa = 11.84 FFKK452 pKa = 10.85 TEE454 pKa = 3.77 GVQQEE459 pKa = 4.66 VVDD462 pKa = 3.43 KK463 pKa = 10.76 HH464 pKa = 5.65 NRR466 pKa = 11.84 TIRR469 pKa = 11.84 SMFIRR474 pKa = 11.84 DD475 pKa = 3.17 HH476 pKa = 6.76 HH477 pKa = 6.57 KK478 pKa = 10.86 RR479 pKa = 11.84 LMKK482 pKa = 10.39 DD483 pKa = 3.19 PRR485 pKa = 11.84 SLEE488 pKa = 4.19 VPRR491 pKa = 11.84 HH492 pKa = 4.96 LGGLGFGNLIPCNIMQKK509 pKa = 8.64 LTHH512 pKa = 7.14 LIYY515 pKa = 10.37 SYY517 pKa = 11.07 KK518 pKa = 8.81 ITKK521 pKa = 9.52 SPKK524 pKa = 9.69 NFTNLLSRR532 pKa = 11.84 FGLEE536 pKa = 3.07 RR537 pKa = 11.84 VYY539 pKa = 10.98 VPRR542 pKa = 11.84 GTCHH546 pKa = 7.15 RR547 pKa = 11.84 LKK549 pKa = 10.9 LCDD552 pKa = 3.4 YY553 pKa = 8.98 TSYY556 pKa = 10.78 IGLQFDD562 pKa = 3.85 EE563 pKa = 4.71 PTRR566 pKa = 11.84 DD567 pKa = 3.17 ISPSEE572 pKa = 3.79 LPVRR576 pKa = 11.84 IRR578 pKa = 11.84 QYY580 pKa = 10.91 IKK582 pKa = 10.63 LVKK585 pKa = 9.23 TKK587 pKa = 10.53 QLALTDD593 pKa = 4.07 RR594 pKa = 11.84 WFHH597 pKa = 5.37 WEE599 pKa = 3.52 WVIVRR604 pKa = 11.84 KK605 pKa = 9.66 FDD607 pKa = 4.3 LPIIRR612 pKa = 11.84 QFLVDD617 pKa = 4.46 LRR619 pKa = 11.84 KK620 pKa = 10.07 LGYY623 pKa = 10.6 DD624 pKa = 3.25 KK625 pKa = 11.07 AISKK629 pKa = 9.99 NMSYY633 pKa = 10.02 IGQVIQRR640 pKa = 11.84 LPTLANDD647 pKa = 3.29 YY648 pKa = 11.25 AKK650 pKa = 10.11 FVKK653 pKa = 10.06 RR654 pKa = 11.84 IYY656 pKa = 9.85 MKK658 pKa = 10.77 RR659 pKa = 11.84 LPKK662 pKa = 10.02 KK663 pKa = 9.99 VNGAYY668 pKa = 9.6 KK669 pKa = 10.16 VFRR672 pKa = 11.84 DD673 pKa = 3.62 SLHH676 pKa = 4.95 YY677 pKa = 9.55 TINTKK682 pKa = 9.53 VEE684 pKa = 3.82 PHH686 pKa = 6.2 TEE688 pKa = 3.77 LGKK691 pKa = 9.67 ATVVALNYY699 pKa = 10.05 AVRR702 pKa = 11.84 PTLPGNTCPYY712 pKa = 10.45 DD713 pKa = 3.08 SWSPFDD719 pKa = 3.85 GDD721 pKa = 3.1 VRR723 pKa = 11.84 LIVV726 pKa = 3.42
Molecular weight: 83.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.861
IPC2_protein 8.799
IPC_protein 8.653
Toseland 9.458
ProMoST 9.224
Dawson 9.736
Bjellqvist 9.472
Wikipedia 9.882
Rodwell 10.072
Grimsley 9.794
Solomon 9.75
Lehninger 9.706
Nozaki 9.633
DTASelect 9.414
Thurlkill 9.589
EMBOSS 9.897
Sillero 9.692
Patrickios 5.143
IPC_peptide 9.75
IPC2_peptide 8.097
IPC2.peptide.svr19 7.876
Protein with the highest isoelectric point:
>tr|A0A1L3KIT8|A0A1L3KIT8_9VIRU RNA-dependent RNA polymerase OS=Wenzhou narna-like virus 7 OX=1923582 PE=4 SV=1
MM1 pKa = 7.44 DD2 pKa = 4.22 QEE4 pKa = 4.9 FPCEE8 pKa = 3.84 RR9 pKa = 11.84 KK10 pKa = 9.17 EE11 pKa = 4.64 ALLDD15 pKa = 4.34 HH16 pKa = 7.06 INMKK20 pKa = 10.21 IMSKK24 pKa = 10.14 LRR26 pKa = 11.84 NEE28 pKa = 4.04 ARR30 pKa = 11.84 HH31 pKa = 5.0 KK32 pKa = 10.05 RR33 pKa = 11.84 KK34 pKa = 9.56 RR35 pKa = 11.84 AIGFAYY41 pKa = 10.38 SIQNLKK47 pKa = 10.55 YY48 pKa = 10.73 LITFEE53 pKa = 4.57 PKK55 pKa = 10.52 DD56 pKa = 4.07 LIKK59 pKa = 10.4 STLEE63 pKa = 3.44 KK64 pKa = 10.66 HH65 pKa = 6.19 RR66 pKa = 11.84 ADD68 pKa = 3.24 LSKK71 pKa = 10.63 PIGGLDD77 pKa = 3.61 PQIKK81 pKa = 9.25 EE82 pKa = 4.11 AYY84 pKa = 10.13 DD85 pKa = 4.14 FILQKK90 pKa = 10.93 FMDD93 pKa = 5.75 FIASNASEE101 pKa = 3.69 IDD103 pKa = 3.73 VFPEE107 pKa = 3.82 PTEE110 pKa = 4.22 SRR112 pKa = 11.84 CHH114 pKa = 6.0 SKK116 pKa = 9.07 TGKK119 pKa = 9.6 SFKK122 pKa = 9.87 EE123 pKa = 3.99 AFMKK127 pKa = 9.69 ATSEE131 pKa = 4.09 HH132 pKa = 6.39 TSLCSNYY139 pKa = 9.63 HH140 pKa = 5.38 IRR142 pKa = 11.84 VGDD145 pKa = 3.63 RR146 pKa = 11.84 TLCVPDD152 pKa = 3.39 TNYY155 pKa = 11.08 NKK157 pKa = 10.48 DD158 pKa = 3.12 FNFAHH163 pKa = 7.25 GDD165 pKa = 3.61 CLLNNTVKK173 pKa = 10.6 AVALVEE179 pKa = 4.01 PLKK182 pKa = 11.06 VRR184 pKa = 11.84 VITKK188 pKa = 8.8 EE189 pKa = 3.81 AAWTHH194 pKa = 4.87 MLKK197 pKa = 10.47 PIQKK201 pKa = 9.39 LMHH204 pKa = 6.45 SALTHH209 pKa = 6.39 HH210 pKa = 7.36 DD211 pKa = 3.52 FGEE214 pKa = 4.47 IFCLTRR220 pKa = 11.84 GKK222 pKa = 9.83 PYY224 pKa = 10.83 EE225 pKa = 4.15 NVSLGTLSDD234 pKa = 3.84 SEE236 pKa = 5.14 FYY238 pKa = 11.22 LSGDD242 pKa = 3.89 FSAATDD248 pKa = 3.89 NIHH251 pKa = 6.53 PHH253 pKa = 6.15 FLNEE257 pKa = 3.43 FRR259 pKa = 11.84 DD260 pKa = 3.68 RR261 pKa = 11.84 MRR263 pKa = 11.84 VILKK267 pKa = 7.87 DD268 pKa = 3.53 HH269 pKa = 6.22 PMIWHH274 pKa = 7.33 ALNSEE279 pKa = 4.02 ARR281 pKa = 11.84 NHH283 pKa = 6.25 IIEE286 pKa = 4.55 YY287 pKa = 9.52 PLGIPPVEE295 pKa = 4.13 QMRR298 pKa = 11.84 GQLMGSLLSFPILCLSNLAIVLASCRR324 pKa = 11.84 EE325 pKa = 4.22 HH326 pKa = 7.39 RR327 pKa = 11.84 DD328 pKa = 3.16 HH329 pKa = 7.22 TIDD332 pKa = 4.83 RR333 pKa = 11.84 VIQSASSRR341 pKa = 11.84 TGYY344 pKa = 10.55 KK345 pKa = 10.2 PIIEE349 pKa = 4.26 KK350 pKa = 10.8 VKK352 pKa = 9.5 ITISEE357 pKa = 4.12 NWSSTLEE364 pKa = 3.98 RR365 pKa = 11.84 LKK367 pKa = 10.91 SSCLINGDD375 pKa = 3.89 DD376 pKa = 4.51 LAIRR380 pKa = 11.84 TNDD383 pKa = 3.16 QFLSKK388 pKa = 10.14 WKK390 pKa = 10.31 VLSPNAGFTLSIGKK404 pKa = 8.82 NFIDD408 pKa = 4.24 KK409 pKa = 10.46 RR410 pKa = 11.84 FYY412 pKa = 10.55 TINSKK417 pKa = 10.77 LFIDD421 pKa = 4.46 DD422 pKa = 5.24 KK423 pKa = 11.21 IFSTHH428 pKa = 6.0 KK429 pKa = 10.06 FKK431 pKa = 11.23 CSMSNKK437 pKa = 9.61 GDD439 pKa = 3.8 LASLGAEE446 pKa = 3.79 YY447 pKa = 9.7 PRR449 pKa = 11.84 FFKK452 pKa = 10.85 TEE454 pKa = 3.77 GVQQEE459 pKa = 4.66 VVDD462 pKa = 3.43 KK463 pKa = 10.76 HH464 pKa = 5.65 NRR466 pKa = 11.84 TIRR469 pKa = 11.84 SMFIRR474 pKa = 11.84 DD475 pKa = 3.17 HH476 pKa = 6.76 HH477 pKa = 6.57 KK478 pKa = 10.86 RR479 pKa = 11.84 LMKK482 pKa = 10.39 DD483 pKa = 3.19 PRR485 pKa = 11.84 SLEE488 pKa = 4.19 VPRR491 pKa = 11.84 HH492 pKa = 4.96 LGGLGFGNLIPCNIMQKK509 pKa = 8.64 LTHH512 pKa = 7.14 LIYY515 pKa = 10.37 SYY517 pKa = 11.07 KK518 pKa = 8.81 ITKK521 pKa = 9.52 SPKK524 pKa = 9.69 NFTNLLSRR532 pKa = 11.84 FGLEE536 pKa = 3.07 RR537 pKa = 11.84 VYY539 pKa = 10.98 VPRR542 pKa = 11.84 GTCHH546 pKa = 7.15 RR547 pKa = 11.84 LKK549 pKa = 10.9 LCDD552 pKa = 3.4 YY553 pKa = 8.98 TSYY556 pKa = 10.78 IGLQFDD562 pKa = 3.85 EE563 pKa = 4.71 PTRR566 pKa = 11.84 DD567 pKa = 3.17 ISPSEE572 pKa = 3.79 LPVRR576 pKa = 11.84 IRR578 pKa = 11.84 QYY580 pKa = 10.91 IKK582 pKa = 10.63 LVKK585 pKa = 9.23 TKK587 pKa = 10.53 QLALTDD593 pKa = 4.07 RR594 pKa = 11.84 WFHH597 pKa = 5.37 WEE599 pKa = 3.52 WVIVRR604 pKa = 11.84 KK605 pKa = 9.66 FDD607 pKa = 4.3 LPIIRR612 pKa = 11.84 QFLVDD617 pKa = 4.46 LRR619 pKa = 11.84 KK620 pKa = 10.07 LGYY623 pKa = 10.6 DD624 pKa = 3.25 KK625 pKa = 11.07 AISKK629 pKa = 9.99 NMSYY633 pKa = 10.02 IGQVIQRR640 pKa = 11.84 LPTLANDD647 pKa = 3.29 YY648 pKa = 11.25 AKK650 pKa = 10.11 FVKK653 pKa = 10.06 RR654 pKa = 11.84 IYY656 pKa = 9.85 MKK658 pKa = 10.77 RR659 pKa = 11.84 LPKK662 pKa = 10.02 KK663 pKa = 9.99 VNGAYY668 pKa = 9.6 KK669 pKa = 10.16 VFRR672 pKa = 11.84 DD673 pKa = 3.62 SLHH676 pKa = 4.95 YY677 pKa = 9.55 TINTKK682 pKa = 9.53 VEE684 pKa = 3.82 PHH686 pKa = 6.2 TEE688 pKa = 3.77 LGKK691 pKa = 9.67 ATVVALNYY699 pKa = 10.05 AVRR702 pKa = 11.84 PTLPGNTCPYY712 pKa = 10.45 DD713 pKa = 3.08 SWSPFDD719 pKa = 3.85 GDD721 pKa = 3.1 VRR723 pKa = 11.84 LIVV726 pKa = 3.42
Molecular weight: 83.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.861
IPC2_protein 8.799
IPC_protein 8.653
Toseland 9.458
ProMoST 9.224
Dawson 9.736
Bjellqvist 9.472
Wikipedia 9.882
Rodwell 10.072
Grimsley 9.794
Solomon 9.75
Lehninger 9.706
Nozaki 9.633
DTASelect 9.414
Thurlkill 9.589
EMBOSS 9.897
Sillero 9.692
Patrickios 5.143
IPC_peptide 9.75
IPC2_peptide 8.097
IPC2.peptide.svr19 7.876
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
726
726
726
726.0
83.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.821 ± 0.0
1.928 ± 0.0
5.785 ± 0.0
4.821 ± 0.0
5.234 ± 0.0
4.545 ± 0.0
3.857 ± 0.0
7.576 ± 0.0
8.402 ± 0.0
10.055 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.0
4.683 ± 0.0
4.821 ± 0.0
2.479 ± 0.0
6.474 ± 0.0
7.163 ± 0.0
5.51 ± 0.0
4.959 ± 0.0
1.102 ± 0.0
3.444 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here